ASTM E2646-08

Standard Specification for Amblyseius cucumeris (=Neoseiulus) Oudemans (Acarina: Phytoseiidae) (Withdrawn 2017)

SCOPE
1.1 This specification includes standard terminology, classification, and referenced documents as well as description of the test methods for determining the number of Amblyseius cucumeris Oudemans supplied on the bulk carrier or in slow release sachets. Description of the method for assessing the purity of shipments is also included.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
WITHDRAWN RATIONALE
This specification includes standard terminology, classification, and referenced documents as well as description of the test methods for determining the number of Amblyseius cucumeris Oudemans supplied on the bulk carrier or in slow release sachets. Description of the method for assessing the purity of shipments is also included.
Formerly under the jurisdiction of Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents, this specification was withdrawn in January 2017 in accordance with section 10.6.3 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

General Information

• Status: Withdrawn
• Publication Date: 30-Sep-2008
• Withdrawal Date: 08-Jan-2017
• ICS: 65.100.01 - Pesticides and other agrochemicals in general
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E2646 −08
Standard Specification for
Amblyseius cucumeris (= Neoseiulus) Oudemans (Acarina:
Phytoseiidae)
This standard is issued under the fixed designation E2646; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4.3 Order—Acarina.
1.1 This specification includes standard terminology, 4.4 Family—Phytoseiidae.
classification, and referenced documents as well as description
4.5 Genus—Amblyseius = Neoseiulus.
of the test methods for determining the number of Amblyseius
4.6 Species—cucumeris.
cucumeris Oudemans supplied on the bulk carrier or in slow
release sachets. Description of the method for assessing the
5. Summary of Test Method (Determining the Number of
purity of shipments is also included.
N. cucumeris Released from a Slow Release Sachet)
1.2 The values stated in SI units are to be regarded as
5.1 The test describes methods for (1) estimation of the
standard. No other units of measurement are included in this
number of A. cucumeris released from sachet based on exam-
standard.
ining a few randomly selected sachets per shipment, and (2)
assessing the purity of shipment.
2. Referenced Documents
2 5.2 Afewsachetsarechosenrandomlyfromashipmentand
2.1 ASTM Standards:
each sachet is suspended from a wire hanger and placed on a
E2200Specification for Information Included with Packag-
sticky trap surface (“Catch it,” Silvandersson Sweden AB).
ing of Multi-Cellular Biological Control Organisms
Mites are allowed to disperse from sachets, and each week,
(Withdrawn 2010)
mites caught on the sticky trap are counted and recorded.
3. Terminology
6. Significance and Use
3.1 name of product—Amblyseius cucumeris Oudemans.
6.1 The biological control of flower thrips by N. cucumeris
3.2 preferred host prey—Western flower thrips (Franklin-
is, in part, dependent on accurate release rates and consistent
iella occidentalis).
release pattern of N. cucumeris. Supplying fewer predatory
3.3 onion thrips (Thrips tabaci)—also cyclamen and broad
mites than indicated on the label or restricting duration of
mites.
release may upset host-predator balance and could lead to the
failure of biological control. This test was developed for the
3.4 life stage when shipped—all stages, eggs, nymphs,
benefit of biocontrol producers and IPM practitioners.
adults.
7. Materials
4. Classification
7.1 Yellowstickytrapswithagridandcoatedwithdryglue.
4.1 Phylum—Arthropoda.
7.2 A 20-cm high wire hanger.
4.2 Class—Arachnida.
8. Test Unit
This specification is under the jurisdiction of ASTM Committee E35 on
8.1 A shipment of A. cucumeris is considered a test unit.
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
responsibility of Subcommittee E35.30 on Natural Multi-Cellular (Metazoan)
9. Pre-Test Conditions
Biological Control Organisms.
Current edition approved Oct. 1, 2008. Published October 2008. DOI: 10.1520/
9.1 Samples could be held in a cool place at 10 to 20°C out
E2646-08.
of direct sunlight for a maximum of 24 h before testing.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on 10. Sample Size
the ASTM website.
10.1 Choosefourormoresachetsfromashipment(needsto
The last approved version of this historical standard is referenced on
www.astm.org. be determined).
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E2646−08
11. Testing and Calculations
11.1 Suspend each sachet on a wire hanger and place on a
stickytrapsurface,onepertrap(Fig.1).Laboratoryevaluation
should be carried out under 16:8 D:N photoperiod 18 to 21°C
and75to80%RH(relativehumidity).Whentestingiscarried
out in the greenhouse, place the sticky traps and sachet inside
a small cage (45 by 30 by 27 cm) made of a white breathable
fabric(Fig.2).Positionthecagewithinthecropbutawayfrom
the heating pipes.
11.2 Allow mites to disperse until from the sachet until no
moremitesarereleased;thatis,sixormoreweeks.Changethe
sticky traps weekly and count and record the number of N.
cucumeris caught on the trap. Calculate the average number of
the predatory mites released per sachet
12. Results
FIG. 2Light Fabric Cage Containing Sticky Trap with Sachet Sus-
pended from Wire Hanger
12.1 The average number of A. cucumeris released per
sachet should be equal to or greater than the number specified
on the package.
15.6 Three sieves made from 150-mL wide mouth plastic
13. Summary of Test Method (Determining the Purity
jars the following mesh sizes:
and Number of Live Adults and Nymphs in 1.5-L
15.6.1 Top—Mesh pore size = 1200 microns (standard
Containers with Amblyseius cucumeris)
window screen).
15.6.2 Middle—Mesh pore size = 700 microns.
13.1 This test describes a method of estimating the number
15.6.3 Bottom—Mesh pore size = 75 microns.
of A. cucumeris in a standard 1.5-L package with bran carrier,
based on a random sampling and counts of live nymph and
15.7 0.5%dish-washingdetergentsolutioninawashbottle.
adult predators.
15.8 9-cm diameter petri dishes.
14. Significance and Use 15.9 Mite-counting grid attached to a 9-cm petri dish
...