Standard Test Method for Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes (Withdrawn 2020)

SIGNIFICANCE AND USE
5.1 The glass petri plate provides a closed system for enumeration and easy application of a pre-saturated or impregnated antimicrobial towelette by an analyst.  
5.2 Inoculation of carriers (five 10 µL spots of microbial suspension) is conducted using a template and a positive displacement pipette, thereby ensuring a precise inoculum level and uniform distribution of inoculum.  
5.3 A single towelette is tested per carrier, thereby ensuring comparable treatment among carriers and eliminating the likelihood of cross-contamination between carriers.  
5.4 The circular motion of the product application (wipe outside to inside, lift towelette to invert and wipe inside to outside) is a relevant motion that ensures uniform coverage and contact of disinfectant with the inoculated surface.  
5.5 The addition of neutralizer to the treated plates ensures thorough neutralization at the end of the product’s contact time. This test method provides a procedure for performing neutralization verification to confirm that the microbicidal and/or microbistatic activity of a test substance has been brought to an undetectable level at the end of the contact time.  
5.6 The design of the test method minimizes any loss of viable organisms through carrier wash-off.  
5.7 This test method provides for optional use of an organic soil load as dictated by a product’s label claim.  
5.8 It is optional to adjust (either dilute or concentrate) the inoculum level to achieve desired control carrier counts and to accommodate different product performance standards.
SCOPE
1.1 This test method provides detailed instructions for performing a quantitative evaluation of antimicrobial efficacy of a towelette when challenged against Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica. The method may be used with other microbial strains, though modification may be necessary to accommodate recovery.  
1.1.1 Antimicrobial towelettes, designed to decontaminate hard, non-porous surfaces, are diverse in size, matrix composition, and packaging.  
1.1.2 Antimicrobial towelettes also vary in label claims and use directions.  
1.2 This quantitative method does not differentiate between mechanical removal of inoculum from a surface and chemical inactivation of the test microbe; rather, product efficacy is considered a combination of both attributes of a towelette-based formulation.  
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP Standards—40 CFR, Part 160 of FIFRA) are required and to follow them when appropriate.  
1.4 This standard may involve the use of hazardous materials, chemicals and infectious microorganisms and should be performed only by persons with formal training in microbiology.  
1.5 Strict adherence to the protocol is necessary for the validity of the test results.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard does not address specific product performance standards established by regulatory authorities; see Section 10, Note 2 for details.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
WITHDRAWN RATIONALE
This test method provides detailed instructions for performing a quantitative evaluation of antimicrobial efficacy of a towelette when challenged against Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica. The method may be used with other microbial strains, though modification may be necessary to accommodate recovery.
Formerly under the jurisdiction of Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents, this test method was withdrawn in October 2020. T...

General Information

Status
Withdrawn
Publication Date
14-Nov-2012
Withdrawal Date
04-Oct-2020
Current Stage
Ref Project

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ASTM E2896-12 - Standard Test Method for Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes (Withdrawn 2020)
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2896 − 12
Standard Test Method for
Quantitative Petri Plate Method (QPM) for Determining the
1
Effectiveness of Antimicrobial Towelettes
This standard is issued under the fixed designation E2896; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
This test method provides a standardized approach to quantitatively determine the effectiveness of
antimicrobial towelettes (wipes) in treating hard non-porous surfaces contaminated with Staphylo-
coccus aureus, Pseudomonas aeruginosa, and Salmonella enterica. This test method addresses the
2
need for a user-friendly, relevant, and reproducible procedure.
1. Scope 1.5 Strict adherence to the protocol is necessary for the
validity of the test results.
1.1 This test method provides detailed instructions for
performing a quantitative evaluation of antimicrobial efficacy 1.6 The values stated in SI units are to be regarded as
ofatowelettewhenchallengedagainst Staphylococcus aureus, standard. No other units of measurement are included in this
Pseudomonas aeruginosa and Salmonella enterica. The standard.
method may be used with other microbial strains, though
1.7 This standard does not address specific product perfor-
modification may be necessary to accommodate recovery.
mance standards established by regulatory authorities; see
1.1.1 Antimicrobial towelettes, designed to decontaminate
Section 10, Note 2 for details.
hard, non-porous surfaces, are diverse in size, matrix
1.8 This standard does not purport to address all of the
composition, and packaging.
safety concerns, if any, associated with its use. It is the
1.1.2 Antimicrobial towelettes also vary in label claims and
responsibility of the user of this standard to establish appro-
use directions.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
1.2 This quantitative method does not differentiate between
mechanical removal of inoculum from a surface and chemical
2. Referenced Documents
inactivation of the test microbe; rather, product efficacy is
considered a combination of both attributes of a towelette-
2.1 Other Documents:
based formulation.
AOAC Official Method 961.02,Germicidal Spray Products
3
as Disinfectants. Revised 2012
1.3 It is the responsibility of the investigator to determine
whetherGoodLaboratoryPractices(GLPStandards—40CFR, AOAC Official Method 955.15,Use-Dilution Method for
Testing Disinfectants against Staphylococcus aureus. Re-
Part 160 of FIFRA) are required and to follow them when
3
appropriate. vised 2012
AOAC Official Method 964.02,Use-Dilution Method for
1.4 This standard may involve the use of hazardous
Testing Disinfectants against Pseudomonas aeruginosa.
materials,chemicalsandinfectiousmicroorganismsandshould
3
Revised 2012
be performed only by persons with formal training in micro-
AOAC Official Methods 955.14,Use-Dilution Method for
biology.
Testing Disinfectants against Salmonella enterica. Re-
3
vised 2012
40CFR,Part160FederalInsecticide,FungicideandRoden-
1
4
This test method is under the jurisdiction of ASTM Committee E35 on
ticideAct(FIFRA);GoodLaboratoryPracticeStandards
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Nov. 15, 2012. Published December 2012. DOI:
3
10.1520/E2896-12. Available from AOAC International, 481 North Frederick Ave., Suite 500,
2
Samalot-Freire, L., Tomasino, S. F., and Hasan, J. A., The Quantitative Petri Gaithersburg, Maryland 20877-2417, http://www.aoac.org.
4
Plate Method (QPM): A New Method for Assessing the Efficacy of Antimicrobial AvailablefromU.S.GovernmentPrintingOfficeSuperintendentofDocuments,
Towelettes,Presentedatthe125thAnnualMeetingoftheAOACInternational,New 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
Orleans, LA, 2011. www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E2896 − 12
3. Terminology displacement pipette, thereby ensuring a precise inoculum
level and uniform distribution of inoculum.
3.1 Definitions:
3.1.1 antimicrobial towelette (wipe), n—a piece of porous 5.3 Asingle towelette is tested per carrier, thereby ensuring
material soaked in an antimicrobial liquid that is meant for comparable treatment among carriers and eliminating the
decontaminationofhardnon-porousenvironmentalsurfacesby likelihood of cross-contamination between carriers.
wiping.
5.4 The circula
...

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