ASTM E1153-03

Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces

SCOPE
1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned inanimate, nonporous, non-food contact surfaces.
1.2 This test method may also be used to evaluate the antimicrobial efficacy of one-step cleaner/sanitizer formulations recommended for use on lightly soiled, inanimate, nonporous, non-food contact surfaces.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) is required and to follow them where appropriate (see section 40 CFR, 160) or as revised.
1.4 This standard may involve hazardous materials, chemicals and microorganisms and should be performed only by persons who have had formal microbiological training. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 09-Apr-2003
• ICS: 11.080.20 - Disinfectants and antiseptics
• ICS: 71.100.35 - Chemicals for industrial and domestic disinfection purposes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Relations

• Replaced By: ASTM E1153-03(2010)e1 - Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces
• Effective Date: 01-Apr-2010
• Referred By: ASTM E3135-18 - Standard Practice for Determining Antimicrobial Efficacy of Ultraviolet Germicidal Irradiation Against Microorganisms on Carriers with Simulated Soil
• Effective Date: 10-Apr-2003
• Referred By: ASTM E1053-20 - Standard Practice to Assess Virucidal Activity of Chemicals Intended for Disinfection of Inanimate, Nonporous Environmental Surfaces
• Effective Date: 10-Apr-2003
• Referred By: ASTM E3286-21 - Standard Practice for Preparation Of Cell Monolayers on Glass Surfaces for Evaluation of Microbicidal Properties of Non-Chemical Based Antimicrobial Treatment Technologies
• Effective Date: 10-Apr-2003

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information.
Designation: E1153 – 03
Standard Test Method for
Efficacy of Sanitizers Recommended for Inanimate Non-
1
Food Contact Surfaces
This standard is issued under the fixed designation E1153; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Significance and Use
1.1 This test method is used to evaluate the antimicrobial 3.1 Thistestmethodshallbeusedtodetermineifachemical
efficacy of sanitizers on precleaned inanimate, nonporous, has application as a non-food contact sanitizer or as a one-step
non-food contact surfaces. cleaner/sanitizer.
1.2 This test method may also be used to evaluate the
4. Apparatus
antimicrobial efficacy of one-step cleaner/sanitizer formula-
4.1 Balance—Abalance with a platform to accommodate a
tions recommended for use on lightly soiled, inanimate,
nonporous, non-food contact surfaces. 100-mL volumetric flask. This balance should be sensitive to
0.01 g.
1.3 It is the responsibility of the investigator to determine
whether Good Laboratory Practices (GLP) is required and to 4.2 Nonporous Test Surfaces, pre-cleaned.
4.2.1 Borosilicate Glass Squares, 25 by 25 by 2 mm slides,
follow them where appropriate (see section 40 CFR, 160) or as
revised. nonchipped.
4.2.2 Glazed Glass or Stainless Steel, of appropriate type,
1.4 This standard may involve hazardous materials, chemi-
cals and microorganisms and should be performed only by approximately same size as in 4.2.1.
4.3 Glass Culture Tubes, recommended sizes: 18 to 20 by
persons who have had formal microbiological training. This
standard does not purport to address all of the safety concerns, 150 mm and 25 by 150 mm without lip.
4.4 Culture Tube Closures, appropriate sized nontoxic clo-
if any, associated with its use. It is the responsibility of the user
of this standard to establish appropriate safety and health sures.
4.5 Pipets or Dispensing Syringes, (or both), appropriately
practices and determine the applicability of regulatory limita-
calibrated and sterile.
tions prior to use.
4.6 Bacteriological Transfer Loop, 4 mm inside diameter
2. Referenced Documents
loop of platinum or platinum alloy wire or sterile, disposable
2
2.1 ASTM Standards: plastic loops of approximate size.
D1193 Specification for Reagent Water 4.7 Flasks or Containers:
E1054 Test Methods for Evaluation of Inactivators of An- 4.7.1 Appropriate sizes with closures for preparation of
timicrobial Agents culture medium and sterile distilled water.
2.2 Federal Standard: 4.7.2 Volumetric, 100 and 1000 mL, sterile.
3
40 CFR, Part 160, Good Laboratory Practice Standards 4.8 Petri dishes, recommended sizes: 50 by 9 mm plastic,
and 100 by 15 mm, glass and plastic; sterile.
4.9 Jars, ointment jars, 2 oz (60 mL) with nontoxic lids,
sterile.
1
This test method is under the jurisdiction of ASTM Committee E35 on
4.10 Graduated Cylinders, recommended sizes; 100 and
Pesticides and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
500 mL.
Current edition approved April 10, 2003. Published July 2003. Originally
4.11 Flaming Apparatus—A bunsen burner or other appro-
approved 1987. Last previous edition approved in 1994 as E1153 – 94. DOI:
priate heat sterilizer.
10.1520/E1153-03.
2
4.12 Mixer—A “vortex” mixer is recommended.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
4.13 Timer—A reliable stopwatch or laboratory timer ca-
Standards volume information, refer to the standard’s Document Summary page on
pable of measuring elapsed time in seconds and minutes.
the ASTM website.
3
4.14 pH Meter—A reliable pH meter to determine pH of
Available from the Superintendent of Documents, U.S. Government Printing
Office, Washington, DC 20402. culture media.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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E1153 – 03
4.15 Desiccator, recommended size: 200 mm inside diam- Analysis may be performed by the method described in
eter with approximately 125-mm chamber depth from inside footnote 6(c) or by commercial available kit.
plate to cover flange, glass. 5.2.5 Allwaterusedforpreparationoftestsolutionsshallbe
4.16 Incubator, capable of maintaining temperature of 37 6 sterile.
2°C. 5.3 Sanitizing Solutions—Freshly prepared solutions of
4.17 Sterilizer, steam sterilizer and hot air oven (180 6 2°C sanitizers shall be used in all tests.
for 2 h). 5.4 Neutralizing Solutions—Solutions appropriate to inacti-
4.18 Co
...