ISO 16179:2025
(Main)Footwear — Critical substances potentially present in footwear and footwear components — Determination of organotin compounds in footwear materials
Footwear — Critical substances potentially present in footwear and footwear components — Determination of organotin compounds in footwear materials
This document specifies a test method for the qualification and quantification of organotin compounds by applying gas chromatography coupled with mass spectrometry. This test method is applicable to all types of footwear materials except metal hardware (see ISO/TR 16178).
Chaussures — Substances critiques potentiellement présentes dans les chaussures et les composants de chaussures — Détermination des composés organostanniques dans les matériaux de chaussures
L'ISO/TS 16179:2012 spécifie une méthode d'essai permettant de déterminer la présence de composés organostanniques dans les matériaux de chaussures. Cette méthode s'applique à tous les types de matériaux de chaussures.
General Information
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Standards Content (Sample)
International
Standard
ISO 16179
Second edition
Footwear — Critical substances
2025-03
potentially present in footwear
and footwear components —
Determination of organotin
compounds in footwear materials
Chaussures — Substances critiques potentiellement présentes
dans les chaussures et les composants de chaussures —
Détermination des composés organostanniques dans les
matériaux de chaussures
Reference number
© ISO 2025
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ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus and materials . 3
7 Preparation of the sample . 3
8 Procedure . 4
8.1 General .4
8.2 Preparation of the sodium tetraethylborate solution .4
8.3 Preparation of standard solutions .4
8.3.1 General .4
8.3.2 Internal standards — stock solution (1 000 mg/l of organotin cation) .5
8.3.3 Internal standards — working solution (10 mg/l of organotin cation) .6
8.3.4 Target compounds — stock solution (1 000 mg/l of organotin cation) .6
8.3.5 Target compounds — working solution (10 mg/l of organotin cation) .6
8.4 Preparation of the tropolone solution .6
8.5 Preparation of the buffer solution .6
8.6 Preparation of the calibration solutions .6
8.7 Sample preparation .7
8.8 Preparation of the blank solution .7
8.9 Gas chromatography .7
8.9.1 General .7
8.9.2 With MS .7
8.9.3 With MS/MS .8
9 Expression of the results . 8
9.1 Determination of the mass fraction of the tin organic compounds .8
9.2 Precision of the test method .9
10 Test report . 9
Annex A (informative) Gas chromatography apparatus and conditions for organotin analysis .10
Annex B (informative) Suitable conditions for mass spectroscopy .11
Annex C (informative) Reliability of the method .13
Bibliography . 14
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
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with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
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Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 216, Footwear, in collaboration with the
European Committee for Standardization (CEN) Technical Committee CEN/TC 309, Footwear, in accordance
with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
This second edition cancels and replaces the first edition (ISO/TS 16179:2012), which has been technically
revised.
The changes are as follows:
— the technical specification becomes an ISO standard;
— GC-MS/MS technique added in 8.9.3 and in Clause B.2;
— new extraction solvent in Clause 4;
— new Table 1 (certain substances added) in Clause 4;
— change in the safety instructions at the preparation of the sodium tetraethylborate solution in Clause 8;
— sample preparation reference to ISO 21061 in Clause 7;
— new Table 2 (certain substances added) in 8.3.1;
— deletion of the need for duplicate determinations in Clause 7;
— new Annex B for mass spectroscopy;
— deletion of the reference to ISO 22744-1 in the Bibliography.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
Certain organotin compounds have been identified as carcinogenic. Thus, several countries have restricted
[1]
them in articles such as footwear, e.g. in the European Union by commission regulation (EU) 276/2010
[2]
amending regulation (EC) No 1907/2006 .
Further organotin compounds are restricted by footwear brands in their restricted substances lists (RSL).
v
International Standard ISO 16179:2025(en)
Footwear — Critical substances potentially present in
footwear and footwear components — Determination of
organotin compounds in footwear materials
1 Scope
This document specifies a test method for the qualification and quantification of organotin compounds by
applying gas chromatography coupled with mass spectrometry. This test method is applicable to all types of
footwear materials except metal hardware (see ISO/TR 16178).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 4787, Laboratory glass and plastic ware — Volumetric instruments — Methods for testing of capacity and for use
ISO 21061, Footwear — Chemical tests — General principles on the preparation of samples
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
4 Principle
The organotin substances are extracted from the footwear material with methanol, in a medium-strength
acidic condition, using tropolone as a complexing agent.
In the previous version of this test method, the extraction solvent was a mixture of methanol-ethanol.
Comparisons have shown that methanol gives equivalent results to methanol-ethanol solution. It is still
possible to use extraction with methanol-ethanol solution.
The polar and high-boiling organotin is then converted to the corresponding volatile alkyl derivative, by
reaction with sodium tetraethylborate, NaB(Et) . Finally, it is detected and quantified by using a gas
chromatograph fitted with a mass selective detector [gas chromatograph with a single quadrupole mass
spectrometer (GC-MS) or gas chromatograph with a triple quadrupole mass spectrometer (GC-MS/MS)].
Table 1 indicates the list of target compounds which can be analysed with the method defined in this
document. This document is also applicable for further organotin substances provided that the method is
validated with the additional compounds.
Table 1 — List of target compounds and internal standards that can be analysed
® b
Type of compound Compound CAS Registry Number
Monosubstituted Internal standard: n-h
...
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