ASTM E2362-04

Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection

SIGNIFICANCE AND USE
This test method may be used to determine if a pre-saturated or impregnated towelette demonstrates antimicrobial effectiveness as a disinfectant on hard surfaces.
SCOPE
1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a hard surface disinfectant.
1.2 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLPs) are required and to follow them when appropriate.
1.3 This practice should be performed only by those trained in microbiological techniques.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Sep-2004
• ICS: 71.100.35 - Chemicals for industrial and domestic disinfection purposes
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Relations

• Replaced By: ASTM E2362-09 - Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection
• Effective Date: 01-Dec-2009
• Referred By: ASTM E2967-15 - Standard Test Method for Assessing the Ability of Pre-wetted Towelettes to Remove and Transfer Bacterial Contamination on Hard, Non-Porous Environmental Surfaces Using the Wiperator
• Effective Date: 01-Oct-2004

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information.
Designation:E2362–04
Standard Practice for
Evaluation of Pre-saturated or Impregnated Towelettes for
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Hard Surface Disinfection
This standard is issued under the fixed designation E2362; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.5 neutralizer, n—a component used to render an active
agent incapable of destroying organisms by chemical or
1.1 This practice is designed to evaluate the antimicrobial
physical means.
activity of pre-saturated or impregnated towelettes when used
3.6 pre-saturated, adj—to be filled or impregnated with test
as a hard surface disinfectant.
substance prior to the time of its intended use.
1.2 It is the responsibility of the investigator to determine
3.7 towelette, n—A paper, cloth or non –woven blend
whether Good Laboratory Practices (GLP’s) are required and
material used as a transporter for a cleaning and/or disinfection
to follow them when appropriate.
agent.
1.3 This practice should be performed only by those trained
in microbiological techniques.
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4. Summary of Practice
1.4 This standard does not purport to address all of the
4.1 A towelette impregnated or pre-saturated with a test
safety concerns, if any, associated with its use. It is the
substance is used to treat a carrier which has been inoculated
responsibility of the user of this standard to establish appro-
withatestorganismafteranaliquotofatestorganismhasbeen
priate safety and health practices and to determine the
inoculated, evenly distributed, and dried onto the carrier. The
applicability of regulatory limitations prior to use.
carrier is wiped using the pre-saturated or impregnated tow-
2. Referenced Documents elette simulating the application of the test substance and then
2
held for a pre-determined contact time. After the specified
2.1 ASTM Standards:
contact time, the test substance remaining on the carrier is
D1193 Specification for Reagent Water
neutralized and the carrier is subcultured to recover surviving
E1054 Test Methods for Evaluation of Inactivators of An-
testorganism.Theusedtowelette,afterthecontacttime,isalso
timicrobial Agents
cultured for surviving test organism.
2.2 Federal Standard
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40 CFR, Part 160, Good Laboratory Practice Standards
5. Significance and Use
3. Terminology This test method may be used to determine if a pre-saturated
or impregnated towelette demonstrates antimicrobial effective-
3.1 carrier, n—a transportable surface onto which a test
ness as a disinfectant on hard surfaces.
organism will be inoculated and dried. The carrier will be
treated with the test substance and subcultured for survivors.
6. Apparatus
3.2 CFU, n—colony forming units
6.1 Incubator—any calibrated incubator that maintains a
3.3 disinfectant, n—a physical or chemical agent or process
temperature specific for propagation of organisms. (for ex-
that destroys pathogenic or potentially pathogenic microorgan-
ample, bacteria and mycobacteria at 35 6 2 °C and fungi at
isms in/on surfaces or objects.
25 6 2 °C).
3.4 impregnated, adj—saturated with test substance.
6.2 Sterilizer—any suitable steam sterilizer that produces
the conditions of sterilization is acceptable.
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This practice is under the jurisdiction ofASTM Committee E35 on Pesticides
6.3 Test Towelettes—with instructions for use.
and Alternative Control Agents and is the direct responsibility of Subcommitee
E35.15 on Antimicrobial and Antiviral Agents. Current edition approved Oct. 1,
2004. Published October 2004. DOI: 10.1520/E2362-04.
2 4
For referenced ASTM standards, visit the ASTM website, www.astm.org, or United States Environmental Protection Agency, Efficacy Data Requirements,
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM “Pre-Saturated or Impregnated Towelettes for Hard Surface Disinfection” Standard
Standards volume information, refer to the standard’s Document Summary page on Operating Procedure for Testing of Towelette Disinfectants against Salmonella
the ASTM website. choleraesuis , Staphylococcus aureus, Pseudomonas aeruginosa, and Mycobacte-
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Available from the Superintendent of Documents, U.S. Government Printing rium bovis (BCG), EPA/OPP Microbiology Laboratory, Ft. Meade, MD. SOP#
Office, Washington D.C. 20402 MB09-01, Revised 11/08/00.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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E2362–04
6.4 Timer (Stop-clock)—a calibrated timer that displays min 7.5.2 Middlebrook 7H11 Agar—Mycobacteria.
and s. 7.5.3 Sabouraud Dextrose Agar—Fungi.
6.5 Spectrophotometer—calibr
...