Synthetic rubber latex — Examination for microorganisms

ISO 20851:2017 specifies the method to examine the presence and approximate count of viable aerobic and facultative anaerobic microorganisms in synthetic rubber latices. Identification of the microorganisms is outside the scope of this document.

Latex de caoutchouc synthétique — Examen des micro-organismes

General Information

Status
Published
Publication Date
07-Aug-2017
Current Stage
9093 - International Standard confirmed
Completion Date
01-Jul-2022
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ISO 20851:2017 - Synthetic rubber latex -- Examination for microorganisms
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INTERNATIONAL ISO
STANDARD 20851
First edition
2017-07
Synthetic rubber latex — Examination
for microorganisms
Latex de caoutchouc synthétique — Examen des micro-organismes
Reference number
ISO 20851:2017(E)
ISO 2017
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ISO 20851:2017(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2017, Published in Switzerland

All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form

or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior

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ii © ISO 2017 – All rights reserved
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ISO 20851:2017(E)
Contents Page

Foreword ........................................................................................................................................................................................................................................iv

Introduction ..................................................................................................................................................................................................................................v

1 Scope ................................................................................................................................................................................................................................. 1

2 Normative references ...................................................................................................................................................................................... 1

3 Terms and definitions ..................................................................................................................................................................................... 1

4 Principle ........................................................................................................................................................................................................................ 1

5 Reagent ........................................................................................................................................................................................................................... 2

6 Apparatus ..................................................................................................................................................................................................................... 2

7 Sampling ........................................................................................................................................................................................................................ 2

8 Procedure..................................................................................................................................................................................................................... 2

9 Examination .............................................................................................................................................................................................................. 3

9.1 Examination of the microbial count (CFU/ml) ............................................................................................................ 3

9.2 Interpretation .......................................................................................................................................................................................... 3

10 Disposal of the used slides and equipment .............................................................................................................................. 3

11 Test report ................................................................................................................................................................................................................... 3

Annex A (informative) Example of a model chart ................................................................................................................................... 4

Annex B (informative) Interpretation of the microbial count ................................................................................................... 5

© ISO 2017 – All rights reserved iii
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ISO 20851:2017(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards

bodies (ISO member bodies). The work of preparing International Standards is normally carried out

through ISO technical committees. Each member body interested in a subject for which a technical

committee has been established has the right to be represented on that committee. International

organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.

ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of

electrotechnical standardization.

The procedures used to develop this document and those intended for its further maintenance are

described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the

different types of ISO documents should be noted. This document was drafted in accordance with the

editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of

any patent rights identified during the development of the document will be in the Introduction and/or

on the ISO list of patent declarations received (see www .iso .org/ patents).

Any trade name used in this document is information given for the convenience of users and does not

constitute an endorsement.

For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and

expressions related to conformity assessment, as well as information about ISO’s adherence to the

World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following

URL: w w w . i s o .org/ iso/ foreword .html

This document was prepared by Technical Committee ISO/TC 45, Rubber and rubber products,

Subcommittee SC 3, Raw materials (including latex) for use in the rubber industry.

iv © ISO 2017 – All rights reserved
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ISO 20851:2017(E)
Introduction

Synthetic latices are susceptible to post-production contamination with microorganisms during storage

and shipment. Unless precautions are taken, such as maintenance of a high pH, the addition of biocide

and inspection and cleaning of tanks, these organisms may proliferate, ultimately producing unpleasant

odours and changes in the chemical and physical properties of the latex. It is highly desirable to be able

to detect the presence of significant microorganisms before such changes develop.

This document replaces ISO 9252:1989, Synthetic rubber latex – Microbiological examination, which

was withdrawn due to its obsolescence and complicated testing procedures. This document provides

a far more simplified method for the purpose of microbiological examination, employing a ready-made

medium slide as reagent.
© ISO 2017 – All rights reserved v
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INTERNATIONAL STANDARD ISO 20851:2017(E)
Synthetic rubber latex — Examination for microorganisms

WARNING 1 — Persons using this document should be familiar with normal laboratory practice.

This document does not purport to address all of the safety problems, if any, associated with its

use. It is the responsibility of the user to establish appropriate safety and health practices and to

ensure compliance with any national regulatory conditions.
WARNING 2 — Certain procedures
...

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