ASTM E787-81(2001)e1
(Specification)Standard Specification for Disposable Glass Micro Blood Collection Pipets
Standard Specification for Disposable Glass Micro Blood Collection Pipets
SCOPE
1.1 This specification covers two dimensionally different disposable glass micropipets used primarily to collect whole human blood specimens for clinical analysis and testing. They are available as coated with heparin or uncoated.
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e1
Designation: E 787 – 81 (Reapproved 2001)
Standard Specification for
Disposable Glass Micro Blood Collection Pipets
This standard is issued under the fixed designation E 787; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
e NOTE—Keywords were added editorially October 2001.
1. Scope 5.2 Heparin—shall be the ammonium salt isolated from the
lungs or intestinal mucosa of beef or pork origin. The heparin
1.1 This specification covers two dimensionally different
potency shall be 1 mg of ammonium heparin compound which
disposable glass micropipets used primarily to collect whole
is equal to at least 100 USP units.
human blood specimens for clinical analysis and testing. They
are available as coated with heparin or uncoated.
6. Physical Requirements
2. Referenced Documents 6.1 Design—The disposable glass micro blood collection
pipets, both short and long, shall be straight and pulled to a
2.1 ASTM Standards:
2 tapered point at one end.Any cross section of the pipets, taken
E 438 Specification for Glasses in Laboratory Apparatus
in a plane perpendicular to the longitudinal axis, shall be
3. Terminology circular. The pipets shall be lightly firepolished at both ends
with no run-in and possess color bands to denote presence or
3.1 Definitions of Terms Specific to This Standard:
absence of heparin content.
3.1.1 disposable micropipets—in accordance with this
6.2 Dimensions:
specification and the expected product performance expressed
6.2.1 The short Caraway pipet shall be approximately 75
in this standard, those pipets which are to be used one time
mm long and 4 mm in outside diameter. The pipet shall hold a
only. Any institution or individual who reuses a disposable
liquid volume of 310 to 470 µL. The tapered point length and
pipet must bear full responsibility for its safety and effective-
tip orifice opening shall be as specified in Fig. 1.
ness.
6.2.2 The long Natelson pipet shall be approximately 150
4. Classification
mm long and 3 mm in outside diameter. The pipet shall hold a
liquid volume of 220 to 420 µL. The tapered point length and
4.1 This specification covers two dimensionally different
tip orifice opening shall be as specified in Fig. 2.
disposable glass pipets as follows:
6.3 Workmanship—The pipets, as illustrated in Fig. 1 and
4.1.1 Short Pipet—Approximately 75 mm long and coated
Fig. 2, shall be free of defects that noticeably detract from their
with heparin (Type I) or uncoated (Type II). These are
appearance or impair their serviceability. They shall be free of
commercially recognized as Caraway pipets.
lint, or significant foreign matter, loose or embedded when
4.1.2 Long Pipet—Approximately 150 mm long and coated
viewedundernormalroomlighting.Thetopandtipendsofthe
with heparin (Type I) or uncoated (Type II). These are
pipets shall be cut at approximately 90° to the pipet axis and
commercially recognized as Natelson pipets.
shall not be cracked or have jagged ends or chips that enter the
5. Materials and Manufacture
bore of the pipet.
6.4 Color Coding—Each disposable glass micro blood col-
5.1 Glass—The pipets shall be fabricated from borosilicate
lection pipet shall be color-coded to identify the pipet. The
glass, Type I, Class B, or soda lime glass, Type II, in
heparin-coated pipet (Type 1) shall have a red color band. The
accordance with Specification E 438.
uncoated pipet (Type 2) shall have a blue color band. The
location of these color bands shall be as specified in Fig. 1 and
This specification is under the jurisdiction of ASTM Committee E41 on
Fig. 2.
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
6.5 Capillary—The pipets, both short and long, shall be
Apparatus.
Current edition approved Feb. 27, 1981. Published April 1981. capable of drawing sheep plasma or human whole blood the
Annual Book of ASTM Standards, Vol 14.02.
full length of the pipet when tested as specified in 7.1.
Caraway, W. T., and Fanger, H., “Ultramicro Procedures In Clinical Chemis-
try,” American Journal of Clinical Pathology, 25, 1955, pp. 316–331.
Natelson, S., Ph.D., Micro-Techniques of Clinical Chemistry, Charles C.
Thomas, Springfield, Ill., 1961, p. 70. The United States Pharmacopeia, 19th Revision, pp. 229–230.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E 787
7.3.1.1 Prepare sheep plasma in accordance with the USP
assayforsodiumheparin.Add10mLofpreparedsheepplasma
to 2.0 mL of the 1.0 % calcium chloride solution used in the
heparin assay. Mix the sheep plasma and calcium chloride
solution well.
7.3.2 Preparation of Controls—Use samples of both the
plain sheep plasma and recalcified sheep plasma as controls in
accordance with the following:
7.3.2.1 Positive Control—Fill an uncoated (that is, nonhe-
parinized) pipet with recalcified sheep plasma.
7.3.2.2 Negative Control—Fill a coated (that is, heparin-
ized) pipet with plain sheep plasma.
Capacity: 310 to 470 µL
Coding: Red band-heparin-coated (Type I)
7.3.3 Procedure—Immediately after the preparation of re-
Blue band-uncoated (Type II)
calcified sheep plasma, fill the pipets by immersing the tips in
Dimensions in millimetres
the recalcified sheep plasma while holding the pipets near the
A Overall length 73–77
horizontal level to facilitate quick filling. Rock the pipet
B Outside diameter 3.90–4.20
C Inside diameter 2.40–2.80
several times to assure intimate mixing of plasma with heparin
D Inside tip diameter 0.70–1.50
on inner surface of capillary tube. Place the pipets in a
E Length of taper 6–12
horizontal position. At the end of 1 h, inspect the pipets
F Color band location 0–10
containing plasma for evidence of coag
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