EN ISO 20483:2013

Cereals and pulses - Determination of the nitrogen content and calculation of the crude protein content - Kjeldahl method (ISO 20483:2013)

ISO 20483:2013 specifies a method for the determination of the nitrogen content of cereals, pulses and derived products, according to the Kjeldahl method, and a method for calculating the crude protein content. The method does not distinguish between protein nitrogen and non-protein nitrogen.

Getreide und Hülsenfrüchte - Bestimmung des Stickstoffgehaltes und Berechnung des Rohproteingehaltes - Kjeldahl-Verfahren (ISO 20483:2013)

Diese Internationale Norm legt ein Verfahren zur Bestimmung des Stickstoffgehaltes von Getreide, Hülsen-früchten und deren Produkten nach dem Kjeldahl-Verfahren und ein Verfahren zur Berechnung des Rohproteingehaltes fest.
Das Verfahren unterscheidet nicht zwischen Proteinstickstoff und Nichtproteinstickstoff. Wenn es wichtig ist, den Gehalt an Nichtproteinstickstoff zu bestimmen, würde ein geeignetes Verfahren hierzu angewendet werden.
ANMERKUNG   In bestimmten Fällen ist eine vollständige Erfassung des in Nitraten und Nitriten vorliegenden Stickstoffs mit diesem Verfahren nicht möglich.

Céréales et légumineuses - Détermination de la teneur en azote et calcul de la teneur en protéines brutes - Méthode de Kjeldahl (ISO 20483:2013)

L'ISO 20483:2013 spécifie une méthode pour la détermination de la teneur en azote des céréales, des légumineuses et des produits dérivés, selon la méthode de Kjeldahl, ainsi qu'une méthode de calcul de la teneur en protéines brutes. La méthode ne fait pas la distinction entre l'azote protéique et l'azote non protéique.

Žito in stročnice - Določevanje dušika in izračun deleža surovih beljakovin - Metoda po Kjeldahlu (ISO 20483:2013)

General Information

• Status: Published
• Publication Date: 03-Dec-2013
• Withdrawal Date: 29-Jun-2014
• ICS: 67.060 - Cereals, pulses and derived products
• Technical Committee: CEN/TC 338 - Cereal and cereal products
• Drafting Committee: CEN/TC 338 - Cereal and cereal products
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 04-Dec-2013
• Completion Date: 04-Dec-2013
• Ref Project: SIST EN ISO 20483:2014 - Cereals and pulses - Determination of the nitrogen content and calculation of the crude protein content - Kjeldahl method (ISO 20483:2013)

Relations

• Replaces: EN ISO 20483:2006 - Cereals and pulses - Determination of the nitrogen content and calculation of the crude protein content - Kjeldahl method (ISO 20483:2006)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
01-februar-2014
1DGRPHãþD
SIST EN ISO 20483:2007
äLWRLQVWURþQLFH'RORþHYDQMHGXãLNDLQL]UDþXQGHOHåDVXURYLKEHOMDNRYLQ
0HWRGDSR.MHOGDKOX,62
Cereals and pulses - Determination of the nitrogen content and calculation of the crude
protein content - Kjeldahl method (ISO 20483:2013)
Getreide und Hülsenfrüchte - Bestimmung des Stickstoffgehaltes und Berechnung des
Rohproteingehaltes - Kjeldahl-Verfahren (ISO 20483:2013)
Céréales et légumineuses - Détermination de la teneur en azote et calcul de la teneur en
protéines brutes - Méthode de Kjeldahl (ISO 20483:2013)
Ta slovenski standard je istoveten z: EN ISO 20483:2013
ICS:
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 20483
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2013
ICS 67.060 Supersedes EN ISO 20483:2006
English Version
Cereals and pulses - Determination of the nitrogen content and
calculation of the crude protein content - Kjeldahl method (ISO
20483:2013)
Céréales et légumineuses - Détermination de la teneur en Getreide und Hülsenfrüchte - Bestimmung des
azote et calcul de la teneur en protéines brutes - Méthode Stickstoffgehaltes und Berechnung des Rohproteingehaltes
de Kjeldahl (ISO 20483:2013) - Kjeldahl-Verfahren (ISO 20483:2013)
This European Standard was approved by CEN on 7 September 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20483:2013 E
worldwide for CEN national Members.

Contents Page
Foreword .3
Foreword
This document (EN ISO 20483:2013) has been prepared by Technical Committee ISO/TC 34 “Food products”
in collaboration with Technical Committee CEN/TC 338 “Cereal and cereal products” the secretariat of which
is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2014, and conflicting national standards shall be withdrawn at
the latest by June 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 20483:2006.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 20483:2013 has been approved by CEN as EN ISO 20483:2013 without any modification.

INTERNATIONAL ISO
STANDARD 20483
Second edition
2013-12-01
Cereals and pulses — Determination
of the nitrogen content and calculation
of the crude protein content —
Kjeldahl method
Céréales et légumineuses — Détermination de la teneur en azote et
calcul de la teneur en protéines brutes — Méthode de Kjeldahl
Reference number
ISO 20483:2013(E)
©
ISO 2013
ISO 20483:2013(E)
© ISO 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2013 – All rights reserved

ISO 20483:2013(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 3
8 Preparation of test sample . 3
9 Determination of the moisture content . 4
10 Procedure. 4
10.1 General . 4
10.2 Test portion . 4
10.3 Determination . 4
10.4 Blank test . 5
10.5 Test with reference material (check test) . 5
11 Expression of results . 5
11.1 Nitrogen content . 5
11.2 Crude protein content . 6
12 Precision . 6
12.1 Interlaboratory test. 6
12.2 Repeatability . 6
12.3 Reproducibility . 6
12.4 Critical difference. 6
13 Test report . 7
Annex A (informative) Results of interlaboratory tests. 8
Annex B (informative) Critical difference and practical application of the repeatability and
reproducibility limits to different protein contents .10
Annex C (informative) Factors for converting nitrogen content to protein content .12
Bibliography .13
ISO 20483:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directives
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received. www.iso.org/patents
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
The committee responsible for this document is ISO/TC 34, food and food products, Subcommittee SC 4,
cereals and pulses.
This second edition cancels and replaces the first edition (ISO 20483:2006), which has been
technically revised.
iv © ISO 2013 – All rights reserved

INTERNATIONAL STANDARD ISO 20483:2013(E)
Cereals and pulses — Determination of the nitrogen
content and calculation of the crude protein content —
Kjeldahl method
WARNING — The use of this International Standard can involve hazardous materials, operations
and equipment. This International Standard does not purport to address all the safety problems
associated with its use. It is the responsibility of the user of this International Standard to
establish appropriate safety practices and determine the
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