Water quality - Multi-compound class methods - Part 2: Criteria for the quantitative determination of organic substances using a multi-compound class analytical method (ISO 21253-2:2019)

This  document specifies the critical issues to address when developing in a laboratory a method for the simultaneous quantitative analysis of numerous organic compounds in water.

Wasserbeschaffenheit - Gemeinsam erfassbare Stoffgruppen - Teil 2: Kriterien für die Beurteilung eines Multianalyt-Verfahrens organischer Stoffe (ISO 21253-2:2019)

Dieses Dokument legt die Kriterien für die Entwicklung einer massenspektrometrischen Inhouse-Methode zur quantitativen Analyse multipler Untergruppen organischer Stoffe im Rahmen der physikalisch-chemischen Untersuchung von Wasser fest.
Dieses Dokument ergänzt ISO/TS 13530, die Anleitung für die erstmalige Charakterisierung der Messleistungen bereitstellt, indem es detaillierte Angaben zur Auswahl der Testmatrix, der internen Standards und der Kriterien für die Wiederfindungen von Analyten und internen Standards macht.
Dieses Dokument ist nicht als Ersatz für die aktuell anzuwendenden Normen vorgesehen, die für die Analyse organischer Stoffe bestimmt sind, sondern vielmehr als Hilfsmittel, das weitere Charakterisierungselemente einbringt.

Qualité de l'eau - Méthodes d'analyse de composés multi-classes - Partie 2: Critères pour la détermination quantitative de composés organiques avec une méthode d'analyse de composés multi-classes (ISO 21253-2:2019)

Le présent document spécifie les critères pour le développement en interne d'une méthode fondée sur la spectrométrie de masse pour l'analyse quantitative de multiples sous-groupes de composés organiques dans le cadre d'une analyse physicochimique de l'eau.
Le présent document complète l'ISO/TS 13530 qui fournit des recommandations sur la caractérisation initiale des performances de mesure, en détaillant la sélection de la matrice d'essai et des étalons internes, ainsi que les critères pour la récupération de l'analyte et des étalons internes.
Le présent document n'a pas vocation à se substituer aux normes analytiques dédiées aux composés organiques actuellement en vigueur, mais est destiné à apporter des éléments de caractérisation supplémentaires.

Kakovost vode - Metode za več spojin - 2. del: Merila za kvantitativno določevanje organskih spojin z analizno metodo za več spojin (ISO 21253-2:2019)

Ta dokument določa kritična vprašanja, ki jih je treba obravnavati pri laboratorijski pripravi metode sočasne kvantitativne analize številnih organskih spojin v vodi.

General Information

Status
Published
Public Enquiry End Date
02-Jan-2019
Publication Date
02-Dec-2019
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
11-Nov-2019
Due Date
16-Jan-2020
Completion Date
03-Dec-2019

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SLOVENSKI STANDARD
SIST EN ISO 21253-2:2020
01-januar-2020

Kakovost vode - Metode za več spojin - 2. del: Merila za kvantitativno določevanje

organskih spojin z analizno metodo za več spojin (ISO 21253-2:2019)

Water quality - Multi-compound class methods - Part 2: Criteria for the quantitative

determination of organic substances using a multi-compound class analytical method

(ISO 21253-2:2019)

Wasserbeschaffenheit - Gemeinsam erfassbare Stoffgruppen - Teil 2: Kriterien für die

Beurteilung eines Multianalyt-Verfahrens organischer Stoffe (ISO 21253-2:2019)

Qualité de l'eau - Méthodes d'analyse de composés multi-classes - Partie 2: Critères

pour la détermination quantitative de composés organiques avec une méthode d'analyse

de composés multi-classes (ISO 21253-2:2019)
Ta slovenski standard je istoveten z: EN ISO 21253-2:2019
ICS:
13.060.50 Preiskava vode na kemične Examination of water for
snovi chemical substances
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 21253-2:2020 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 21253-2:2020
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SIST EN ISO 21253-2:2020
EN ISO 21253-2
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2019
EUROPÄISCHE NORM
ICS 13.060.50
English Version
Water quality - Multi-compound class methods - Part 2:
Criteria for the quantitative determination of organic
substances using a multi-compound class analytical
method (ISO 21253-2:2019)

