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ASTM

ASTM E934-94(2009)

(Specification)

Standard Specification for Serological Pipet, Disposable Plastic

Standard Specification for Serological Pipet, Disposable Plastic

ABSTRACT
This specification covers disposable plastic serological pipets, calibrated to deliver when measuring volumes of liquids. The pipets shall be fabricated from crystal-grade, uncolored polystyrene, or its regrind. They shall adhere to the property requirements discussed herein for design (shape, delivery tips, top end, dimensions, and outflow times), graduation and identification markings, capacity, and accuracy and coefficient of variation.
SCOPE
1.1 This specification covers disposable plastic serological pipets, calibrated “to deliver” when measuring volumes of liquids.
1.1.1 Any institution or individual who reuses a disposable pipet must bear full responsibility for its safety and effectiveness.

General Information

Status
Historical
Publication Date
31-Oct-2009
ICS
11.100 - Laboratory medicine
Technical Committee
E41 - Laboratory Apparatus
Drafting Committee
E41.01 - Laboratory Ware and Supplies
Current Stage
Ref Project

Relations

Replaces
ASTM E934-94(2004) - Standard Specification for Serological Pipet, Disposable Plastic
Effective Date
01-Nov-2009

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ASTM E934-94(2009) - Standard Specification for Serological Pipet, Disposable PlasticASTM E934-94(2009) - Standard Specification for Serological Pipet, Disposable Plastic
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ASTM E934-94(2009) - Standard Specification for Serological Pipet, Disposable Plastic
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E934 −94(Reapproved2009)
Standard Specification for
Serological Pipet, Disposable Plastic
This standard is issued under the fixed designation E934; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope 4. Material and Manufacturer
1.1 This specification covers disposable plastic serological 4.1 The pipets made to this specification shall be fabricated
pipets, calibrated “to deliver” when measuring volumes of from crystal grade, uncolored polystyrene, or regrind of same,
liquids. in accordance with Specification D703.
1.1.1 Any institution or individual who reuses a disposable
pipet must bear full responsibility for its safety and effective- 5. Design
ness.
5.1 Shape—0.5,1.0,and2.0-cm pipetsshallbestraightand
of one-piece construction.Any cross section of a pipet taken in
2. Referenced Documents
a plane perpendicular to the longitudinal axis shall be circular.
2.1 ASTM Standards:
5.1.1 Pipetsof5.0,10.0,25.0,and50.0cm shallbestraight
D703Specification for Polystyrene Molding and Extrusion
andmayconsistofone,two,orthreecomponents,theextruded
Materials (Withdrawn 0)
pipet barrel, the pulled or injection molded tip, and the plain,
E542Practice for Calibration of Laboratory Volumetric
pulled, or injection molded top end. Any cross section of a
Apparatus
pipet taken in a plane perpendicular to the longitudinal axis
E920Specification for Commercially Packaged Laboratory
shall be circular.
Apparatus
5.2 Delivery Tips—Delivery tips shall be made with a
E921Specification for Export Packaged Laboratory Appa-
gradual taper of 10 to 40 mm. The tip end shall be reasonably
ratus
perpendicular to the longitudinal axis of the pipet, and shall be
E1133Practice for Performance Testing of Packaged Labo-
free of internal flash.
ratory Apparatus for United States Government Procure-
ments 5.3 TopEnd—The5,10,25.0,and50.0-cm sizesshallhave
a top end with an inside diameter of 2 to 6.5 mm for a
3. Terminology
minimumdistanceof20mmfromtheopenend,andshallhave
anoveralllengthof24to28mm.Onallsizes,thetopendshall
3.1 Definitions of Terms Specific to This Standard:
besuitableforpluggingwithfilteringmaterial.Thetopmaybe
3.1.1 accuracy—the expected distribution of mean volumes
flat or chamfered and must be without sharp outer edges. All
around the stated volume.
top ends shall be reasonably perpendicular to the longitudinal
3.1.2 coeffıcient of variation—the expected distribution of
axis of the pipet. The O.D. of top end should range from 7 to
individual volumes around the mean volume.
9 mm.
3.1.3 disposable pipet—such pipets will only be expected to
5.4 Dimensions and Outflow Times—The limiting dimen-
providetheirspecifiedperformanceduringtheiroriginaluseor
sions and outflow times shall be as shown in Table 1. Outflow
operation.
times shall be determined by means of a stopwatch on
unplugged pipets when using distilled water at 25 6 5°C.
This specification is under the jurisdiction of ASTM Committee E41 on
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
6. Markings
Apparatus.
Current edition approved Nov. 1, 2009. Published December 2009. Originally
6.1 Graduation Markings—Graduation lines shall be be-
approved in 1983. Last previous edition approved in 2004 as E934–94(2004).
tween 0.2 mm and 0.5 mm in width, and in a plane perpen-
DOI: 10.1520/E0934-94R09.
dicular to the longitudinal axis of the pipet parallel to each
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
other.Amaingraduationlineshallextendatleast ⁄5oftheway
Standards volume information, refer to the standard’s Document Summary page on
around the pipet. The values of all main graduation lines shall
the ASTM website.
be in Arabic numbers directly above the lines referenced.
The last approved version of this historical standard is referenced on
www.astm.org. Intermediategraduationlinesshallextendatleast ⁄5oftheway
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E934−94(2009)
TABLE 1 Dimensions and Outflow Times
Least Value Graduation cm (mL) Outflow, s
Outside Diameter W
...

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