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This document specifies a method for the analysis of total folate in infant formula and adult nutritionals using trienzyme extraction and ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).

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This document specifies a procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on acid catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC/MS analysis. This document is applicable to solid and liquid fats and oils. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

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This document specifies a method for the application of balanced incomplete block designs to sensory descriptive and hedonic tests. This document is applicable when the number of test samples exceeds the number of evaluations that an assessor can perform reliably in a single session. This document also specifies the fundamental characteristics of balanced incomplete block designs and establishes guidelines for their application in sensory evaluation.

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This document specifies requirements for bitter fennel seed (Foeniculum vulgare P. Miller var. vulgare), whole or ground.
Recommendations relating to storage and transport conditions are given in Annex A.
ISO 7927-2 specifies requirements for sweet fennel seed.
NOTE Although, botanically speaking, the product is a “fruit”, the term “seed” is in common commercial use.

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This document specifies a reference method for the determination of the nitrogen content of meat and meat products by the Kjeldahl principle.

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  • Standard
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This document specifies two reference methods for the determination of the moisture content of meat and meat products: a direct drying method and a distillation method.
The direct drying method is applicable to meat and meat products with low volatile substances in addition to moisture.
The distillation method is applicable to meat and meat products with high volatile substances in addition to moisture.

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This document specifies minimum performance requirements for methods that quantify the food allergens milk, egg, peanut, hazelnut, almond, brazil nut, macadamia nut, cashew, pistachio nut, walnut, pecan nut, lupine, sesame, mustard, soy, celery, fish, molluscs, crustaceans, and wheat in raw and processed foodstuffs. Within the scope of this document, minimum requirements for an LOQ (Limit of Quantification) will be derived from threshold data of allergic consumers. For quantitative antibody-based methods, a normative annex will describe what specific information the method developer needs to deliver and how performance characteristics shall be validated. Regarding PCR and LC-MS/MS, information on performance characteristics are in parts covered by EN 15634-1 and EN 17644. This document does not apply to fragmented or hydrolysed food allergens, such as casein hydrolysates or soy sauce. It also does not apply to methods that deliver qualitative results only. Methods that cover gluten-containing cereals (wheat, rye, and barley) with regard to coeliac disease are covered by EN 17254.

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This International standard method specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled HPLC-GC-FID. This standard is not intended to be applied to other matrices.
The method can be used for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH).
According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg.
In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS.
An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.5. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).

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This document specifies minimum performance requirements for methods that quantify the food allergens milk, egg, peanut, hazelnut, almond, brazil nut, macadamia nut, cashew, pistachio nut, walnut, pecan nut, lupine, sesame, mustard, soy, celery, fish, molluscs, crustaceans, and wheat in raw and processed foodstuffs. Within the scope of this document, minimum requirements for an LOQ (Limit of Quantification) will be derived from threshold data of allergic consumers. For quantitative antibody-based methods, a normative annex will describe what specific information the method developer needs to deliver and how performance characteristics shall be validated. Regarding PCR and LC-MS/MS, information on performance characteristics are in parts covered by EN 15634-1 and EN 17644. This document does not apply to fragmented or hydrolysed food allergens, such as casein hydrolysates or soy sauce. It also does not apply to methods that deliver qualitative results only. Methods that cover gluten-containing cereals (wheat, rye, and barley) with regard to coeliac disease are covered by EN 17254.

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This document specifies a method for the classification of grades of tea polyphenol extracts according to an analysis of their chemical characteristics. This document specifies requirements for tea polyphenol extracts in solid form.

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ISO 22935-1¦IDF 99-1 gives general guidance for the recruitment, selection, training, and monitoring of assessors for sensory analysis of milk and milk products.
It specifies criteria for the selection, and procedures for the training and monitoring, of selected assessors and expert sensory assessors for milk and milk products. It supplements the information given in ISO 8586-1 and parts of ISO 8586-2 that deal with expert sensory assessors.

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ISO 22935-2|IDF 99-2 specifies recommended methods for the sensory evaluation of specific milk and milk products. It specifies criteria for the sampling and preparation of samples and the assessment of the samples.
ISO 22935-2|IDF 99-2 is suitable for application in conjunction with the sensory methodologies outlined in ISO 22935-1|IDF 99-1 and other ISO or IDF sensory methodologies for specific situations and products.

