SIST EN ISO 6887-3:2017/A1:2020

Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 3: Specific rules for the preparation of fish and fishery products - Amendment 1: Sample preparation for raw marine gastropods (ISO 6887-3:2017/Amd 1:2020)

Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 3: Spezifische Regeln für die Vorbereitung von Fisch und Fischerzeugnissen - Änderung 1: Probenvorbereitung von rohen Meeresgastropoden (ISO 6887-3:2017/Amd 1:2020)

No scope available

Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 3: Règles spécifiques pour la préparation des produits de la pêche - Amendement 1: Préparation des échantillons pour gastéropodes marins crus (ISO 6887-3:2017/Amd 1:2020)

Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 3. del: Posebna pravila za pripravo rib in ribjih izdelkov - Dopolnilo A1: Priprava vzorcev surovih morskih polžev (ISO 6887-3:2017/Amd 1:2020)

General Information

• Status: Published
• Public Enquiry End Date: 19-Mar-2019
• Publication Date: 11-Nov-2020
• ICS: 07.100.30 - Food microbiology
• ICS: 67.120.30 - Fish and fishery products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 19-Oct-2020
• Due Date: 24-Dec-2020
• Completion Date: 12-Nov-2020
• Ref Project: EN ISO 6887-3:2017/A1:2020 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 3: Specific rules for the preparation of fish and fishery products - Amendment 1: Sample preparation for raw marine gastropods (ISO 6887-3:2017/Amd 1:2020)

Relations

• Amends: SIST EN ISO 6887-3:2017 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 3: Specific rules for the preparation of fish and fishery products (ISO 6887-3:2017)
• Effective Date: 01-Dec-2020

Overview

EN ISO 6887-3:2017/A1:2020 is an amendment to Part 3 of the ISO 6887 series - Microbiology of the food chain: Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. This CEN-adopted amendment (approved October 2020, published nationally in December 2020) adds specific sample preparation rules for raw marine gastropods (e.g., whelks, periwinkles) and refines handling and temperature guidance for fish and fishery product samples. It is intended to ensure reproducible, traceable preparation of samples for microbiological testing.

Key topics and technical requirements

• Scope update: Adds procedures specific to raw marine gastropods to ISO 6887-3:2017.
• Temperature handling (clause 7.2.5): For samples received within 4 hours of collection, internal air/sample temperature should be lower than the temperature recorded at sampling time.
• Procedure cross-reference (clause 7.3): Laboratories are to use the specific procedures given in clauses 7.2.2, 7.2.4 and 7.2.5 for relevant sample types.
• Gastropod sample preparation (new clause 9.1.10):
  • Wash at least 10 individuals under running potable water and place on a sterile tray.
  • Extract the animal body using forceps, a winkle picker or shellfish picker; shells can be cracked with a hammer.
  • Dice the flesh and remove shell debris with forceps to ease homogenization.
  • Prepare an initial suspension of approximately 1 in 3 in diluent; homogenize using a rotary or peristaltic homogenizer (reference to clause 6.1).
  • After homogenization, add diluent to obtain an accurate 1 in 10 suspension for downstream microbiological tests.

Applications and users

This amendment is directly applicable to:

• Microbiology laboratories performing routine or regulatory testing of fish and fishery products, especially raw marine gastropods.
• Seafood producers and processors establishing sampling and testing protocols for product safety and quality control.
• Regulatory bodies and food safety auditors evaluating compliance with standardized microbiological sample preparation methods.
• Research institutions studying seafood-associated microorganisms where standardized sample handling is essential for comparable results.

Benefits include improved reproducibility of results, clearer handling/temperature requirements, and harmonized procedures across CEN/ISO member countries.

Related standards

• Part of the ISO 6887 series (other parts cover general rules and different food categories). Consult the ISO catalogue or national standards bodies for the complete set and any additional amendments.

Keywords: ISO 6887-3, EN ISO 6887-3:2017/A1:2020, microbiology of the food chain, sample preparation, raw marine gastropods, initial suspension, decimal dilutions, homogenization, seafood microbiology.

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2020
Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne
suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 3. del: Posebna
pravila za pripravo rib in ribjih izdelkov - Dopolnilo A1: Priprava vzorcev surovih
morskih polžev (ISO 6887-3:2017/Amd 1:2020)
Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 3: Specific rules for the
preparation of fish and fishery products - Amendment 1: Sample preparation for raw
marine gastropods (ISO 6887-3:2017/Amd 1:2020)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 3: Spezifische Regeln für die Vorbereitung von Fisch und
Fischerzeugnissen - Änderung 1: Probenvorbereitung von rohen Meeresgastropoden
(ISO 6887-3:2017/Amd 1:2020)
Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension
mère et des dilutions décimales en vue de l'examen microbiologique - Partie 3: Règles
spécifiques pour la préparation des produits de la pêche - Amendement 1: Préparation
des échantillons pour gastéropodes marins crus (ISO 6887-3:2017/Amd 1:2020)
Ta slovenski standard je istoveten z: EN ISO 6887-3:2017/A1:2020
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.120.30 Ribe in ribji proizvodi Fish and fishery products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 6887-3:2017/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2020
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination - Part 3: Specific rules for the
preparation of fish and fishery products - Amendment 1:
Sample preparation for raw marine gastropods (ISO 6887-
3:2017/Amd 1:2020)
Microbiologie de la chaîne alimentaire - Préparation Mikrobiologie der Lebensmittelkette - Vorbereitung
des échantillons, de la suspension mère et des dilutions von Untersuchungsproben und Herstellung von
décimales en vue de l'examen microbiologique - Partie Erstverdünnungen und von Dezimalverdünnungen für
3: Règles spécifiques pour la préparation des produits mikrobiologische Untersuchungen - Teil 3: Spezifische
de la pêche - Amendement 1: Préparation des Regeln für die Vorbereitung von Fisch und
échantillons pour gastéropodes marins crus (ISO 6887- Fischerzeugnissen - Änderung 1: Probenvorbereitung
3:2017/Amd 1:2020) von rohen Meeresgastropoden (ISO 6887-3:2017/Amd
1:2020)
This amendment A1 modifies the European Standard EN ISO 6887-3:2017; it was approved by CEN on 4 October 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6887-3:2017/A1:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 6887-3:2017/Amd 1:2020 has been prepared by Technical Committee ISO/TC 34 "Food
products” of the International Organization for Standardization (ISO) and has been taken over as
the secretariat of which is held by AFNOR.
This Amendment to the European Standard EN ISO 6887-3:2017 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by April 2021, and
conflicting national standards shall be withdrawn at the latest by April 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Icel
...