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This standard specifies a method for the detection, semi-quantitative and quantitative (MPN) enumeration of thermotolerant Campylobacter species. The method can be applied to all kinds of waters including: drinking water, ground water and well water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools, recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand and other sediments. This method can be used for the detection ...view more

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This document specifies a method for the detection, semi-quantitative and quantitative (MPN) enumeration of thermotolerant Campylobacter species. The method can be applied to all kinds of waters including: drinking water, ground water and well water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools, recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand and other sediments. This method can be used for the detection ...view more

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    • Standard
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This document specifies a method for the detection and quantification of Legionella spp. and
L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general
methodological requirements, performance evaluation requirements, and quality control requirements.
Technical details specified in this document are given for information only. Any other technical
solutions complying with the performance requirements are suitable.
NOTE 1 For performance requirements, see Claus...
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    • Technical specification
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    • Technical specification
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This document specifies requirements and gives guidance for performing the manipulations common
to each culture technique for the microbiological examination of water, particularly the preparation of
samples, culture media, and general apparatus and glassware, unless otherwise required in the specific
standard. It also describes the various techniques available for detection and enumeration by culture
and the criteria for determining which technique is appropriate.
This document is mainly i...
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This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements. Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable. NOTE 1 For performance requirements, see Clause 9. ...view more

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    • Technical specification
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    • Technical specification
      53 pages
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This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate.
This document is mainly inte...
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    • Standard
      66 pages
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This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate. This document is mainly intend...view more

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    • Standard
      56 pages
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      58 pages
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    • Standard
      7 pages
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    • Standard
      7 pages
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This document deals with characterization of microbiological methods. In terms of this document,
characterization means the study of parameters that can be measured to describe how the method is
likely to perform in a given set of conditions, which can be described as performance characteristics.
The document describes procedures for the determination of performance characteristics which can be
used for subsequent validation or verification of methods.
The emphasis is on selective quantitat...
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This International Standard specifies a method for the determination of the inhibition of the growth of the first fronds of Spirodela polyrhiza germinated from dormant turions, by substances and mixtures contained in water or waste water, including treated municipal waste water and industrial effluents.
The test is also applicable to pure chemicals and in particular plant protection products and pesticides.

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This document specifies culture methods for the isolation of Legionella and estimation of their numbers
in water samples.
These methods are applicable to all kinds of water samples including potable, industrial, waste and
natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.
Not all Legionella species are culturable; therefore, the methods described in this document do not
recover all species of Legionella.

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ISO 13843:2017 deals with characterization of microbiological methods. In terms of ISO 13843:2017, characterization means the study of parameters that can be measured to describe how the method is likely to perform in a given set of conditions, which can be described as performance characteristics. ISO 13843:2017 describes procedures for the determination of performance characteristics which can be used for subsequent validation or verification of methods.
The emphasis is on selective quantitat...
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ISO 13843:2017 deals with characterization of microbiological methods. In terms of ISO 13843:2017, characterization means the study of parameters that can be measured to describe how the method is likely to perform in a given set of conditions, which can be described as performance characteristics. ISO 13843:2017 describes procedures for the determination of performance characteristics which can be used for subsequent validation or verification of methods. The emphasis is on selective quantitati...view more

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    • Standard
      61 pages
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    • Standard
      62 pages
      French language

ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples.
These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.
Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.

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    • Standard
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This International Standard specifies a method for the enumeration of vegetative cells and spores of Clostridium perfringens by the membrane filtration method in samples of water intended for human consumption. However, the method can be applied to all types of water samples provided they do not contain particulate or colloidal matter that interferes with filtration.

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    • Standard
      20 pages
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ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples. These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc. Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.

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    • Standard
      38 pages
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    • Standard
      39 pages
      French language
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    • Standard
      1 page
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      1 page
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ISO 14189:2013 specifies a method for the enumeration of vegetative cells and spores of Clostridium perfringens by the membrane filtration method in samples of water intended for human consumption. However, the method can be applied to all types of water samples provided they do not contain particulate or colloidal matter that interferes with filtration.

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This European Standard specifies procedures for determining the ability of non-metallic materials to promote the growth of microorganisms.
This standard is applicable to those materials destined to be used under various conditions for the transport and storage of water intended for human consumption.
The Standard allows for the testing of a single type of material, or a product in which only one material is in contact with water. It is unsuitable for use with assembled products where more th...
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This European Standard specifies three methods for determining the ability of non-metallic materials to enhance the growth of micro-organisms.
This European Standard is applicable to those materials destined to be used under various conditions for the transport and storage of water intended for human consumption.
The standard allows for the testing of a single type of material, or a product in which only one material is in contact with water. It is unsuitable for use with assembled products wh...
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This part of ISO 9308 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform
bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform
agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity
of the differential agar medium, background growth can interfere with the reliable enumeration of
E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This...
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EN 16493 describes the most relevant rules of the Botanical and Zoological Codes necessary for unequivocal recording of biodiversity in the aquatic environment. Furthermore, guidance is given on how to deal with taxonomic changes in relation to recorded taxonomic names.

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ISO 9308-1:2014 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is...view more

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ISO 9308-1:2014 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is...view more

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    • Standard
      10 pages
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    • Standard
      10 pages
      English language
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    • Standard
      10 pages
      French language
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    • Standard
      10 pages
      French language

EN ISO 11133 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses. This Internation...view more

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    • Standard
      103 pages
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This European Standard describes the most relevant rules of the Botanical and Zoological Codes necessary for unequivocal recording of biodiversity in the aquatic environment. Furthermore, guidance is given on how to deal with taxonomic changes in relation to recorded taxonomic names.
NOTE   A Code only affects taxonomic changes carried out in the period covered by that particular edition of the Code.