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ISO 22935-3¦IDF 99-3 gives guidance on a general method for evaluation of compliance with product specifications for sensory properties based on sensory scoring and the use of a common nomenclature of terms.
The method is especially applicable in process and quality control performed regularly on a larger number of samples and/or with some time pressure and/or with a limited number of expert assessors available.
The results from the method may be part of product classification systems for domestic and international trade. Classification systems are not covered by ISO 22935-3¦IDF 99-3.

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  • Standard
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This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices.
The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH).
According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg.
In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS.
An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).

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The document specifies the definitions and technical criteria to be fulfilled for foods and food ingredients to be suitable for vegetarians (including ovo-lacto-, ovo- and lacto-vegetarians) or vegans as well as for food labelling and claims.
It is applicable to business-to-business communication (B2B), to the food trade, and to food labelling and claims. The definitions and technical criteria apply only post-harvest/collecting.
It does not apply to human safety, environmental safety, socio-economic considerations (e.g. fair trade, animal welfare), religious beliefs and the characteristics of packaging materials.

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This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices. The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH). According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg. In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS. An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).

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This document gives specifications, test methods, sampling, marking and labelling of porcelain tableware that is used for the preparation and serving of foods.

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This document specifies a reference method for the determination of lactose content of raw milk, heat-treated milks, dried milk, dairy permeate, dairy permeate powder, and raw and pasteurized cream. The method does not apply to fermented milks and milks to which oligosaccharides have been added, nor to low-lactose or lactose-free milks.

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This document specifies a method for the detection of linear condensed phosphates in meat and meat products by thin layer chromatographic separation. This document only applies to the detection of added condensed phosphates that are still present in the sample at the time of investigation, because condensed phosphates are gradually hydrolyzed by enzymes present in meat or meat products and during heat treatment of meat or meat products.

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This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of duck-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of duck material derived from mallard duck (Anas platyrhynchos) and spot-billed duck (Anas zonorhyncha). Cross detection of white-winged duck (Asarcornis scutulata), tufted duck (Aythya fuligula), muscovy duck (Cairina moschata) and Mandarin duck (Aix galericulata) is observed. Mallard duck and muscovy duck are domesticated poultry for food, while spot-billed duck, white-winged duck, tufted duck and Mandarin duck are wild avian. The target sequence is a partial fragment of the Anas platyrhynchos breed pekin duck isolate CAU_Pekin_2.0 Chr1, whole genome shotgun sequence (i.e. GenBank accession number JACEUM010000001.1),[1] which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).

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This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of pigeon-specific DNA derived from food and feed. The method can be applied to distinguish rock pigeon (Columba livia) from other domestic poultry meats (e. g. goose, duck, quail, pheasant). It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix. The target sequence is a partial fragment of Columba livia breed Danish Tumbler unplaced genomic scaffold, Cliv_1.0 scaffold114 (i.e. GenBank accession number NW_004973337.1),[1] which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).

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This document specifies a method for the determination of nitrite and nitrate in meat and meat products using ion chromatography.

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This document specifies a test method for the determination of aluminium in aqueous extracts.
It is applicable to paper and board with extractable aluminium content of at least 0,2 mg/l.
Aluminium extract levels below those given can be measured using this document if sensitive equipment
is available and if all other laboratory conditions fulfil the requirements for trace element analysis.

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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP)
(EC 3.1.3.1) activity in cheese.
This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the
surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific
conditions of sampling apply (see Clause 7).
The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits
per litre (mU/kg).

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  • Standard
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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC
3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks.
This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to
milk powder after reconstitution.
The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l).
If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not
higher than 7 000 mU/l.

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This European Standard specifies a method to determine the migration of substances from factory made cementitious products into test waters after contact with the products.
This European Standard is applicable to factory made cementitious products, e.g. cement mortar linings to metallic pipes, tanks, concrete pipes etc., intended to be used for the transport and storage of water intended for human consumption, including raw water used for the production of drinking water.

