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ASTM D5806-95(2017)

(Test Method)

Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration

Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration

SIGNIFICANCE AND USE
5.1 This test method is used to determine the percent actives in each type of the disinfectant quaternary ammonium salts, and also in the disinfectant products. Quaternary ammonium compounds being the active ingredients in disinfectant-type products require accurate determination to assess the cost and antimicrobial performance of such products.
SCOPE
1.1 This test method covers a potentiometric titration procedure for determining active matter in disinfectant quaternary ammonium salts. This test method is intended for the analysis of quaternary ammonium salts used as disinfectants, and only applies to the following commonly used quaternary ammonium salts: n-alkyldimethylbenzylammonium chloride (see Fig. 1), cetyltrimethylammonium chloride, and a blend of n-octyldecyl dimethylammonium chloride, di-n-octyl dimethylammonium chloride, and di-n-decyldimethyl ammonium chloride (see Fig. 2). Also, this test method can be applied to the analysis of disinfectant type products where the formula ingredients are known and the quaternary ammonium salt is one of the above. Interferences such as amines oxides and betaines present in disinfectant formulations were not tested.  X−  =  chloride, and    R  =  aliphatic, normal C8 − C22.    
1.2 This disinfectant quaternary ammonium salt conforms to the structures in Fig. 1 and Fig. 2.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautionary information, see Section 8.

General Information

Status
Published
Publication Date
31-Dec-2016
ICS
71.100.35 - Chemicals for industrial and domestic disinfection purposes
Technical Committee
D12 - Soaps and Other Detergents
Drafting Committee
D12.12 - Analysis and Specifications of Soaps, Synthetics, Detergents and their Components
Current Stage
Ref Project

Relations

Refers
ASTM D459-16 - Standard Terminology Relating to Soaps and Other Detergents
Effective Date
01-Jul-2016
Refers
ASTM D3049-89(2016) - Standard Test Method for Synthetic Anionic Ingredient by Cationic Titration
Effective Date
01-Jul-2016

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ASTM D5806-95(2017) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric TitrationASTM D5806-95(2017) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration
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ASTM D5806-95(2017) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D5806 − 95 (Reapproved 2017)
Standard Test Method for
Disinfectant Quaternary Ammonium Salts by Potentiometric
1
Titration
This standard is issued under the fixed designation D5806; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2
2.1 ASTM Standards:
1.1 This test method covers a potentiometric titration pro-
D459Terminology Relating to Soaps and Other Detergents
cedure for determining active matter in disinfectant quaternary
D1193Specification for Reagent Water
ammonium salts. This test method is intended for the analysis
D1681Test Method for SyntheticAnionicActive Ingredient
of quaternary ammonium salts used as disinfectants, and only
in Detergents by Cationic Titration Procedure
appliestothefollowingcommonlyusedquaternaryammonium
D3049Test Method for Synthetic Anionic Ingredient by
salts: n-alkyldimethylbenzylammonium chloride (see Fig. 1),
Cationic Titration
cetyltrimethylammoniumchloride,andablendof n-octyldecyl
E180Practice for Determining the Precision of ASTM
dimethylammonium chloride, di-n-octyl dimethylammonium
Methods for Analysis and Testing of Industrial and Spe-
chloride,anddi-n-decyldimethylammoniumchloride(seeFig. 3
cialty Chemicals (Withdrawn 2009)
2). Also, this test method can be applied to the analysis of
disinfectant type products where the formula ingredients are
3. Terminology
known and the quaternary ammonium salt is one of the above.
3.1 Definitions—See Terminology D459.
Interferences such as amines oxides and betaines present in
disinfectant formulations were not tested.
4. Summary of Test Method
1.2 This disinfectant quaternary ammonium salt conforms 4.1 Disinfectant type quaternary ammonium compounds
to the structures in Fig. 1 and Fig. 2. present,astheactivematerialsindisinfectanttypeproductsare
titrated potentiometrically in an aqueous medium with a
1.3 The values stated in SI units are to be regarded as
standard solution of sodium lauryl sulphate using a nitrate
standard. No other units of measurement are included in this
ion-selective electrode or a surfactant electrode (see also Test
standard.
Method D1681). In this potentiometric titration, the reaction
involves the formation of a complex between the disinfectant
1.4 This standard does not purport to address all of the
quaternary ammonium compound and the anionic surfactant
safety concerns, if any, associated with its use. It is the
which then precipitates. At the end point, the nitrate ion
responsibility of the user of this standard to establish appro-
electrode or surfactant electrode appears to respond to an
priate safety and health practices and determine the applica-
excess of titrant with a potential change large enough to give a
bility of regulatory limitations prior to use. For specific
well defined inflection in the titration curve.
precautionary information, see Section 8.
5. Significance and Use
5.1 Thistestmethodisusedtodeterminethepercentactives
in each type of the disinfectant quaternary ammonium salts,
1 2
This test method is under the jurisdiction ofASTM Committee D12 on Soaps For referenced ASTM standards, visit the ASTM website, www.astm.org, or
and Other Detergents and is the direct responsibility of Subcommittee D12.12 on contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
AnalysisandSpecificationsofSoaps,Synthetics,DetergentsandtheirComponents. Standards volume information, refer to the standard’s Document Summary page on
Current edition approved Jan. 1, 2017. Published February 2017. Originally the ASTM website.
3
approvedin1995.Lastpreviouseditionapprovedin2009asD5806-95(2009).DOI: The last approved version of this historical standard is referenced on
10.1520/D5806-95R17. www.astm.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D5806 − 95 (2017)
where:
−
X = chloride, and
R = aliphatic, normal C −C .
8 22
FIG. 1n-alkyldimethylbenzylammonium chloride and n-alkyldimethylethylbenzylammonium chloride
NOTE 2—Other electrodes (for example, calomel electrodes) are suit-
able as the reference electrode provided they give a stable reference
potential during the titration. Reference electrodes having a ceramic or an
asbestos junction tend to clog with use. Therefore, a ground-glass sleeve
12
electrode is suggested.
7. Reagents
where: 13
7.1 Sodium Lauryl Sulf
...

