ASTM E934-94(2015)

Standard Specification for Serological Pipet, Disposable Plastic

ABSTRACT
This specification covers disposable plastic serological pipets, calibrated to deliver when measuring volumes of liquids. The pipets shall be fabricated from crystal-grade, uncolored polystyrene, or its regrind. They shall adhere to the property requirements discussed herein for design (shape, delivery tips, top end, dimensions, and outflow times), graduation and identification markings, capacity, and accuracy and coefficient of variation.
SCOPE
1.1 This specification covers disposable plastic serological pipets, calibrated “to deliver” when measuring volumes of liquids.  
1.1.1 Any institution or individual who reuses a disposable pipet must bear full responsibility for its safety and effectiveness.

General Information

• Status: Historical
• Publication Date: 31-May-2015
• ICS: 11.100 - Laboratory medicine
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E934 −94 (Reapproved 2015)
Standard Specification for
1
Serological Pipet, Disposable Plastic
This standard is issued under the fixed designation E934; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the U.S. Department of Defense.
1. Scope 4. Material and Manufacturer
1.1 This specification covers disposable plastic serological 4.1 The pipets made to this specification shall be fabricated
pipets, calibrated “to deliver” when measuring volumes of from crystal grade, uncolored polystyrene, or regrind of same,
liquids. in accordance with Specification D703.
1.1.1 Any institution or individual who reuses a disposable
pipet must bear full responsibility for its safety and effective- 5. Design
3
ness.
5.1 Shape—0.5,1.0,and2.0-cm pipetsshallbestraightand
of one-piece construction.Any cross section of a pipet taken in
2. Referenced Documents
a plane perpendicular to the longitudinal axis shall be circular.
2
3
2.1 ASTM Standards:
5.1.1 Pipetsof5.0,10.0,25.0,and50.0cm shallbestraight
D703 Specification for Polystyrene Molding and Extrusion
andmayconsistofone,two,orthreecomponents,theextruded
3
Materials (Withdrawn 0)
pipet barrel, the pulled or injection molded tip, and the plain,
E542 Practice for Calibration of Laboratory Volumetric
pulled, or injection molded top end. Any cross section of a
Apparatus
pipet taken in a plane perpendicular to the longitudinal axis
E920 Specification for Commercially Packaged Laboratory
shall be circular.
Apparatus
5.2 Delivery Tips—Delivery tips shall be made with a
E921 Specification for Export Packaged Laboratory Appa-
gradual taper of 10 to 40 mm. The tip end shall be reasonably
ratus
perpendicular to the longitudinal axis of the pipet, and shall be
E1133 Practice for Performance Testing of Packaged Labo-
free of internal flash.
ratory Apparatus for United States Government Procure-
3
ments 5.3 TopEnd—The5,10,25.0,and50.0-cm sizesshallhave
a top end with an inside diameter of 2 to 6.5 mm for a
3. Terminology
minimumdistanceof20mmfromtheopenend,andshallhave
anoveralllengthof24to28mm.Onallsizes,thetopendshall
3.1 Definitions of Terms Specific to This Standard:
besuitableforpluggingwithfilteringmaterial.Thetopmaybe
3.1.1 accuracy—the expected distribution of mean volumes
flat or chamfered and must be without sharp outer edges. All
around the stated volume.
top ends shall be reasonably perpendicular to the longitudinal
3.1.2 coeffıcient of variation—the expected distribution of
axis of the pipet. The O.D. of top end should range from 7 to
individual volumes around the mean volume.
9 mm.
3.1.3 disposable pipet—such pipets will only be expected to
5.4 Dimensions and Outflow Times—The limiting dimen-
provide their specified performance during their original use or
sions and outflow times shall be as shown in Table 1. Outflow
operation.
times shall be determined by means of a stopwatch on
unplugged pipets when using distilled water at 25 6 5°C.
1
This specification is under the jurisdiction of ASTM Committee E41 on
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
6. Markings
Laboratory Ware and Supplies.
CurrenteditionapprovedJune1,2015.PublishedJuly2015.Originallyapproved
6.1 Graduation Markings—Graduation lines shall be be-
in 1983. Last previous edition approved in 2009 as E934 – 94 (2009). DOI:
tween 0.2 mm and 0.5 mm in width, and in a plane perpen-
10.1520/E0934-94R15.
2
dicular to the longitudinal axis of the pipet parallel to each
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
other.Amaingraduationlineshallextendatleast ⁄5oftheway
Standards volume information, refer to the standard’s Document Summary page on
