ASTM D7574-23

Standard Test Method for Determination of Bisphenol A in Environmental Waters by Liquid Chromatography/Tandem Mass Spectrometry

SIGNIFICANCE AND USE
5.1 The first reported synthesis of BPA was by the reaction of phenol with acetone by Zincke.4 BPA has become an important high volume industrial chemical used in the manufacture of polycarbonate plastic and epoxy resins. Polycarbonate plastic and resins are used in numerous products including electrical and electronic equipment, automobiles, sports and safety equipment, reusable food and drink containers, electrical laminates for printed circuit boards, composites, paints, adhesives, dental sealants, protective coatings and many other products.5  
5.2 The environmental source of BPA is predominantly from the decomposition of polycarbonate plastics and resins. BPA is not classified as bio-accumulative by the U.S. Environmental Protection Agency and will biodegrade. BPA has been reported to have adverse effects in aquatic organisms and may be released into environmental waters directly at trace levels through landfill leachate and POTW effluents. This method has been investigated for use with surface water and secondary and tertiary POTW effluent samples therefore, it is applicable to these matrices only. It has not been investigated for use with salt water or solid sample matrices.
SCOPE
1.1 This test method covers the determination of bisphenol A (BPA) extracted from water utilizing solid phase extraction (SPE), separated using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS). BPA is qualitatively and quantitatively determined by this method. This test method adheres to multiple reaction monitoring (MRM) mass spectrometry.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 The method detection limit (MDL) and reporting limit (RL) for BPA are listed in Table 1.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 14-Apr-2023
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: D19 - Water
• Drafting Committee: D19.06 - Methods for Analysis for Organic Substances in Water