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1.1 This test method is used to evaluate the antimicrobial efficacy of sanitizers on precleaned, inanimate, hard, nonporous, non-food contact surfaces against Staphylococcus aureus, or Klebsiella pneumoniae or Klebsiella aerogenes, or a combination thereof. Appropriate modifications to the method may be required when testing organisms not specified herein. When utilizing test surfaces not described herein (see Test Method E2274) or when evaluating spray-based or towelette-based antimicrobial products, modifications may also be required.  
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1.2 Knowledge of microbiological and statistical techniques is required for these procedures.
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5.1 The Single Tube Method is designed to evaluate the efficacy of disinfectants against biofilm grown in the CDC biofilm reactor following the procedures outlined in Practice E3161. Biofilm grown in the CDC reactor is representative of biofilm that forms under high fluid shear on surfaces conducive to biofilm formation.  
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Note 2: Application of viruses on the entire surface of both hands entails a greater risk to the subjects than using fingerpads only. Therefore, greater care is needed to ensure that the hands of the participants are free from any apparent damage. Also, virus preparations must be thoroughly screened for, or documented to be free from, extraneous or adventitious pathogens before use in such tests.
SCOPE
1.1 This test method is designed to evaluate handwash or handrub agents for their ability to reduce or eliminate viable viruses from the skin of human hands.
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1.3 This standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. The user should consult a reference for laboratory safety recommendations. (3-5)  
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SCOPE
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5.2.3.2 Results presented in Refs (1, 3) (and currently unpublished results) indicate that these media, combined with the test temperatures and conditions described herein will generate results with a high level of practical confidence for detecting culturable Bacillus spores.  
5.2.4 Safety—Inoculated coupons are contained within filter units.  
5.2.5 Simplicity of Testing—Tests and extractions are performed in the same filter unit to minimize coupon handling steps.  
5.2.6 Scalable and Rapid—A maximum of 36 samples can be processed in 1 h by two technicians; a total of 300 samples have been processed by six technicians in 5 h (1, 2).  
5.2.7 Wide application for numerous Bacillus species and strains. The method has also been modified and used for vegetative bacteria and viruses as well (1, 2).
SCOPE
1.1 This practice is used to quantify the efficacy of liquid or solid decontaminants on Bacillus spores dried on the surface of coupons made from porous and non-porous materials. This practice can distinguish between bactericidal and bacteriostatic chemicals within decontamination mixtures. This is important because many decontaminants contain both reactive compounds and high concentrations of bacteriostatic surfactants. All test samples are directly compared to pre-neutralized controls, un-inoculated negative growth controls, and solution controls on the same day as the test in order to increase practical confidence in the inactivation data.  
1.2 This procedure should be performed only by those trained in microbiological techniques, are familiar with antimicrobial (sporicidal) agents and the application instructions of the antimicrobial products.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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