ASTM E2362-15

Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection

SIGNIFICANCE AND USE
5.1 This practice may be used to determine if a pre-saturated or impregnated towelette demonstrates antimicrobial effectiveness as a disinfectant on hard surfaces. This practice provides survivor results in the form of a qualitative endpoint (growth positive versus growth negative). The results generated by following this practice do not provide for specific quantitative reductions.
SCOPE
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a hard surface disinfectant.  
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP’s) are required and to follow them when appropriate.  
1.3 This practice should be performed only by those trained in microbiological techniques.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 Appropriate modifications to the practice may be required when testing organisms not specified herein.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Sep-2015
• ICS: 71.100.35 - Chemicals for industrial and domestic disinfection purposes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2362 − 15
Standard Practice for
Evaluation of Pre-saturated or Impregnated Towelettes for
1
Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Terminology
3.1 carrier, n—a transportable surface onto which a test
1.1 This practice is designed to evaluate the antimicrobial
organism will be inoculated and dried. The carrier will be
activity of pre-saturated or impregnated towelettes when used
treated with the test substance and subcultured for survivors.
as a hard surface disinfectant.
3.2 CFU, n—colony forming units
1.2 It is the responsibility of the investigator to determine
3.3 disinfectant, n—a physical or chemical agent or process
whether Good Laboratory Practices (GLP’s) are required and
thatdestroyspathogenicorpotentiallypathogenicmicroorgan-
to follow them when appropriate.
isms in/on surfaces or objects.
1.3 This practice should be performed only by those trained
3.4 impregnated, adj—saturated with test substance.
in microbiological techniques.
3.5 neutralizer, n—a component used to render an active
1.4 The values stated in SI units are to be regarded as
agent incapable of destroying organisms by chemical or
standard. No other units of measurement are included in this
physical means.
standard.
3.6 pre-saturated, adj—to be filled or impregnated with test
1.5 Appropriate modifications to the practice may be re- substance prior to the time of its intended use.
quired when testing organisms not specified herein.
3.7 towelette, n—A paper, cloth or non-woven blend mate-
rial used as a transporter for a cleaning and/or disinfection
1.6 This standard does not purport to address all of the
agent.
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro-
4
4. Summary of Practice
priate safety and health practices and to determine the
4.1 A towelette impregnated or pre-saturated with a test
applicability of regulatory limitations prior to use.
substance is used to treat a carrier which has been inoculated
withatestorganismafteranaliquotofatestorganismhasbeen
2. Referenced Documents
inoculated, evenly distributed to an inoculation area of ap-
2
2.1 ASTM Standards:
proximately one square inch (approximately 625 mm), and
D1193Specification for Reagent Water
dried onto the carrier. The carrier is wiped using the pre-
E1054Test Methods for Evaluation of Inactivators of Anti-
saturated or impregnated towelette simulating the application
microbial Agents
ofthetestsubstanceandthenheldforapre-determinedcontact
2.2 Federal Standard time. After the specified contact time, the test substance
3
remaining on the carrier is neutralized and the carrier is
40 CFR, Part 160Good Laboratory Practice Standards
subcultured to recover surviving test organism.
5. Significance and Use
1
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides,
Antimicrobials, and Alternative Control Agents and is the direct responsibility of 5.1 Thispracticemaybeusedtodetermineifapre-saturated
Subcommittee E35.15 on Antimicrobial Agents.
or impregnated towelette demonstrates antimicrobial effective-
Current edition approved Oct. 1, 2015 Published October 2015. Originally
ness as a disinfectant on hard surfaces. This practice provides
approved in 2004. Last previous edition approved in 2009 as E2362–09. DOI:
survivor results in the form of a qualitative endpoint (growth
10.1520/E2362-15.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
4
Standards volume information, refer to the standard’s Document Summary page on UnitedStatesEnvironmentalProtectionAgency,StandardOperatingProcedure
the ASTM website. for Disinfectant Towelette Test Against Staphylococcus aureus, Pseudomonas
3
Available from the Superintendent of Documents, U.S. Government Printing aeruginosa, and Salmonella enterica, EPA/OPP Microbiology Laboratory, Ft.
Office, Washington D.C. 20402 Meade, MD. SOP# MB09-05, Revised 1/30/13.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E2362 − 15
positive versus growth negative). The results generated by 6.27 Hot air oven—ability to maintain ≥180°C.
following this practice do not provide for specific quantitative
6.28 Refrigerator—calibrated to maintain 5 6 3°C.
reductions.
6.29 Ultra-Cold Freezer, Calibrated to maintain ≤ -70°C
6. Apparatu
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E2362 − 09 E2362 − 15
Standard Practice for
Evaluation of Pre-saturated or Impregnated Towelettes for
1
Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a
hard surface disinfectant.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP’s) are required and to
follow them when appropriate.
1.3 This practice should be performed only by those trained in microbiological techniques.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 Appropriate modifications to the practice may be required when testing organisms not specified herein.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
2.2 Federal Standard
3
40 CFR, Part 160 Good Laboratory Practice Standards
3. Terminology
3.1 carrier, n—a transportable surface onto which a test organism will be inoculated and dried. The carrier will be treated with
the test substance and subcultured for survivors.
3.2 CFU, n—colony forming units
3.3 disinfectant, n—a physical or chemical agent or process that destroys pathogenic or potentially pathogenic microorganisms
in/on surfaces or objects.
3.4 impregnated, adj—saturated with test substance.
3.5 neutralizer, n—a component used to render an active agent incapable of destroying organisms by chemical or physical
means.
3.6 pre-saturated, adj—to be filled or impregnated with test substance prior to the time of its intended use.
3.7 towelette, n—A paper, cloth or non-woven blend material used as a transporter for a cleaning and/or disinfection agent.
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
SubcommiteeSubcommittee E35.15 on Antimicrobial Agents.
Current edition approved Dec. 1, 2009Oct. 1, 2015 Published December 2009October 2015. Originally approved in 2004. Last previous edition approved in 20042009
as E2362 – 04.E2362 – 09. DOI: 10.1520/E2362-09.10.1520/E2362-15.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from the Superintendent of Documents, U.S. Government Printing Office, Washington D.C. 20402
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E2362 − 15
4
4. Summary of Practice
4.1 A towelette impregnated or pre-saturated with a test substance is used to treat a carrier which has been inoculated with a
test organism after an aliquot of a test organism has been inoculated, evenly distributed, distributed to an inoculation area of
approximately one square inch (approximately 625 mm), and dried onto the carrier. The carrier is wiped using the pre-saturated
or impregnated towelette simulating the application of the test substance and then held for a pre-determined contact time. After
the specified contact time, the test substance remaining on the carrier is neutralized and the carrier is subcultured to recover
surviving test organism. The used towelette, after the contact time, is also cultured for surviving test organism.
5. Significance and Use
5.1 This test method practice may be used to determine if a pre-saturated or impregnated towelette demonstrates antimicrobial
effectiveness as a disinfectant on hard surfaces. This practice provides survivor results in the form of a qualitative endpoint (growth
positive versus growth negative). The results generated
...