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D7574 − 23
Standard Test Method for
Determination of Bisphenol A in Environmental Waters by
1
Liquid Chromatography/Tandem Mass Spectrometry
This standard is issued under the fixed designation D7574; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope D3694 Practices for Preparation of Sample Containers and
for Preservation of Organic Constituents
1.1 This test method covers the determination of bisphenol
D3856 Guide for Management Systems in Laboratories
A (BPA) extracted from water utilizing solid phase extraction
Engaged in Analysis of Water
(SPE), separated using liquid chromatography (LC) and de-
D5847 Practice for Writing Quality Control Specifications
tected with tandem mass spectrometry (MS/MS). BPA is
for Standard Test Methods for Water Analysis
qualitatively and quantitatively determined by this method.
D5905 Practice for the Preparation of Substitute Wastewater
This test method adheres to multiple reaction monitoring
3
2.2 Other Documents:
(MRM) mass spectrometry.
40 CFR Part 136, Appendix B Definition and Procedure for
1.2 The values stated in SI units are to be regarded as
the Determination of the Method Detection Limit
standard. No other units of measurement are included in this
standard.
3. Terminology
1.3 The method detection limit (MDL) and reporting limit
3.1 Definitions:
(RL) for BPA are listed in Table 1.
3.1.1 For definitions of terms used in this standard, refer to
1.4 This standard does not purport to address all of the
Terminology D1129.
safety concerns, if any, associated with its use. It is the
3.2 Definitions of Terms Specific to This Standard:
responsibility of the user of this standard to establish appro-
3.2.1 environmental water, n—shall refer to water tested
priate safety, health, and environmental practices and deter-
using this method. See Section 5.
mine the applicability of regulatory limitations prior to use.
3.2.2 independent reference material, IRM, n—a material of
1.5 This international standard was developed in accor-
known purity and concentration obtained either from the
dance with internationally recognized principles on standard-
National Institute of Standards and Technology (NIST) or other
ization established in the Decision on Principles for the
reputable supplier; the IRM must be obtained from a different
Development of International Standards, Guides and Recom-
lot of material than is used for calibration.
mendations issued by the World Trade Organization Technical
3.3 Acronyms:
Barriers to Trade (TBT) Committee.
3.3.1 CCC, n—Continuing Calibration Check
2. Referenced Documents 3.3.2 IC, n—Initial Calibration
2
3.3.3 LC, n—Liquid Chromatography
2.1 ASTM Standards:
D1129 Terminology Relating to Water
3.3.4 LCS/LCSD, n—Laboratory Control Sample/
D1193 Specification for Reagent Water
Laboratory Control Sample Duplicate
D2777 Practice for Determination of Precision and Bias of
3.3.5 MDL, n—Method Detection Limit
Applicable Test Methods of Committee D19 on Water
3.3.6 MeOH, n—Methanol
-3
3.3.7 mM, n—millimolar, 1 × 10 moles/L
1
This test method is under the jurisdiction of ASTM Committee D19 on Water 3.3.8 MRM, n—Multiple Reaction Monitoring
and is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
3.3.9 MS/MSD, n—Matrix Spike/Matrix Spike Duplicate
Organic Substances in Water.
Current edition approved April 15, 2023. Published June 2023. Originally
3.3.10 NA, adj—Not Available
approved in 2009. Last previous edition approved in 2016 as D7574 – 16. DOI:
10.1520/D7574-23.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Available from U.S. Government Printing Office Superintendent of Documents,
Standards volume information, refer to the standard’s Document Summary page on 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
the ASTM website. www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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D7574 − 23
TABLE 1 MDL and Reporting Range
laminates for printed circuit boards, composites, paints,
A B
Analyte MDL (ng/L) Reporting Range (ng/L) adhesives, dental sealants, protective coatings and many other
5
Bisphenol A 5 20–600
products.
A
MDL determined following the Code of Federal Regulations, 40 CFR Part 136,
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D7574 − 16 D7574 − 23
Standard Test Method for
Determination of Bisphenol A in Environmental Waters by
1
Liquid Chromatography/Tandem Mass Spectrometry
This standard is issued under the fixed designation D7574; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This procedure test method covers the determination of bisphenol A (BPA) extracted from water utilizing solid phase
extraction (SPE), separated using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS). BPA is
qualitatively and quantitatively determined by this method. This test method adheres to multiple reaction monitoring (MRM) mass
spectrometry.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 The method detection limit (MDL) and reporting limit (RL) for BPA are listed in Table 1.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and healthsafety, health, and environmental practices and determine
the applicability of regulatory limitations prior to use.
1.5 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water
D3694 Practices for Preparation of Sample Containers and for Preservation of Organic Constituents
D3856 Guide for Management Systems in Laboratories Engaged in Analysis of Water
D5847 Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
D5905 Practice for the Preparation of Substitute Wastewater
3
2.2 Other Documents:
The Code of Federal Regulations40 CFR Part 136, Appendix B 40 CFR Part 136, Appendix BDefinition and Procedure for the
Determination of the Method Detection Limit
1
This test method is under the jurisdiction of ASTM Committee D19 on Water and is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
Organic Substances in Water.
Current edition approved Feb. 1, 2016April 15, 2023. Published March 2016June 2023. Originally approved in 2009. Last previous edition approved in 20092016 as
D7574D7574 – 16. – 09. DOI: 10.1520/D7574-16.DOI: 10.1520/D7574-23.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7574 − 23
TABLE 1 MDL and Reporting Range
A B
Analyte MDL (ng/L) Reporting Range (ng/L)
Bisphenol A 5 20–600
A
MDL determined following the Code of Federal Regulations, 40CFR 40 CFR Part
136, Appendix B.
B
Lowest point of the reporting range, reporting limit, is calculated from the LV 1
concentration calibration standard in Table 4. Fig. 1 displays the signal/noise ratio
at the reporting limit.
3. Terminology
3.1 Definitions:
3.1.1 For definitions of terms used in this standard, refer to Terminology D1129.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 environmental water, n—shall refer to water tested using this method. See Section 5.
3.2.2 independent reference material, IRM, n—a material of known purity and concentration obtained either from the National
Institute of Standards and Technology (NIST) or other reputable supplier. The IRM shallsupplier; the IRM must be obtained from
a different lot of material than is used for calibration.
3.3 Acronyms:
3.3.1 CCC, n—Continuing Calibration Check
3.3.
...