ISO 4730
(Main)Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
ISO 4730:2017 specifies certain characteristics of the essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil), in order to facilitate assessment of its quality.
Huile essentielle de Melaleuca, type terpinén-4-ol (huile essentielle de Tea Tree)
ISO 4730:2017 spécifie certaines caractéristiques de l'huile essentielle de Melaleuca, type terpinèn-4-ol (huile essentielle de «Tea Tree»), destinées à faciliter l'évaluation de sa qualité.
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International
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ISO/FDIS 4730
ISO/TC 54
Essential oil of Melaleuca, terpinen-
Secretariat: UNE
4-ol type (tea tree oil)
Voting begins on:
2025-03-31
Voting terminates on:
2025-05-26
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ISO/FDIS 4730:2025(en) © ISO 2025
FINAL DRAFT
ISO/FDIS 4730:2025(en)
International
Standard
ISO/FDIS 4730
ISO/TC 54
Essential oil of Melaleuca, terpinen-
Secretariat: UNE
4-ol type (tea tree oil)
Voting begins on:
Voting terminates on:
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2025
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
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CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
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Published in Switzerland Reference number
ISO/FDIS 4730:2025(en) © ISO 2025
ii
ISO/FDIS 4730:2025(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 1
4.1 General .1
4.2 Chromatographic profile .2
5 Additional information . 2
5.1 Flashpoint .2
5.2 Enantiomeric distribution .3
6 Sampling . 3
7 Packaging, labelling, marking and storage . 3
Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the
essential oil of Melaleuca, terpinen-4-ol type (tea tree oil) . 4
Annex B (informative) Flashpoint . 7
Annex C (informative) Enantiomeric distribution — General information . 8
Bibliography . 9
iii
ISO/FDIS 4730:2025(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 54, Essential oils.
This fourth edition cancels and replaces the third edition (ISO 4730:2017), which has been technically
revised. It also incorporates the Amendment ISO 4730:2017/Amd 1:2018.
The main changes compared to the previous edition are as follows:
— inclusion of enantiomeric distribution parameters for α-terpineol.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
FINAL DRAFT International Standard ISO/FDIS 4730:2025(en)
Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
1 Scope
This document specifies certain characteristics of the essential oil of Melaleuca, terpinen-4-ol type (tea tree
oil), in order to facilitate assessment of its quality.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 210, Essential oils — General requirements and guidelines for packaging, conditioning and storage
ISO 211, Essential oils — General requirements for labelling and marking of containers
ISO 212, Essential oils — Sampling
ISO 279, Essential oils — Determination of relative density at 20 °C — Reference method
ISO 280, Essential oils — Determination of refractive index
ISO 592, Essential oils — Determination of optical rotation
ISO 875, Essential oils — Evaluation of miscibility in ethanol
ISO 11024 (all parts), Essential oils — General guidance on chromatographic profiles
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology
...
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ISO/FDIS 4730:2025(E) .
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ISO /TC 54
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Secretariat: UNE
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Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
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ISO/FDIS 4730:2025(Een)
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at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
Fax: +41 22 749 09 47
EmailE-mail: copyright@iso.org
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© ISO 2025 – All rights reserved
iii
ISO/FDIS 4730:2025(Een)
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Formatted: HeaderCentered, Line spacing: single
Formatted: Font: Bold
Contents
Foreword . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements. 1
5 Additional information . 3
6 Sampling . 3
7 Packaging, labelling, marking and storage . 3
Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the
essential oil of Melaleuca, terpinen-4-ol type (tea tree oil) . 4
Annex B (informative) Flashpoint . 9
Annex C (informative) Enantiomeric distribution — General information . 10
Bibliography . 11
Foreword . iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements. 2
4.1 General . 2
4.2 Chromatographic profile . 2
5 Additional information . 3
5.1 Flashpoint . 3
5.2 Enantiomeric distribution . 3
6 Sampling . 3
7 Packaging, labelling, marking and storage . 3
Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the
essential oil of Melaleuca, terpinen-4-ol type (tea tree oil) . 4
Annex B (informative) Flashpoint . 7
Annex C (informative) Enantiomeric distribution — General information . 8
Bibliography . 9
Formatted: FooterPageRomanNumber, Line spacing:
single
Formatted: Font: 11 pt
iv © ISO 2025 – All rights reserved
iv
ISO/FDIS 4730:2025(Een)
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Formatted: HeaderCentered, Left, Line spacing: single
Formatted: Font: Bold
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types of
ISO documentsdocument should be noted. This document was drafted in accordance with the editorial rules
of the ISO/IEC Directives, Part 2 (see www.iso.org/directives 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent rights
in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of (a)
Formatted: Font: Not Italic, Font color: Auto
patent(s) which may be required to implement this document. However, implementers are cautioned that this
may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents.www.iso.org/patents. ISO shall not be held responsible for identifying any or all such
patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
Formatted: Font color: Auto
Field Code Changed
This document was prepared by Technical Committee ISO/TC 54, Essential oils.
