ASTM E733-80(1999)e1

Standard Specification for 44.7-[mu]L Disposable Glass Micropipets (Withdrawn 2005)

SCOPE
1.1 This specification describes two different types of disposable micropipets, calibrated "to contain," used in measuring microlitre volumes of liquids.  
1.2 The following precautionary statement pertains only to the test method portion, Section 9, of this specification: This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
WITHDRAWN RATIONALE
This specification describes two different types of disposable micropipets, calibrated "to contain," used in measuring microlitre volumes of liquids.
Formerly under the jurisdiction of Committee E41 on Laboratory Apparatus, this specification was discontinued May 2005 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

General Information

• Status: Withdrawn
• Publication Date: 09-Dec-1999
• Withdrawal Date: 02-Nov-2005
• ICS: 11.100 - Laboratory medicine
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
e1
Designation: E 733 – 80 (Reapproved 1999)
Standard Specification for
44.7-µL Disposable Glass Micropipets
This standard is issued under the fixed designation E 733; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
e NOTE—Keywords were added in March 2000.
1. Scope 5. Material
1.1 This specification describes two different types of dis- 5.1 Glass—The pipets made to this specification shall be
posablemicropipets,calibrated“tocontain,”usedinmeasuring fabricated from borosilicate glass, Type I, Class A or B, or
microlitre volumes of liquids. soda-lime glass, Type II, in accordance with Specification
1.2 The following precautionary statement pertains only to E 438.
the test method portion, Section 9, of this specification: This 5.2 Heparin—Heparin shall be of sodium salt isolated from
standard does not purport to address all of the safety concerns, the intestinal mucosa of hog origin. The heparin potency shall
if any, associated with its use. It is the responsibility of the user be 1 mg of sodium heparin compound and shall be equal to at
of this standard to establish appropriate safety and health least 100 United States Pharmacopeia (USP) units.
practices and determine the applicability of regulatory limita-
6. Physical Requirements
tions prior to use.
6.1 Design—Pipets shall be of one-piece construction for
2. Referenced Documents
shape, dimensions, and permissible variations. Any cross
2.1 ASTM Standards: section of the pipet, taken in a plane perpendicular to the
E 438 Specification for Glasses in Laboratory Apparatus longitudinal axis, shall be circular. The pipet design is similar
E 672 Specification for Disposable Glass Micropipets to that expressed in Specification E 672 except for sizing,
2.2 Other Standard: heparin coating, and specific color coding requirements.
USP XIX United States Pharmacopeia 6.2 Capacity—The pipet shall be calibrated “to contain”
(T.C.) and shall have a capacity of 44.7 µL. This shall be
3. Terminology
known as the “stated capacity,” V , in making subsequent
3.1 Definitions of Terms Specific to This Standard:
calculations. The expected deviation from the stated capacity
3.1.1 accuracy—the expected distribution of mean volumes shall be expressed as accuracy and coefficient of variation and
around the stated volume.
shall be expressed on the package label. The pipets shall be
3.1.2 coeffıcient of variation—the expected distribution of tested for capacity as specified in 9.1.
individual volumes around the mean volume.
6.2.1 Accuracy—(see 4.1)—The accuracy from stated vol-
3.1.3 disposable micropipets—in accordance with this ume shall be within 6 0.5 % and shall be determined as
specification and the expected product performance expressed
specified in 9.4.
in this standard, those micropipets which are to be used one 6.2.2 Coeffıcient of Variation (see section 3.1.2)—The co-
time only. Any institution or individual who reuses a dispos-
efficientofvariationfromstatedvolumeshallnotexceed1.0 %
able micropipet must bear full responsibility for its safety and and shall be determined as specified in 9.4.
effectiveness.
6.3 Capacity Mark—Pipets (Fig. 1) shall have a capacity
line that is calibrated “to contain” a volume of liquid at 20°C.
4. Classification
The capacity line shall be 0.3 to 0.5 mm wide and shall
4.1 This specification covers two different pipets as follows:
completely encircle the pipet in a plane perpendicular to its
Type I—Coated with heparin.
longitudinal axis.
Type II—Uncoated.
6.4 Heparin Coating (Type I, Heparinized, only)—The
inner surface of Type I pipets shall be evenly coated with
sodium heparin. At his option, the manufacturer may label
This specification is under the jurisdiction of ASTM Committee E-41 on
TypeI,heparinizedpipetswithastatementofexpectedunitsof
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
heparin activity. An expiration date on specified units of
Apparatus.
Current edition approved April 10, 1980. Published June 1980. heparinactivitymaybeclaimedbythemanufacturer.Thepipet
Annual Book of ASTM Standards, Vol 14.04.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E 733
FIG. 1 44.7-µL Disposable Glass Micropipets: Type I—Coated with Heparin, Type II—Uncoated
shall contain a minimum of 5 and a maximum of 10 units of 7.2 The calibration line and color codes on Type I and Type
heparin that shall be present when tested as specified in 9.5. IIpipetsshallbeappliedtotheglasspipetatlocationsspecified
The manufacturer may heparin coat the pipet only to the in Fig. 1. The calibration line shall be sufficiently deposited on
calibration line. In this instance, a minimum of 2 and a theglasstoenablethesettingofameniscus,andthecolorband
maximum of 5 units of heparin shall be present when tested as shall be sufficiently deposited on the glass to identify the pipet
specified in 9.5. as to its stated volume.
6.5 Lot or Control Number—A lot or control number shall
8. Reading and Setting the Meniscus
be indicated on the pipet container package.This lot, or control
number, shall be traceable to the origin of the heparin lot 8.1 Reading a Water Meniscus—For all pipets, the reading
is made on the lowest point of the meniscus. In order that the
purchase or mix, or both.
6.6 Identification Markings: lowest point may be observed, it is necessary to place a shade
of some dark material immediately below and behind the
6.6.1 Type I, Heparinized—The pipet shall be identified for
capacity with a purple color code marking on each pipet that is meniscus, which renders the profile of the meniscus dark and
2 to 6 mm wide. An additional green color band that is 1 to 2 clearly visible against a light background.
mm wide shall be located above the capacity color code 8.1.1 Setting a Water Meniscus—Setting of the meniscus
marking to identify the existence of heparin content in the shall be performed by one of the following methods.Wherever
pipet. The location of these color bands shall be as specified in practical, the meniscus should descend to the position of
Fig. 1. setting.
6.6.2 Type II, Uncoated—The pipet shall be identified for 8.1.1.1 Method A—The position of the lowest point of the
capacity with a purple color code marking on each pipet that is meniscus with reference to the graduation line is horizontally
tangent to the plane of the upper edge of the graduation line.
2 to 6 mm wide. An additional green color band that is 1 to 2
mm wide shall be located above the capacity color code The position of the meniscus is obtained by having the eye in
the same plane of the upper edge of the graduation line.
marking to identify the existence of heparin content in the
pipet. The location of these color bands shall be as specified in 8.1.1.2 Method B—The position of the lowest point of the
meniscus with reference to the graduation line is such that it is
Fig. 1
in the plane of the middle of the graduation line. This position
7. Workmanship
of the meniscus is obtained by making the setting in the center
7.1 The pipets shall be free of defects that will detract from oftheellipseformedbythegraduationlineonthefrontandthe
their appearance or may impair their serviceability. The pipets back of the tube as observed by having the eye slightly below
shall be free of significant foreign matter, loose or embedded the plane of the graduation line. The setting is accurate if, as
lint or chips that affect the bore, or stains when view
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