around the pipet. The values of all main graduation lines shall
the ASTM website.
3
be in Arabic numbers directly above the lines referenced.
The last approved version of this historical standard is referenced on
1
www.astm.org. Intermediategraduationlinesshallextendatleast ⁄5oftheway
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E934−94 (2015)
TABLE 1 Dimensions and Outflow Times
3
Least Value Graduation cm (mL) Outflow, s
Outside Diameter Wall Thickness,
3
Capacity, cm
Graduated Portion, mm min, mm
Main No. Graduation 0 to min max
0.5 0.01 0.1 0.4 4.5 to 4.8 0.5 3.0 0.8
1.0 0.01 0.1 0.9 4.5 to 4.8 0.5 3.0 0.8
1.0 0.10 0.1 0.9 4.5 to 4.8 0.5 3.0 0.8
2.0 0.01 0
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E934 − 94 (Reapproved 2009) E934 − 94 (Reapproved 2015)
Standard Specification for
1
Serological Pipet, Disposable Plastic
This standard is issued under the fixed designation E934; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the U.S. Department of Defense.
1. Scope
1.1 This specification covers disposable plastic serological pipets, calibrated “to deliver” when measuring volumes of liquids.
1.1.1 Any institution or individual who reuses a disposable pipet must bear full responsibility for its safety and effectiveness.
Any institution or individual who reuses a disposable pipet must bear full responsibility for its safety and effectiveness.
2. Referenced Documents
2
2.1 ASTM Standards:
3
D703 Specification for Polystyrene Molding and Extrusion Materials (Withdrawn 0)
E542 Practice for Calibration of Laboratory Volumetric Apparatus
E920 Specification for Commercially Packaged Laboratory Apparatus
E921 Specification for Export Packaged Laboratory Apparatus
E1133 Practice for Performance Testing of Packaged Laboratory Apparatus for United States Government Procurements
3. Terminology
3.1 Definitions of Terms Specific to This Standard:
3.1.1 accuracy—the expected distribution of mean volumes around the stated volume.
3.1.2 coeffıcient of variation—the expected distribution of individual volumes around the mean volume.
3.1.3 disposable pipet—such pipets will only be expected to provide their specified performance during their original use or
operation.
4. Material and Manufacturer
4.1 The pipets made to this specification shall be fabricated from crystal grade, uncolored polystyrene, or regrind of same, in
accordance with Specification D703.
5. Design
3
5.1 Shape—0.5, 1.0, and 2.0-cm pipets shall be straight and of one-piece construction. Any cross section of a pipet taken in
a plane perpendicular to the longitudinal axis shall be circular.
3
5.1.1 Pipets of 5.0, 10.0, 25.0, and 50.0 cm shall be straight and may consist of one, two, or three components, the extruded
pipet barrel, the pulled or injection molded tip, and the plain, pulled, or injection molded top end. Any cross section of a pipet taken
in a plane perpendicular to the longitudinal axis shall be circular.
5.2 Delivery Tips—Delivery tips shall be made with a gradual taper of 10 to 40 mm. The tip end shall be reasonably
perpendicular to the longitudinal axis of the pipet, and shall be free of internal flash.
3
5.3 Top End—The 5, 10, 25.0, and 50.0-cm sizes shall have a top end with an inside diameter of 2 to 6.5 mm for a minimum
distance of 20 mm from the open end, and shall have an overall length of 24 to 28 mm. On all sizes, the top end shall be suitable
1
This specification is under the jurisdiction of ASTM Committee E41 on Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
ApparatusLaboratory Ware and Supplies.
Current edition approved Nov. 1, 2009June 1, 2015. Published December 2009July 2015. Originally approved in 1983. Last previous edition approved in 20042009 as
E934 – 94 (2004).(2009). DOI: 10.1520/E0934-94R09.10.1520/E0934-94R15.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
The last approved version of this historical standard is referenced on www.astm.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E934 − 94 (2015)
for plugging with filtering material. The top may be flat or chamfered and must be without sharp outer edges. All top ends shall
be reasonably perpendicular to the longitudinal axis of the pipet. The O.D. of top end should range from 7 to 9 mm.
5.4 Dimensions and Outflow Times—The limiting dimensions and outflow times shall be as shown in Table 1. Outflow times
shall be determined by means of a stopwatch on unplugged pipets when using distilled water at 25 6 5°C.
6. Markings
6.1 Graduation Markings—Graduation lines shall be between 0.2 mm and 0.5 mm in width, and in a plane perpendicular to the
3
longitudinal axis of the pipet paralle
...