High fructose syrup — Specifications and test methods

This document specifies the chemical and microbiological requirements for and test methods of high fructose syrup.

Sirop à haute teneur en fructose — Spécifications et méthodes d'essai

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ISO 24683:2022 - High fructose syrup — Specifications and test methods Released:5. 08. 2022
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First edition
High fructose syrup — Specifications
and test methods
Sirop à haute teneur en fructose — Spécifications et méthodes d'essai
Reference number
ISO 24683:2022(E)
© ISO 2022

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ISO 24683:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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  © ISO 2022 – All rights reserved

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ISO 24683:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 2
4.1 Physical indices . 2
4.2 Chemical indices. 2
4.3 Contaminant limits . 3
4.4 Microbial limits . 3
5 Test methods . 3
5.1 pH . 3
5.2 Sulfur dioxide . . 3
5.3 Fructose and glucose content . 3
5.3.1 General . 3
5.3.2 Equipment . 3
5.3.3 Reagents . 4
5.3.4 Instrument parameters . 4
5.3.5 Procedure . 4
5.4 Hydroxymethylfurfural (HMF) . 4
5.4.1 Eluent preparation . 4
5.4.2 Sample preparation . 4
5.4.3 Standard preparation. 5
5.4.4 HPLC parameters . 5
5.4.5 Calculation . 5
5.5 Electrical conductivity . 5
5.5.1 Equipment . 5
5.5.2 Reagents . 5
5.5.3 Standardization procedure . 5
5.5.4 Analysis . 5
5.6 Chloride . 6
5.6.1 General . 6
5.6.2 Reagents . 6
5.6.3 Procedure . 6
5.6.4 Calculation . 6
5.7 Copper . 6
6 Marking, packaging, transportation and storage requirements.7
6.1 Marking . 7
6.2 Packaging . 7
6.3 Transport . 7
6.4 Storage . 7
Bibliography . 8
© ISO 2022 – All rights reserved

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ISO 24683:2022(E)
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
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any patent rights identified during the development of the document will be in the Introduction and/or
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This document was prepared by Technical Committee ISO/TC 93, Starch (including derivatives and by-
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
  © ISO 2022 – All rights reserved

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ISO 24683:2022(E)
High fructose syrup (HFS) is a sweetener made from starch. The starch is first broken down into
glucose by enzymes and is then further processed by glucose isomerase to convert some of its glucose
into fructose. “HFS 42” refers to 42 % and “HFS 55” to 55 % fructose composition, respectively. HFS 42
is mainly used for processed foods and breakfast cereals, whereas HFS 55 is used mostly for the
production of soft drinks.
© ISO 2022 – All rights reserved

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High fructose syrup — Specifications and test methods
1 Scope
This document specifies the chemical and microbiological requirements for and test methods of high
fructose syrup.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 1743, Glucose syrup — Determination of dry matter content

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