Qualité de l'eau - Méthodes d'analyse de composés Wasserbeschaffenheit - Gemeinsam erfassbare

multi-classes - Partie 2: Critères pour la détermination Stoffgruppen - Teil 2: Kriterien für die Beurteilung

quantitative de composés organiques avec une eines Multianalyt-Verfahrens organischer Stoffe (ISO

méthode d'analyse de composés multi-classes (ISO 21253-2:2019)
21253-2:2019)
This European Standard was approved by CEN on 9 August 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21253-2:2019 E

worldwide for CEN national Members.
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SIST EN ISO 21253-2:2020
EN ISO 21253-2:2019 (E)
Contents Page

European foreword ....................................................................................................................................................... 3

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SIST EN ISO 21253-2:2020
EN ISO 21253-2:2019 (E)
European foreword

This document (EN ISO 21253-2:2019) has been prepared by Technical Committee ISO/TC 147 "Water

quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of

which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by April 2020, and conflicting national standards shall be

withdrawn at the latest by April 2020.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,

Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,

Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of

North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the

United Kingdom.
Endorsement notice

The text of ISO 21253-2:2019 has been approved by CEN as EN ISO 21253-2:2019 without any

modification.
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SIST EN ISO 21253-2:2020
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SIST EN ISO 21253-2:2020
INTERNATIONAL ISO
STANDARD 21253-2
First edition
2019-08
Water quality — Multi-compound
class methods —
Part 2:
Criteria for the quantitative
determination of organic substances
using a multi-compound class
analytical method
Qualité de l'eau — Méthodes d'analyse de composés multi-classes —
Partie 2: Critères pour la détermination quantitative de composés
organiques avec une méthode d'analyse de composés multi-classes
Reference number
ISO 21253-2:2019(E)
ISO 2019
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SIST EN ISO 21253-2:2020
ISO 21253-2:2019(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2019

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may

be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting

on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address

below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
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SIST EN ISO 21253-2:2020
ISO 21253-2:2019(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 3

5 Selection of the matrix ................................................................................................................................................................................... 3

6 Sample preservation prior to analysis........................................................................................................................................... 4

7 Internal standards and injection standards ............................................................................................................................ 4

7.1 General ........................................................................................................................................................................................................... 4

7.2 Selection of standards ....................................................................................................................................................................... 4

8 Calibration .................................................................................................................................................................................................................. 5

9 Recovery determination ............................................................................................................................................................................... 6

9.1 General ........................................................................................................................................................................................................... 6

9.2 Quantification by external calibration ................................................................................................................................ 6

9.3 Quantification by internal calibration ................................................................................................................................. 7

10 Limit of quantification (LOQ) .................................................................................................................................................................. 7

11 Results ............................................................................................................................................................................................................................. 7

11.1 Identification of the compounds .............................................................................................................................................. 7

11.2 Quantification .......................................................................................................................................................................................... 7

11.3 Measurement uncertainty ............................................................................................................................................................. 7

12 Quality controls ..................................................................................................................................................................................................... 7

12.1 General ........................................................................................................................................................................................................... 7

12.2 Quality control checks on the blank ..................................................................................................................................... 8

12.3 Quality control checks on the internal standards ..................................................................................................... 8

12.4 Quality control checks on the limit of quantification............................................................................................. 8

Annex A (informative) Matrix effect ...................................................................................................................................................................... 9

Bibliography .............................................................................................................................................................................................................................10

© ISO 2019 – All rights reserved iii
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SIST EN ISO 21253-2:2020
ISO 21253-2:2019(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO's adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following

URL: www .iso .org/iso/foreword .html.

This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2,

Physical, chemical and biochemical methods.
A list of all parts in the ISO 21253 series can be found on the ISO website.

Any feedback or questions on this document should be directed to the user’s national standards body. A

complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved
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SIST EN ISO 21253-2:2020
INTERNATIONAL STANDARD ISO 21253-2:2019(E)
Water quality — Multi-compound class methods —
Part 2:
Criteria for the quantitative determination of organic
substances using a multi-compound class analytical method
1 Scope

This document specifies the criteria for developing an in-house mass spectrometry-based method for

quantitative analysis of multiple subgroups of organic substances in the scope of physical-chemical

analysis of water.