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The document specifies the definitions and technical criteria to be fulfilled for foods and food ingredients to be suitable for vegetarians (including ovo-lacto-, ovo- and lacto-vegetarians) or vegans as well as for food labelling and claims.
It is applicable to business-to-business communication (B2B), to the food trade, and to food labelling and claims. The definitions and technical criteria apply only post-harvest/collecting.
It does not apply to human safety, environmental safety, socio-economic considerations (e.g. fair trade, animal welfare), religious beliefs and the characteristics of packaging materials.

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This document specifies two methods for the quantitative determination of phthalates in vegetable oils by gas chromatography-mass spectrometry (GC-MS): — Part A for the determination of di-2-ethylhexyl phthalate (DEHP). — Part B for the determination of eight phthalates: dimethyl phthalate (DMP), diethyl phthalate (DEP), di-isobutyl phthalate (DIBP), dibutyl phthalate (DBP), benzylbutyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), di-isononyl phthalate (DINP), di-isodecyl phthalate (DIDP). Both methods are applicable for all vegetable oils, including crude, refined and virgin.

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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in cheese.
This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7).
The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits per litre (mU/kg).

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  • Standard
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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks.
This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l.

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  • Standard
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This document specifies the technical and functional characteristics for all types of thermometers (electronic, mechanical, etc.) for equipping the means used for the transport, storage and distribution of temperature sensitive goods and for measuring the ambient or internal temperature of the products between -80 °C and +85 °C.
It specifies the test methods which allow the verification of the equipment's conformity to suitability and performance requirements.
It applies to the whole indicator-temperature sensor(s). The temperature sensor(s) can be integrated into the thermometer or remote from it (external temperature sensor(s)).
It does not define the location of the thermometer and its sensors with respect to types of usage such as transport, storage and distribution.
NOTE   Examples for the transport, storage and distribution of temperature sensitive goods between -80 °C and +85 °C include chilled, frozen, deep frozen and quick frozen food; ice cream; fresh and hot food; pharmaceuticals; blood and organs; chemicals; biologicals; electronic and mechanical devices; flowers, plants and bulbs; raw materials and liquids; animals; art and furnishings.

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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution. The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l.

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  • Standard
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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in cheese. This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7). The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits per litre (mU/kg).

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This document specifies the verification procedure for temperature recorders and thermometers for measuring the air and the products between −80 °C and +85 °C, which are intended to equip the means used for the transport, storage and distribution of temperature sensitive goods and which comply with standards EN 12830 and EN 13485 (measurement classes and ranges).
It specifies the test methods which allow the verification of the equipment's conformity against class requirements identified in EN 12830 and EN 13485.
NOTE Examples for the transport, storage and distribution of temperature sensitive goods between −80 °C and +85 °C include chilled, frozen, deep frozen and quick-frozen food; ice cream; fresh and hot food; pharmaceuticals; blood and organs; chemicals; biologicals; electronic and mechanical devices; flowers, plants and bulbs; raw materials and liquids; animals; art and furnishings.

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This European Standard specifies a method to determine the migration of substances from factory made cementitious products into test waters after contact with the products.
This European Standard is applicable to factory made cementitious products, e.g. cement mortar linings to metallic pipes, tanks, concrete pipes etc., intended to be used for the transport and storage of water intended for human consumption, including raw water used for the production of drinking water.

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This document specifies the technical and functional characteristics for all types of thermometers (electronic, mechanical, etc.) for equipping the means used for the transport, storage and distribution of temperature sensitive goods and for measuring the ambient or internal temperature of the products between −80 °C and +85 °C.
It specifies the test methods which allow the verification of the equipment's conformity to suitability and performance requirements.
It applies to the whole thermometer and indicating device(s). The temperature sensor(s) can be integrated into the thermometer or remote from it (wired or wireless external temperature sensor(s)).
It does not specify the location of the thermometer and its sensors with respect to types of usage such as transport, storage and distribution.
NOTE   Examples for the transport, storage and distribution of temperature sensitive goods between −80 °C and +85 °C include chilled, frozen, deep frozen and quick-frozen food; ice cream; fresh and hot food; pharmaceuticals; blood and organs; chemicals; biologicals; electronic and mechanical devices; flowers, plants and bulbs; raw materials and liquids; animals; art and furnishings.

