ASTM E2362-09

Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection

SIGNIFICANCE AND USE
This test method may be used to determine if a pre-saturated or impregnated towelette demonstrates antimicrobial effectiveness as a disinfectant on hard surfaces.
SCOPE
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a hard surface disinfectant.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP’s) are required and to follow them when appropriate.  
1.3 This practice should be performed only by those trained in microbiological techniques.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Nov-2009
• ICS: 71.100.35 - Chemicals for industrial and domestic disinfection purposes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Relations

• Replaces: ASTM E2362-04 - Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection
• Effective Date: 01-Dec-2009
• Referred By: ASTM E2967-15 - Standard Test Method for Assessing the Ability of Pre-wetted Towelettes to Remove and Transfer Bacterial Contamination on Hard, Non-Porous Environmental Surfaces Using the Wiperator
• Effective Date: 01-Dec-2009

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2362 − 09
StandardPractice for
Evaluation of Pre-saturated or Impregnated Towelettes for
1
Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2 CFU, n—colony forming units
3.3 disinfectant, n—a physical or chemical agent or process
1.1 This practice is designed to evaluate the antimicrobial
thatdestroyspathogenicorpotentiallypathogenicmicroorgan-
activity of pre-saturated or impregnated towelettes when used
isms in/on surfaces or objects.
as a hard surface disinfectant.
3.4 impregnated, adj—saturated with test substance.
1.2 It is the responsibility of the investigator to determine
whether Good Laboratory Practices (GLP’s) are required and
3.5 neutralizer, n—a component used to render an active
to follow them when appropriate.
agent incapable of destroying organisms by chemical or
physical means.
1.3 This practice should be performed only by those trained
3.6 pre-saturated, adj—to be filled or impregnated with test
in microbiological techniques.
substance prior to the time of its intended use.
1.4 The values stated in SI units are to be regarded as
3.7 towelette, n—A paper, cloth or non-woven blend mate-
standard. No other units of measurement are included in this
rial used as a transporter for a cleaning and/or disinfection
standard.
agent.
1.5 This standard does not purport to address all of the
4
safety concerns, if any, associated with its use. It is the
4. Summary of Practice
responsibility of the user of this standard to establish appro-
4.1 A towelette impregnated or pre-saturated with a test
priate safety and health practices and to determine the
substance is used to treat a carrier which has been inoculated
applicability of regulatory limitations prior to use.
withatestorganismafteranaliquotofatestorganismhasbeen
inoculated, evenly distributed, and dried onto the carrier. The
2. Referenced Documents
carrier is wiped using the pre-saturated or impregnated tow-
2
2.1 ASTM Standards:
elette simulating the application of the test substance and then
D1193Specification for Reagent Water
held for a pre-determined contact time. After the specified
E1054Test Methods for Evaluation of Inactivators of Anti-
contact time, the test substance remaining on the carrier is
microbial Agents
neutralized and the carrier is subcultured to recover surviving
2.2 Federal Standard
testorganism.Theusedtowelette,afterthecontacttime,isalso
3
40 CFR, Part 160Good Laboratory Practice Standards
cultured for surviving test organism.
3. Terminology
5. Significance and Use
3.1 carrier, n—a transportable surface onto which a test
5.1 This test method may be used to determine if a pre-
organism will be inoculated and dried. The carrier will be
saturated or impregnated towelette demonstrates antimicrobial
treated with the test substance and subcultured for survivors.
effectiveness as a disinfectant on hard surfaces.
6. Apparatus
1
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides,
6.1 Incubator—any calibrated incubator that maintains a
Antimicrobials, and Alternative Control Agentsand is the direct responsibility of
temperature specific for propagation of organisms. (for
Subcommitee E35.15 on Antimicrobial Agents.
Current edition approved Dec. 1, 2009 Published December 2009. Originally
example, bacteria and mycobacteria at 35 62°C and fungi at
approved in 2004. Last previous edition approved in 2004 as E2362–04. DOI:
25 62°C).
10.1520/E2362-09.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
4
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM United States Environmental Protection Agency, Efficacy Data Requirements,
Standards volume information, refer to the standard’s Document Summary page on “Pre-Saturated or Impregnated Towelettes for Hard Surface Disinfection” Standard
the ASTM website. Operating Procedure for Testing of Towelette Disinfectants against Staphylococcus
3
Available from the Superintendent of Documents, U.S. Government Printing aureus and Pseudomonas aeruginosa, EPA/OPP Microbiology Laboratory, Ft.
Office, Washington D.C. 20402 Meade, MD. SOP# MB09-02, Revised 12/31/06.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E2362 − 09
6.2 Sterilizer—any suitable steam sterilizer that produces 7.1.5 Fluid Thioglycollate Broth.
the conditions of sterilization is acceptable.
7.2 Culture Media—Mycobacteria
6.3 Test Towele
...

