ISO 20304-2:2024
(Main)Fine bubble technology — Water treatment applications — Part 2: Test methods using Escherichia coli as a test micro-organism
Fine bubble technology — Water treatment applications — Part 2: Test methods using Escherichia coli as a test micro-organism
This document specifies a test method for assessing bactericidal viability of Escherichia coli as a test micro-organism, in dispersions of various fine bubbles generated by the hydrodynamic cavitation of water medium.
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FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 20304-2
ISO/TC 281
Fine bubble technology — Water
Secretariat: JISC
treatment applications —
Voting begins on:
2023-09-05
Part 2:
Voting terminates on:
Test methods using Escherichia coli as
2023-10-31
a test micro-organism
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 20304-2:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 1 ----------------------
ISO/FDIS 20304-2:2023(E)
FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 20304-2
ISO/TC 281
Fine bubble technology — Water
Secretariat: JISC
treatment applications —
Voting begins on:
Part 2:
Voting terminates on:
Test methods using Escherichia coli as
a test micro-organism
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
CH-1214 Vernier, Geneva
DOCUMENTATION.
Phone: +41 22 749 01 11
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: copyright@iso.org
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 20304-2:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
© ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 2 ----------------------
ISO/FDIS 20304-2:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Safety precautions. 2
5 Requirements . 2
6 Test methods . 2
6.1 Principle . 2
6.2 Test facility . 2
6.3 Culture media and reagents. 2
6.3.1 General .
...
ISO/DISFDIS 20304-2:2023(E)
2023-05-10
ISO/TC 281/WG 3
Secretariat: JISC
Date: 2023-07-25
Fine bubble technology — Water treatment applications —
Part 2:
Test methods using Escherichia coli as a test micro-organism
FDIS stage
© ISO 2023 – All rights reserved
---------------------- Page: 1 ----------------------
ISO/DISFDIS 20304-2:2023(E)
© ISO 20222023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this
publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical,
including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can
be requested from either ISO at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
EmailE-mail: copyright@iso.org
Website: www.iso.orgwww.iso.org
Published in Switzerland
ii © ISO2022 © ISO 2023 – All rights reserved
---------------------- Page: 2 ----------------------
ISO/DISFDIS 20304-2:2023(E)
Contents
Foreword . v
Introduction . vi
Part 2: Test methods using Escherichia coli as a test micro-organism . 1
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Safety precautions . 2
5 Requirements . 2
6 Test methods . 2
6.1 Principle . 2
6.2 Test facility . 2
6.3 Culture media and reagents . 2
6.3.1 General . 2
6.3.2 Water . 3
6.3.3 Tryptone soya agar (TSA) . 3
6.3.4 Tryptone soya broth (TSB) . 3
6.3.5 Lysogeny broth (LB) . 3
6.3.6 Cryoprotective solution for bacterial species . 3
6.3.7 Physiological saline . 4
6.3.8 Tryptone sodium chloride solution (TSCS) . 4
6.4 Laboratory apparatus and glassware . 4
6.5 Preparation of bacterial test suspension .
...
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