This document supplements ISO/TS 13530 which provides guidance on the initial characterization of

the measurement performances, by providing details to select the test matrix and internal standards

and criteria for analyte and internal standard recoveries.

This document is not intended as a substitute for the currently applicable analytical standards

dedicated to organic compounds but as a resource bringing additional characterization elements.

2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

ISO 8466-1, Water quality — Calibration and evaluation of analytical methods and estimation of

performance characteristics — Part 1: Statistical evaluation of the linear calibration function

ISO 8466-2, Water quality — Calibration and evaluation of analytical methods and estimation of

performance characteristics — Part 2: Calibration strategy for non-linear second-order calibration

functions

ISO 11352, Water quality — Estimation of measurement uncertainty based on validation and quality

control data

ISO 21253-1, Water quality — Multi- compound class methods — Part 1: Criteria for the identification of

target compounds by gas and liquid chromatography and mass spectrometry
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— ISO Online browsing platform: available at https: //www .iso .org ./obp
— IEC Electropedia: available at http: //www .electropedia .org/
3.1
analyte
substance to be determined
[SOURCE: ISO/TS 28581:2012, 3.1]
© ISO 2019 – All rights reserved 1
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SIST EN ISO 21253-2:2020
ISO 21253-2:2019(E)
3.2
blank

aliquot of reagent water (reagent blank) or of a matrix in which the analyte (3.1) is absent (matrix blank)

that is treated exactly as a sample through the complete analytical procedure including extraction,

clean-up, identification and quantification including all the relevant reagents and materials

Note 1 to entry: It is crucial that the laboratory specifies which blank is considered.

3.3
calibration curve

expression of the relation between indication and corresponding measured quantity value

[SOURCE: ISO/IEC Guide 99:2007, 4.31]
3.4
limit of quantification
LOQ

lowest value of a determinand that can be determined with an acceptable level of accuracy, which could

be estimated by different means and shall be verified in the intended matrix
[SOURCE: ISO/TS 21231:2019, 3.2.5, modified — Note to entry has been excluded.]
3.5
analytical method

unambiguously written procedure describing all details required to carry out the analysis of the

determinand or parameter, namely: scope and field of application, principle and/or reactions,

definitions, reagents, apparatus, analytical procedures, calculations and presentation of results,

performance data and test report
[SOURCE: ISO/TS 16489:2006, 3.3]
3.6
recovery
relative recovery

extent to which a known, added quantity of determinand in a sample can be measured by an

analytical system

Note 1 to entry: Recovery is calculated from the difference between results obtained from a spiked and an

unspiked aliquot of sample and is usually expressed as a percentage.
[SOURCE: ISO 5667-14:2014, 3.8]
3.7
relative retention time

ratio between the retention time of the target compound and the retention time of the calibration

standard

[SOURCE: ISO 15680:2003, 3.5, modified — "retention-time standard" has been replaced by "calibration

standard"]
3.8
injection standard

standard mixture added to a sample before injection into the GC-MS apparatus, to monitor variability

of instrument response and to calculate internal standard recovery

Note 1 to entry: The same definition is applied for GC-MS/MS, LC-MS and LC-MS/MS.

[SOURCE: ISO 28540:2011, 3.4, modified — Note to entry has been replaced.]
2 © ISO 2019 – All rights reserved
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SIST EN ISO 21253-2:2020
ISO 21253-2:2019(E)
3.9
subgroup of compounds

analytes (3.1) pre-classified into groups that share similar patterns of analytical behaviour in response

to extraction mode and analysis
3.10
internal standard

compound added in a known amount to the sample from the beginning of the protocol and enabling

analytical coverage throughout the procedure, and that is used to correct for losses during sample

preparation and analysis by accounting for all-system matrix effects (recoveries, ionization effect,

variability of the detector response of the instrument for example)

Note 1 to entry: Determining the ratio of characteristic molecule signal intensity to the internal standard makes

it possible to calculate the quantitative ratio between molecule and internal standard and thereby to deduce

molecule concentration in
...

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