This fourth edition cancels and replaces the third edition (ISO 4730:2017), which has been technically revised.
It also incorporates the Amendment ISO 4730:2017/Amd 1:2018.
The main changes compared to the previous edition are as follows:
— — Inclusioninclusion of enantiomeric distribution parameters for α-terpineol.
Formatted: List Continue 1, Indent: Left: 0 cm, First
line: 0 cm
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
Formatted: English (United Kingdom)
Field Code Changed
Formatted: Font: 11 pt
Formatted: FooterPageRomanNumber, Line spacing:
single
© ISO 2025 – All rights reserved
v
ISO/FDIS 4730:2025(en)
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Formatted: Main Title 1, Space After: 0 pt
Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
Formatted: Bold Italic, Font: Not Italic
1 Scope
Formatted: Font: Bold
This document specifies certain characteristics of the essential oil of Melaleuca, terpinen-4-ol type (tea tree
oil), in order to facilitate assessment of its quality.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 210, Essential oils — General requirements and guidelines for packaging, conditioning and storage
ISO 211, Essential oils — General requirements for labelling and marking of containers
ISO 212, Essential oils — Sampling
ISO 279, Essential oils — Determination of relative density at 20 °C — Reference method
ISO 280, Essential oils — Determination of refractive index
ISO 592, Essential oils — Determination of optical rotation
ISO 875, Essential oils — Evaluation of miscibility in ethanol
ISO 11024 (all parts), Essential oils — General guidance on chromatographic profiles
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminologicalterminology databases for use in standardization at the following
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addresses:
— — ISO Online browsing platform: available at https://www.iso.org/obphttps://www.iso.org/obp
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line: 0 cm
— — IEC Electropedia: available at http://www.electropedia.org/https://www.electropedia.org/
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line: 0 cm, Don't keep with next
3.1 3.1
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essential oil of Melaleuca, terpinen-4-ol type
tea tree oil
essential oil obtained by steam distillation of the leaves and terminal branchlets of Melaleuca alternifolia
(Maiden et Betche) Cheel or of M. linariifolia Sm
Note 1 to entry: For information on the characterization of this essential oil, see ISO/TR 21092.
Formatted: Note
4 Requirements
4.1 General
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Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil) shall meet the requirements according toand follow
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the test methods given in Table 1.Table 1.
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© ISO 2025 – All rights reserved
ISO/FDIS 4730:2025(en)
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Table 1 — Requirements for the essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
Characteristics Requirements ISO test method
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Appearance Clear, mobile liquid —
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Colour Colourless to pale yellow — Formatted: Font: Not Bold
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Odour Characteristic —
pt
Relative density at 20 °C, d
0,885 to 0,906 ISO 279 Formatted Table
𝑑
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Refractive index at 20 °C 1,475 to 1,482 ISO 280
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Optical rotation Between +7° and +12° ISO 592
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Miscibility in ethanol 85 % No more than 2 volumes of ethanol 85 % ISO 875
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(volume fraction) at 20 °C
...
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