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EN ISO 17994 specifies an evaluation procedure for comparing two methods intended for the quantification of the same target group or species of microorganisms. This International Standard provides the mathematical basis for the evaluation of the average relative performance of two quantitative methods against chosen criteria for the comparison. It does not provide data that would allow an assessment of the precision of the methods being compared. Precision of methods should be assessed as part o...view more

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EN ISO 9308-2 specifies a method for the enumeration of E. coli and coliform bacteria in water. The method is based on the growth of target organisms in a liquid medium and calculation of the “Most Probable Number” (MPN) of organisms by reference to MPN tables. This method can be applied to all types of water, including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. However, it must not be used for the enumeration of coliform bact...view more

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ISO 11133:2014 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses.
ISO 11133:201...
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    • Standard
      103 pages
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ISO 11133:2014 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses. ISO 11133:2014...view more

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    • Standard
      99 pages
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    • Standard
      99 pages
      English language
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    • Standard
      103 pages
      French language
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    • Standard
      103 pages
      French language

ISO 9308-2:2012 specifies a method for the enumeration of E. coli and coliform bacteria in water. The method is based on the growth of target organisms in a liquid medium and calculation of the "Most Probable Number" (MPN) of organisms by reference to MPN tables. This method can be applied to all types of water, including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. However it must not be used for the enumeration of coliform bac...view more

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    • Standard
      54 pages
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This International Standard specifies a method for the enumeration of vegetative cells and spores of Clostridium perfringens by the membrane filtration method in samples of water intended for human consumption. However, the method can be applied to all types of water samples provided they do not contain particulate or colloidal matter that interferes with filtration.

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    • Standard
      12 pages
      English language
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    • Standard
      17 pages
      English language
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    • Standard
      12 pages
      French language

ISO 17994:2014 specifies an evaluation procedure for comparing two methods with established performance characteristics according to ISO/TR 13843 and intended for the quantification of the same target group or species of microorganisms.
It provides the mathematical basis for the evaluation of the average relative performance of two quantitative methods against chosen criteria for the comparison. It does not provide data for assessment of the precision of the methods being compared. It is approp...
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    • Standard
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ISO 17994:2014 specifies an evaluation procedure for comparing two methods with established performance characteristics according to ISO/TR 13843 and intended for the quantification of the same target group or species of microorganisms. It provides the mathematical basis for the evaluation of the average relative performance of two quantitative methods against chosen criteria for the comparison. It does not provide data for assessment of the precision of the methods being compared. It is appropr...view more

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    • Standard
      22 pages
      English language
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    • Standard
      22 pages
      French language

ISO 14189:2013 specifies a method for the enumeration of vegetative cells and spores of Clostridium perfringens by the membrane filtration method in samples of water intended for human consumption. However, the method can be applied to all types of water samples provided they do not contain particulate or colloidal matter that interferes with filtration.

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    • Standard
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    • Standard
      12 pages
      French language

This part of ISO 9308 specifies a method for the enumeration of E. coli and coliform bacteria in water. The method is based on the growth of target organisms in a liquid medium and calculation of the "Most Probable Number" (MPN) of organisms by reference to MPN tables. This method can be applied to all types of water, including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. However, it must not be used for the enumeration of colif...view more

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    • Standard
      45 pages
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    • Standard
      51 pages
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    • Standard
      43 pages
      French language

This International Standard specifies a method for the detection of Salmonella spp. (presumptive or confirmed) in water samples. It is possible that, for epidemiological purposes or during outbreak investigations, other media are also required.

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ISO 19250:2010 specifies a method for the detection of Salmonella spp. (presumptive or confirmed) in water samples. It is possible that, for epidemiological purposes or during outbreak investigations, other media are also required.

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ISO 7827:2010 specifies a method for the evaluation of the “ready” and “ultimate” biodegradability of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, ISO 7827:2010 also gives specific definitions for the terms “ready” and “ultimate”.
The method applies to organic compounds which are: a) soluble at the concentration used under the conditions of the test [dissolved organic carbon (DOC) concentrations of 10 mg/l to 40 mg/l]; b) non‑volatile or havin...
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ISO 6341.2012 specifies a method for the determination of the acute toxicity to Daphnia magna Straus (Cladocera, Crustacea).
This method is applicable to: chemical substances which are soluble under the conditions of the test, or can be maintained as a stable suspension or dispersion under the conditions of the test; industrial or sewage effluents; treated or untreated waste water; aqueous extracts and leachates; fresh water (surface and ground water); eluates of fresh water sediment; pore wate...
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ISO 9308-2:2012 specifies a method for the enumeration of E. coli and coliform bacteria in water. The method is based on the growth of target organisms in a liquid medium and calculation of the "Most Probable Number" (MPN) of organisms by reference to MPN tables. This method can be applied to all types of water, including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. However it must not be used for the enumeration of coliform bac...view more

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    • Standard
      45 pages
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    • Standard
      51 pages
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    • Standard
      43 pages
      French language

This International Standard specifies a method for the detection of Salmonella spp. (presumptive or confirmed) in water samples. It is possible that, for epidemiological purposes or during outbreak investigations, other media are also required.
WARNING — It is possible that the method does not recover all Salmonella ser. Typhi and ser.
Paratyphi.
NOTE For a semi-quantitative approach, most probable number (MPN) tests can be performed

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    • Standard
      23 pages
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    • Standard
      31 pages
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    • Standard
      24 pages
      French language