ASTM E672-87(2006)
(Specification)Standard Specification for Disposable Glass Micropipets
Standard Specification for Disposable Glass Micropipets
ABSTRACT
This specification covers two different types of disposable glass micropipets, calibrated “to contain,” used in measuring microlitre volumes of liquids. The pipets made to these specifications shall be fabricated from borosilicate glass (Type I, Class A or B) or soda lime glass (Type II). Pipets shall be of one piece construction and shall meet the specified requirements for shape, dimensions, and capacity. The calibration line and identification markings such as color code marking are illustrated. The methods of reading and setting a liquid meniscus and a mercury meniscus are detailed. Capacity test shall be performed and calculation of micropipet volume shall be taken using the given formula.
SCOPE
1.1 This specification covers two different types of disposable micropipets, calibrated "to contain," used in measuring microlitre volumes of liquids.
1.2 The values stated in SI units are to be regarded as the standard.
1.3 The following precautionary caveat pertains only to paragraph 9.1.1 of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E672 – 87 (Reapproved 2006)
Standard Specification for
Disposable Glass Micropipets
This standard is issued under the fixed designation E672; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope 3.1.2 disposable micropipet—such micropipets will only be
expected to provide their specified performance during their
1.1 This specification covers two different types of dispos-
original use or operation.
able micropipets, calibrated “to contain,” used in measuring
microlitre volumes of liquids.
NOTE 1—The descriptions of “accuracy” and “repeatability” apply only
1.2 The values stated in SI units are to be regarded as the in cases where the distributions are Gaussian.
standard.
3.1.3 repeatability—theclosenessofagreementbetweenthe
1.3 The following precautionary caveat pertains only to
individual volumes obtained by applying the test procedure
paragraph 9.1.1 of this specification. This standard does not
specified in 9.4.2. It is quantified by the imprecision.
purport to address all of the safety concerns, if any, associated
with its use. It is the responsibility of the user of this standard
4. Classification
to establish appropriate safety and health practices and
4.1 This specification covers two different pipet designs as
determine the applicability of regulatory limitations prior to
follows:
use.
4.1.1 Type I—Disposable micropipets with calibration line
and color coding (see Fig. 1 and Table 1).
2. Referenced Documents
4.1.2 Type II—Disposable micropipets void of markings
2.1 ASTM Standards:
(see Fig. 2 and Table 2).
E438 Specification for Glasses in Laboratory Apparatus
NOTE 2—Type I pipets were originally specified by the Department of
2.2 ISO Standard:
Defense under MIL-P-36722.
R-1769 Color Coding for Pipets
5. Materials and Manufacture
3. Terminology
5.1 The pipets made to these specifications shall be fabri-
3.1 Definitions of Terms Specific to This Standard:
cated from borosilicate glass,Type I, ClassAor B or soda lime
3.1.1 accuracy—the closeness of agreement between the
glass, Type II, in accordance with Specification E438.
nominal value and the mean volume, obtained by applying the
test procedure specified in 9.4.1. It is quantified by the
6. Dimensions and Permissible Variations
inaccuracy of the mean (bias).
6.1 Design—Pipets shall be of one piece construction in
accordance with Table 1 and Table 2 for shape, dimensions,
and permissible variations. Any cross-section of the pipet,
This specification is under the jurisdiction of ASTM Committee E41 on
taken in a plane perpendicular to the longitudinal axis, shall be
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
Apparatus. circular.
Current edition approved Nov. 1, 2006. Published December 2006. Originally
6.2 Capacity—The pipet capacity shall be stated on the
´1
approved in 1978. Last previous edition approved in 2001 as E672 – 87 (2001) .
packagelabel,expressedasµL(microlitre);thisshallbeknown
DOI: 10.1520/E0672-87R06.
as the stated capacity, V , in making subsequent calculations.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
The expected deviation from the stated capacity shall be
Standards volume information, refer to the standard’s Document Summary page on
expressed as accuracy and coefficient of variation and shall be
the ASTM website.
3 tested for capacity as specified in 9.1. The unit, microlitre, µL,
Available fromAmerican National Standards Institute (ANSI), 25 W. 43rd St.,
4th Floor, New York, NY 10036, http://www.ansi.org. may be considered as equivalent to 0.001 cm .
