ISO/TS 24420:2023

Biotechnology — Massively parallel DNA sequencing — General requirements for data processing of shotgun metagenomic sequences

This document illustrates the workflow of shotgun metagenomic sequence data processing of host-derived microbiome and environmental metagenomes. This document specifies the requirements for quality control of shotgun metagenomic sequence data processing for massively parallel DNA sequencing. This document provides guidelines for data directory, data archive and metadata for shotgun metagenomic sequence data. This document applies to data storage, sharing and interoperability of shotgun metagenomic sequence data. This document applies to shotgun metagenomic sequence data processing and analyses, but excludes functional analysis.

Biotechnologie — Séquençage d'ADN massivement parallèle — Exigences générales pour le traitement des données des séquences métagénomiques "Shotgun"

General Information

• Status: Published
• Publication Date: 18-May-2023
• ICS: 07.080 - Biology. Botany. Zoology
• Technical Committee: ISO/TC 276 - Biotechnology
• Drafting Committee: ISO/TC 276/WG 5 - Data processing and integration
• Current Stage: 6060 - International Standard published
• Start Date: 19-May-2023
• Due Date: 22-Jun-2023
• Completion Date: 19-May-2023

Standards Content (Sample)

TECHNICAL ISO/TS
SPECIFICATION 24420
First edition
2023-05
Biotechnology — Massively parallel
DNA sequencing — General
requirements for data processing of
shotgun metagenomic sequences
Biotechnologie — Séquençage d'ADN massivement parallèle —
Exigences générales pour le traitement des données des séquences
métagénomiques "Shotgun"
Reference number
ISO/TS 24420:2023(E)
© ISO 2023

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ISO/TS 24420:2023(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
  © ISO 2023 – All rights reserved

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ISO/TS 24420:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Processing workflow .4
5 Data processing . 5
5.1 Facilities and software requirements . 5
5.2 Sequence quality control and error determination . 5
5.3 Sequence assembly . 6
6 Data analysis . 6
6.1 Annotation . . 6
6.2 Calculation of species relative abundance . 7
6.2.1 Species analysis . 7
6.2.2 Gene analysis . 7
7 Data archive and metadata . 7
7.1 Original data . 7
7.2 Sequencing analytical data . 7
7.3 Data directory and archive .
...

FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 24420
ISO/TC 276
Biotechnology — Massively parallel
Secretariat: DIN
DNA sequencing — General
Voting begins on:
2023-02-20 requirements for data processing of
shotgun metagenomic sequences
Voting terminates on:
2023-04-17
Biotechnologie — Séquençage d'ADN massivement parallèle —
Exigences générales pour le traitement des données des séquences
métagénomiques "Shotgun"
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 24420:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 1 ----------------------
ISO/DTS 24420:2023(E)
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 24420
ISO/TC 276
Biotechnology — Massively parallel
Secretariat: DIN
DNA sequencing — General
Voting begins on:
requirements for data processing of
shotgun metagenomic sequences
Voting terminates on:
Biotechnologie — Séquençage d'ADN massivement parallèle —
Exigences générales pour le traitement des données des séquences
métagénomiques "Shotgun"
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/DTS 24420:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN­
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

---------------------- Page: 2 ----------------------
ISO/DTS 24420:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Processing workflow .4
5 Data processing . 5
5.1 Facilities and software requirements . 5
5.2 Sequence quality control and error determination . 5
5.3 Sequence assembly . 6
6 Data analysis .
...

© ISO 2022 – All rights reserved
ISO/DTS.2 24420:20222023(E)
Date: 2023-01-09
ISO/TC 276
Secretariat: DIN
Biotechnology — Massively parallel DNA sequencing — General requirements for data
processing of shotgun metagenomic sequences
Biotechnologie — Séquençage d'ADN massivement parallèle -— Exigences générales pour le
traitement des données des séquences métagénomiques "Shotgun"

DTS.2 stage

Warning for WDs and CDs
This document is not an ISO International Standard. It is distributed for review and comment.
It is subject to change without notice and may not be referred to as an International Standard.
Recipients of this draft are invited to submit, with their comments, notification of any relevant
patent rights of which they are aware and to provide supporting documentation.
To help you, this guide on writing standards was produced by the ISO/TMB and is available at
http://www.iso.org/iso/how-to-write-standards.pdf
A model manuscript of a draft International Standard (known as “The Rice Model”) is available at
http://www.iso.org/iso/model_document-rice_model.pdf

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© ISO 2022

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ISO/DTS.2 24420:20222023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part
of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or
mechanical, including photocopying, or posting on the internet or an intranet, without prior written
permission. Permission can be requested from either ISO at the address below or ISO’s member body
in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.orgwww.iso.org
Published in Switzerland
Formatted: Font: 11 pt
Formatted: Space Before: 0 pt, Line spacing: Exactly 11
pt, Tab stops: Not at 487.6 pt
2 © ISO 2022 – All rights reserved
ii © ISO 2023 – All rights reserved

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ISO/DTS.2 24420:20222023(E)
Contents
Foreword . 5
Introduction. 6
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Processing workflow . 5
5 Data processing . 6
5.1 Facilities and software requirements . 6
5.2 Sequence quality control and error determination . 6
5.3 Sequence assembly . 7
6 Data analysis . 7
6.1 Annotation . 7
6.2 Calculation of species relative abundance . 8
7 Data archive and metadata . 8
7.1 Original data . 8
7.2 Sequencing analytical data . 9
7.3 Data directory and archive . 9
Annex A .
...