This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2362–04 Designation: E2362 – 09
Standard Practice for
Evaluation of Pre-saturated or Impregnated Towelettes for
1
Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a
hard surface disinfectant.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP’s) are required and to
follow them when appropriate.
1.3 This practice should be performed only by those trained in microbiological techniques.
1.4
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2.1 ASTM Standards:
2
D1193ASTM Standards:
D1193 Specification for Reagent Water
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
2.2 Federal Standard
3
40 CFR, Part 160,Good160 Good Laboratory Practice Standards
3. Terminology
3.1 carrier, n—a transportable surface onto which a test organism will be inoculated and dried. The carrier will be treated with
the test substance and subcultured for survivors.
3.2 CFU, n—colony forming units
3.3 disinfectant, n—a physical or chemical agent or process that destroys pathogenic or potentially pathogenic microorganisms
in/on surfaces or objects.
3.4 impregnated, adj—saturated with test substance.
3.5 neutralizer, n—a component used to render an active agent incapable of destroying organisms by chemical or physical
means.
3.6 pre-saturated, adj—to be filled or impregnated with test substance prior to the time of its intended use.
3.7 towelette, n—Apaper,clothornon–wovennon-wovenblendmaterialusedasatransporterforacleaningand/ordisinfection
agent.
4
4. Summary of Practice
4.1 A towelette impregnated or pre-saturated with a test substance is used to treat a carrier which has been inoculated with a
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides and Alternative Control Agents and is the direct responsibility of Subcommitee E35.15
on Antimicrobial and Antiviral Agents. Current edition approved Oct. 1, 2004. Published October 2004. DOI: 10.1520/E2362-04.on Antimicrobial Agents.
Current edition approved Dec. 1, 2009 Published December 2009. Originally approved in 2004. Last previous edition approved in 2004 as E2362 – 04. DOI:
10.1520/E2362-09.
2
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from the Superintendent of Documents, U.S. Government Printing Office, Washington D.C. 20402
4
United States Environmental Protection Agency, Efficacy Data Requirements, “Pre-Saturated or Impregnated Towelettes for Hard Surface Disinfection” Standard
Operating Procedure for Testing of Towelette Disinfectants against Salmonella choleraesuis , Staphylococcus aureus, Pseudomonas aeruginosa, and Mycobacterium bovis
(BCG),EPA/OPPMicrobiologyLaboratory,Ft.Meade,MD.SOP#MB09-01,Revised11/08/00.StaphylococcusaureusandPseudomonasaeruginosa,EPA/OPPMicrobiology
Laboratory, Ft. Meade, MD. SOP# MB09-02, Revised 12/31/06.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

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E2362 – 09
test organism after an aliquot of a test organism has been inoculated, evenly distributed, and dried onto the carrier. The carrier is
wiped using the pre-saturated or impregnated towelette simulating the application of the test substance and then held for a
pre-determined contact time. After the specified contact time, the test substance remaining on the carrier is neutralized and the
carrier is subcultured to recover surviving test organism. The used towelette, after the cont
...