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E672 – 87 (2006)
FIG. 1 Type I Pipet
TABLE 1 Dimensions for Type I Pipet
Maximum Volumetric Deviation
Stated Capacity, Minimum Di- Minimum Wall
Color Code
Coefficient of
µL ameter A, mm B, mm
Accuracy, %
Variation, %
5 white 1.0 0.35 61.0 <1.5
10 orange 1.0 0.25 60.5 <1.0
20 black 1.1 0.25 60.5 <1.0
25 2 white 1.1 0.25 60.5 <1.0
50 green 1.3 0.20 60.5 <1.0
100 blue 1.6 0.20 60.5 <1.0
200 red 2.2 0.20 60.5 <1.0
FIG.2TypeIIPipet
6.2.1 Accuracy (see4.1)—Theaccuracyshallbedetermined 6.4 Identification Markings:
as specified in 9.4 and shall be within the limits given in Table
6.4.1 Type I—The pipets in Fig. 1 shall be identified for
1 and Table 2.
capacity by a color code marking on each pipet consisting of
6.2.2 Coeffıcient of Variation (see section 4.2)—The coeffi-
one or two color bands (see Table 1). For a code consisting of
cient of variation shall be determined as specified in 9.4 and
one band, the band shall be from 2 to 6 mm wide; for a code
shall be within the limits given in Table 1 and Table 2.
consistingoftwobands,eachbandshallbe2to6mmwideand
6.3 Capacity Mark—Pipets in Fig. 1 shall have a capacity
separated with a space of 2 to 6 mm. The color code band, or
line that is calibrated “to contain” a volume of liquid at 20°C.
bands, shall completely encircle the pipet in a plane perpen-
The capacity line shall be 0.3 to 0.5 mm wide and shall
dicular to its longitudinal axis. The location of color band, or
completely encircle the pipet in a plane perpendicular to its
longitudinal axis.
E672 – 87 (2006)
TABLE 2 Dimensions for Type II Pipet
Maximum Volumetric Deviation
Stated Capacity, Minimum Minimum Di- Minimum Wall
Coefficient of
µL Length A, mm ameter B, mm C, mm
Accuracy, %
Variation, %
1 20 0.5 0.20 61.5 <2.0
2 20 0.5 0.20 61.2 <1.5
3 20 0.6 0.20 61.2 <1.5
4 20 0.6 0.20 61.2 <1.5
5 20 0.6 0.20 61.2 <1.5
10 20 0.6 0.10 61.0 <1.2
20 20 0.6 0.10 61.0 <1.2
25 30 0.6 0.10 61.0 <1.2
50 30 1.0 0.10 61.0 <1.2
100 50 1.3 0.10 61.0 <1.2
bands, shall be as specified in Fig. 1 with the selection of color, the approach of the meniscus from either above or below the
to designate capacity, according to ISO Recommendation line to its proper setting.
R-1769. 8.2 Reading a Mercury Meniscus (Type I only)—For all
6.4.2 Type II—Pipets in Fig. 2 are devoid of any markings
pipets,thereadingismadeatthehighestpointofthemeniscus.
and shall be identified for capacity on the package label. In order that the highest point may be observed, it is necessary
to place a shade of some light material immediately above and
7. Workmanship, Finish and Appearance
behind the meniscus, which renders the profile of the meniscus
7.1 The pipets in Fig. 1 and Fig. 2 shall be free of defects dark and clearly visible against a light background.
that will detract from their appearance or may impair their 8.2.1 Setting a Mercury Meniscus—Setting of the meniscus
serviceability. The pipets shall be free of significant foreign
shall be performed by one of the following methods.Wherever
matter, loose or embedded lint or chips that affect the bore, or practical, the meniscus should descend to the position of
stains when viewed under normal room lighting.
setting.
7.2 ThecalibrationlineandcolorcodeonTypeIpipetsshall 8.2.1.1 Method A—The position of the highest point of the
beappliedtotheglasspipetatlocationsspecifiedinFig.1.The
meniscus with reference to the graduation line is horizontally
calibration line shall be sufficiently deposited on the glass to tangent to the plane of the lower edge of the graduation line.
enable the setting of a meniscus and the color band shall be
The position of the meniscus is obtained by having the eye in
sufficiently deposited on the glass to identify the pipet as to its the same plane of the lower edge of the graduation line.
stated volume.
8.2.1.2 Method B—The position of the highest point of the
meniscus with reference to the graduation line is such that it is
8. Reading and Setting the Meniscus
in the plane of the middle of the graduation line. This position
8.1 Reading a Liquid Meniscus (Type I only)—For all of the meniscus is obtained by maki
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