SIST EN ISO 10705-3:2025
(Main)Water quality - Detection and enumeration of bacteriophages - Part 3: Validation of methods for concentration of bacteriophages from water (ISO 10705-3:2003)
Water quality - Detection and enumeration of bacteriophages - Part 3: Validation of methods for concentration of bacteriophages from water (ISO 10705-3:2003)
This part of ISO 10705 specifies the general principles for assessing the performance of methods for the concentration of bacteriophages from water. Concentration is recommended for those water samples expected to contain < 3 pfp (plaque-forming particles) per millilitre. Concentration methods can be applied to all kinds of water provided that the amount and nature of suspended solids and/or dissolved matter do not interfere with the concentration procedure.
This part of ISO 10705 does not give specific details of concentration methods, but outlines the fundamental
principles for evaluating the suitability of a particular method for a given type and volume of water. Annex A
gives examples of methods that have been found satisfactory and their fields of application.
Wasserbeschaffenheit - Nachweis und Zählung von Bakteriophagen - Teil 3: Validierung von Verfahren für die Konzentration von Bakteriophagen in Wasser (ISO 10705-3:2003)
Dieser Teil von ISO 10705 legt die allgemeinen Grundsätze für die Bewertung der Leistungsfähigkeit von Verfahren für die Aufkonzentrierung von Bakteriophagen aus Wasser fest. Eine Aufkonzentrierung wird für Wasserproben empfohlen, die voraussichtlich < 3 pfp (Plaque formende Partikel) je Milliliter enthalten. Aufkonzentrierungsverfahren können bei allen Arten von Wasser angewendet werden, sofern die Menge und Beschaffenheit der suspendierten und/oder gelösten Stoffe das Aufkonzentrierungsverfahren nicht beeinträchtigen.
Dieser Teil von ISO 10705 enthält keine detaillierten Angaben zu Aufkonzentrierungsverfahren, sondern umreißt die grundlegenden Prinzipien zur Bewertung der Eignung eines bestimmten Verfahrens für eine bestimmte Art und Menge von Wasser. Anhang A enthält Beispiele für Verfahren, die sich als zufriedenstellend erwiesen haben, und deren Anwendungsbereiche.
WARNHINWEIS — Anwender dieses Teils von ISO 10705 sollten mit der üblichen Laborpraxis vertraut sein. In diesem Teil von ISO 10705 wird nicht der Anspruch erhoben, alle bei seiner Anwendung möglicherweise auftretenden Sicherheitsprobleme zu behandeln. Es liegt in der Verantwortung des Arbeitsgebers, geeignete Sicherheits- und Gesundheitspraktiken vorzusehen und die Einhaltung etwaiger nationaler Bestimmungen sicherzustellen.
WICHTIG — Das mit der Validierung von Verfahren für die Aufkonzentrierung von Bakteriophagen aus Wasser befasste Laborpersonal muss unbedingt über einschlägige Erfahrungen mit den Verfahren zur Zählung von Bakteriophagen verfügen (siehe ISO/TR 13843 [1]).
Qualité de l'eau - Détection et dénombrement des bactériophages - Partie 3: Validation des méthodes de concentration des bactériophages dans l'eau (ISO 10705-3:2003)
L'ISO 10705-3:2003 spécifie les principes généraux qui permettent d'évaluer les performances des méthodes de concentration des bactériophages dans l'eau. La concentration est recommandée pour les échantillons d'eau supposés contenir moins de 3 pfp (particules formant plage) par millilitre. Les méthodes de concentration peuvent être appliquées à tous les types d'eau, sous réserve que la quantité et la nature des matières en suspension et/ou des matières dissoutes n'interfèrent pas avec le mode opératoire de concentration.
L'ISO 10705-3:2003 ne donne pas de détails spécifiques quant aux méthodes de concentration, mais esquisse les principes fondamentaux pour l'évaluation de l'adéquation d'une méthode particulière pour un type et un volume d'eau donnés. L'Annexe A donne des exemples de méthodes qui ont été jugées satisfaisantes ainsi que leur domaine d'application.
