SIST EN ISO 13720:2011

Meat and meat products - Enumeration of presumptive Pseudomonas spp. (ISO 13720:2010)

ISO 13720:2010 specifies a method for the enumeration of presumptive Pseudomonas spp. present in meat and meat products, including poultry.

Fleisch und Fleischerzeugnisse - Zählung von präsumtiven Pseudomonas spp. (ISO 13720:2010)

Diese Internationale Norm beschreibt ein Verfahren für die Zählung von präsumtiven Pseudomonas spp. in
Fleisch und Fleischerzeugnissen einschließlich Geflügel.

Viande et produits à base de viande - Dénombrement des Pseudomonas spp. présomptifs (ISO 13720:2010)

L'ISO 13720:2010 spécifie une méthode pour le dénombrement des Pseudomonas spp. présomptifs présents dans la viande et les produits à base de viande, y compris les volailles.

Meso in mesni proizvodi - Štetje domnevno prisotnih Pseudomonas spp. (ISO 13720:2010)

Ta mednarodni standard določa metodo za štetje domnevno prisotnih Pseudomas spp., prisotnih v mesu in mesnih proizvodih, vključno s perutnino.

General Information

• Status: Published
• Publication Date: 03-Feb-2011
• ICS: 67.120.10 - Meat and meat products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 05-Jan-2011
• Due Date: 12-Mar-2011
• Completion Date: 04-Feb-2011
• Ref Project: EN ISO 13720:2010 - Meat and meat products - Enumeration of presumptive Pseudomonas spp. (ISO 13720:2010)

Overview - EN ISO 13720:2010 (Meat and meat products, Pseudomonas spp.)

EN ISO 13720:2010 specifies a validated microbiological method for the enumeration of presumptive Pseudomonas spp. in meat and meat products (including poultry). The standard defines the sampling preparation references, the selective culture medium (CFC agar), incubation conditions and a simple biochemical confirmation (oxidase test) to report presumptive Pseudomonas counts per gram or millilitre.

Key topics and technical requirements

• Scope: Enumeration of presumptive Pseudomonas spp. in raw and processed meat products and poultry.
• Sample preparation: Prepare initial suspensions and decimal dilutions in accordance with ISO 6887‑1 and ISO 6887‑2.
• Selective medium: Use Cephalothin‑Fusidate‑Cetrimide (CFC) agar - basic medium supplemented with cephalothin, sodium fusidate and cetrimide as inhibitors. Media preparation and quality checks follow ISO/TS 11133 guidelines.
• Inoculation & incubation: Spread 0.1 ml of appropriate dilutions onto CFC plates. Incubate at 25 °C ± 1 °C for 44 h ± 4 h. Retain plates with <150 colonies for counting.
• Confirmation: Select representative colonies (minimum five per retained plate) and perform an oxidase test (N,N,N',N'‑tetramethyl‑p‑phenylenediamine reagent). A violet/purple colour within 5–10 s indicates a positive oxidase reaction - colonies showing this are considered presumptive Pseudomonas spp.
• Performance & controls: The standard references productivity/selectivity performance testing (ISO/TS 11133‑2) and suggests use of positive/negative control strains during confirmation.
• Reporting: Results must identify the sample, reference EN ISO 13720:2010 method, state results, and record any deviations or operational details that could affect outcomes.

Applications and who should use it

• Food microbiology laboratories performing routine or regulatory testing for spoilage and hygiene indicators in meat and poultry.
• Meat processors and quality assurance teams monitoring microbial quality and shelf‑life risks associated with Pseudomonas spp.
• Regulatory bodies, inspection services and auditors requiring harmonized, comparable enumeration data across laboratories and countries.
• Research groups studying meat spoilage organisms or assessing antimicrobial/processing interventions.

Related standards (normative references)

• ISO 6887‑1 / ISO 6887‑2 - sample preparation and dilutions for microbiological examination
• ISO 7218 - general microbiology laboratory requirements
• ISO/TS 11133‑1 and ISO/TS 11133‑2 - preparation, QA and performance testing of culture media

EN ISO 13720:2010 provides a consistent, internationally‑recognized procedure for Pseudomonas enumeration in meat, supporting food safety, shelf‑life assessment and regulatory compliance through standardized culture, incubation and confirmation steps.

Standards Content (Sample)

SLOVENSKI STANDARD
01-marec-2011
Meso in mesni proizvodi - Štetje domnevno prisotnih Pseudomonas spp. (ISO
13720:2010)
Meat and meat products - Enumeration of presumptive Pseudomonas spp. (ISO
13720:2010)
Fleisch und Fleischerzeugnisse - Zählung von präsumtiven Pseudomonas spp. (ISO
13720:2010)
Viande et produits à base de viande - Dénombrement des Pseudomonas spp.
présomptifs (ISO 13720:2010)
Ta slovenski standard je istoveten z: EN ISO 13720:2010
ICS:
67.120.10 Meso in mesni proizvodi Meat and meat products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 13720
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2010
ICS 07.100.30; 67.120.10
English Version
Meat and meat products - Enumeration of presumptive
Pseudomonas spp. (ISO 13720:2010)
Viande et produits à base de viande - Dénombrement des Fleisch und Fleischerzeugnisse - Zählung von präsumtiven
Pseudomonas spp. présomptifs (ISO 13720:2010) Pseudomonas spp. (ISO 13720:2010)
This European Standard was approved by CEN on 20 February 2010.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13720:2010: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 13720:2010 has been prepared by Technical Committee ISO/TC 34 "Food products" of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 13720:2010 by
Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by March 2011, and conflicting national standards shall be withdrawn at
the latest by March 2011.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 13720:2010 has been approved by CEN as a EN ISO 13720:2010 without any modification.

INTERNATIONAL ISO
STANDARD 13720
Second edition
2010-08-15
Meat and meat products — Enumeration
of presumptive Pseudomonas spp.
Viande et produits à base de viande — Dénombrement des
Pseudomonas spp. présomptifs
Reference number
ISO 13720:2010(E)
©
ISO 2010
ISO 13720:2010(E)
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ISO 13720:2010(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 13720 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
This second edition cancels and replaces the first edition (ISO 13720:1995), which has been technically
revised.
INTERNATIONAL STANDARD ISO 13720:2010(E)

Meat and meat products — Enumeration of presumptive
Pseudomonas spp.
1 Scope
This International Standard specifies a method for the enumeration of presumptive Pseudomonas spp.
present in meat and meat products, including poultry.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial
suspension and decimal dilutions
ISO 6887-2, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and
meat products
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for
microbiological examinations
ISO/TS 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production
of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the
laboratory
ISO/TS 11133-2, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production
of culture media — Part 2: Practical guidelines on performance testing of culture media
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
presumptive Pseudomonas spp.
bacteria which at 25 °C form colonies in cephalothin-sodium fusidate-cetrimide (CFC) agar and which show a
positive oxidase reaction when tested according to the method described in this Int
...