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This document specifies a test method for the determination of aluminium in aqueous extracts.
It is applicable to paper and board with extractable aluminium content of at least 0,2 mg/l.
Aluminium extract levels below those given can be measured using this document if sensitive equipment is available and if all other laboratory conditions fulfil the requirements for trace element analysis.

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This document specifies the verification procedure for temperature recorders and thermometers for measuring the ambient or internal temperature of the products between −80 °C and +85 °C, which are intended to equip the means used for the transport, storage and distribution of temperature sensitive goods and which comply with standards EN 12830 and EN 13485 (measurement classes and ranges) and also others used for transport, distribution and/or storage of temperature sensitive goods.
It specifies the test methods which allow the verification of the equipment's conformity against class requirements identified in EN 12830 and EN 13485.
NOTE   Examples for the transport, storage and distribution of temperature sensitive goods between −80 °C and +85 °C include chilled, frozen, deep frozen and quick-frozen food; ice cream; fresh and hot food; pharmaceuticals; blood and organs; chemicals; biologicals; electronic and mechanical devices; flowers, plants and bulbs; raw materials and liquids; animals; art and furnishings.

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This European Standard specifies a method to determine the influence of factory made cementitious products on the odour, flavour, colour and turbidity of test waters after contact with the products.
This European Standard is applicable to factory made cementitious products, e.g. cement mortar linings to metallic pipes, tanks, concrete pipes etc. intended to be used for the transport and storage of water for human consumption, including raw water used for the production of drinking water.

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This European Standard specifies a method to determine the influence of factory made cementitious products on the odour, flavour, colour and turbidity of test waters after contact with the products.
This European Standard is applicable to factory made cementitious products, e.g. cement mortar linings to metallic pipes, tanks, concrete pipes etc. intended to be used for the transport and storage of water for human consumption, including raw water used for the production of drinking water.

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This document specifies the characteristics, sampling, packaging and marking of concentrated mulberry juice. This document is applicable to concentrated black mulberry juice that is physically processed and packaged for human consumption.

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This document specifies a method for the determination of the saponification value of animal and vegetable fats and oils. The saponification value is a measure of the free and esterified acids present in fats and fatty acids.
The method is applicable to refined and crude vegetable and animal fats.
If mineral acids are present, the results given by this method are not interpretable unless the mineral acids are determined separately.
The saponification value can also be calculated from fatty acid data obtained by gas chromatography analysis as given in Annex B. For this calculation, it is necessary to be sure that the sample does not contain major impurities or is thermally degraded.

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This deliverable specifies a method for the determination of the moisture content of all types of dried milk. The revised version will include the results of a recently conducted interlaboratory study in whey powders, dairy permeate powders, cream powder and powdered infant formula in ISO 5537|IDF 26 to further underpin the extent of its scope.

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This document specifies a procedure for the determination of Ag, As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Se, Tl,
U and Zn in foodstuffs by inductively coupled plasma mass spectrometry (ICP-MS) after pressure
digestion.
The following foodstuffs were analysed for the elements listed in Table 1 in an interlaboratory study:
Banana (deep-frozen), Cocoa powder, Wheat noodle powder, Currant nectar (deep-frozen), Milk powder,
Oyster (dried), Celery (dried), Dogfish liver (dried), Liver (deep-frozen), Kale (dried).

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This document specifies a reference method for the determination of the nitrogen content of meat and meat products by the Kjeldahl principle.

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This document specifies two reference methods for the determination of the moisture content of meat and meat products: a direct drying method and a distillation method. The direct drying method is applicable to meat and meat products with low volatile substances in addition to moisture. The distillation method is applicable to meat and meat products with high volatile substances in addition to moisture.

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This document specifies a procedure to evaluate the passive behaviour of stainless steels and other passive alloys used in construction products intended to come into contact with drinking water.
The passive state is the reason why no relevant amounts of metals are released from such materials into the drinking water. This test is used to verify whether the alloy under consideration is passive under conditions which can occur in drinking waters.
This document is not applicable for product testing. It is only applicable for the assessment of passive behaviour of materials.

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