Kakovost vode - Ugotavljanje prisotnosti in števila bakteriofagov - 3. del: Validacija metod za koncentriranje bakteriofagov iz vode (ISO 10705-3:2003)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2025
Kakovost vode - Ugotavljanje prisotnosti in števila bakteriofagov - 3. del:
Validacija metod za koncentriranje bakteriofagov iz vode (ISO 10705-3:2003)
Water quality - Detection and enumeration of bacteriophages - Part 3: Validation of
methods for concentration of bacteriophages from water (ISO 10705-3:2003)
Wasserbeschaffenheit - Nachweis und Zählung von Bakteriophagen - Teil 3: Validierung
von Verfahren für die Konzentration von Bakteriophagen in Wasser (ISO 10705-3:2003)
Qualité de l'eau - Détection et dénombrement des bactériophages - Partie 3: Validation
des méthodes de concentration des bactériophages dans l'eau (ISO 10705-3:2003)
Ta slovenski standard je istoveten z: EN ISO 10705-3:2024
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 10705-3
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2024
EUROPÄISCHE NORM
ICS 07.100.20
English Version
Water quality - Detection and enumeration of
bacteriophages - Part 3: Validation of methods for
concentration of bacteriophages from water (ISO 10705-
3:2003)
Qualité de l'eau - Détection et dénombrement des Wasserbeschaffenheit - Nachweis und Zählung von
bactériophages - Partie 3: Validation des méthodes de Bakteriophagen - Teil 3: Validierung von Verfahren für
concentration des bactériophages dans l'eau (ISO die Konzentration von Bakteriophagen in Wasser (ISO
10705-3:2003) 10705-3:2003)
This European Standard was approved by CEN on 5 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10705-3:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 10705-3:2003 has been prepared by Technical Committee ISO/TC 147 "Water quality”
of the International Organization for Standardization (ISO) and has been taken over as EN ISO 10705-
3:2024 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2025, and conflicting national standards
shall be withdrawn at the latest by February 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 10705-3:2003 has been approved by CEN as EN ISO 10705-3:2024 without any
modification.
INTERNATIONAL ISO
STANDARD 10705-3
First edition
2003-10-01
Water quality — Detection and
enumeration of bacteriophages —
Part 3:
Validation of methods for concentration
of bacteriophages from water
Qualité de l'eau — Détection et dénombrement des bactériophages —
Partie 3: Validation des méthodes de concentration des bactériophages
dans l'eau
Reference number
ISO 10705-3:2003(E)
©
ISO 2003
ISO 10705-3:2003(E)
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ii © ISO 2003 — All rights reserved
ISO 10705-3:2003(E)
Contents Page
Foreword. iv
1 Scope. 1
2 Normative references. 1
3 Terms and definitions. 2
4 Principle. 2
5 Reagents. 2
6 Apparatus and glassware. 3
7 Sampling. 3
8 Preparation of sewage samples for spiking. 3
9 Procedure. 4
10 Calculation. 5
11 Analytical quality control . 6
12 Test report. 7
Annex A (informative) Recommended methods for concentration of bacteriophages from water
depending on the volume, turbidity and particle content. 8
Annex B (informative) Example of a validation process of a concentration method. 10
Bibliography . 13
ISO 10705-3:2003(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 10705-3 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods.
ISO 10705 consists of the following parts, under the general title Water quality — Detection and enumeration
of bacteriophages:
Part 1: Enumeration of F-specific RNA bacteriophages
Part 2: Enumeration of somatic coliphages
Part 3: Validation of methods for concentration of bacteriophages from water
Part 4: Enumeration of bacteriophages infecting Bacteroides fragilis
iv © ISO 2003 — All rights reserved
INTERNATIONAL STANDARD ISO 10705-3:2003(E)
Water quality — Detection and enumeration of
bacteriophages —
Part 3:
Validation of methods for concentration of bacteriophages from
water
WARNING — Persons using this part of ISO 10705 should be familiar with normal laboratory practice.
This part of ISO 10705 does not purport to address all of the safety problems, if any, associated with
its use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is imperative that personnel involved in validation of methods for concentration of
bacteriophages from water have relevant experience with the methods of enumeration of
[1]
bacteriophages (see ISO/TR 13843 ).
1 Scope
This part of ISO 10705 specifies the general principles for assessing the performance of methods for the
concentration of bacteriophages from water. Concentration is recommended for those water samples
expected to contain < 3 pfp (plaque-forming particles) per millilitre. Concentration methods can be applied to
all kinds of water provided that the amount and nature of suspended solids and/or dissolved matter do not
interfere with the concentration procedure.
This part of ISO 10705 does not give specific details of concentration methods, but outlines the fundamental
principles for evaluating the suitability of a particular method for a given type and volume of water. Annex A
gives examples of methods that have been found satisfactory and their fields of application.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial
suspension and decimal dilutions
ISO 8199, Water quality — General guide to the enumeration of micro-organisms by culture
ISO 10705-1, Water quality — Detection and enumeration of bacteriophages — Part 1: Enumeration of
F-specific RNA bacteriophages
ISO 10705-2, Water quality — Detection and enumeration of bacteriophages — Part 2: Enumeration of
somatic coliphages
ISO 10705-3:2003(E)
ISO 10705-4, Water quality — Detection and enumeration of bacteriophages — Part 4: Enumeration of
bacteriophages infecting Bacteroides fragilis
ISO/IEC Guide 2, Standardization and related activities — General vocabulary
3 Terms and definitions
For the
...
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