ASTM E1517-99(2006)
(Test Method)Standard Test Method for Determining the Effectiveness of Liquid, Gel, Cream, or Shampoo Insecticides Against Human Louse Ova
Standard Test Method for Determining the Effectiveness of Liquid, Gel, Cream, or Shampoo Insecticides Against Human Louse Ova
SIGNIFICANCE AND USE
This test method is a standardized test for the gathering of efficacy data for human louse ovicides.
Data collection in this manner is suitable for product development and comparison, and for review by regulatory agencies, to support the registration of human louse ovicidal products.
SCOPE
1.1 This test method determines the effectiveness of ovicidal materials in liquid, gel, cream, or shampoo form against the ova (that is, eggs or nits) of the human louse. Pediculus humanus.
1.2 This test method is intended for use by those wishing to develop efficacy data or compare ovicidal formulations for human louse ova control.
1.3 This test method consists of five replicates for a statistical comparison of formulations.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E1517 − 99(Reapproved 2006)
Standard Test Method for
Determining the Effectiveness of Liquid, Gel, Cream, or
Shampoo Insecticides Against Human Louse Ova
This standard is issued under the fixed designation E1517; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.3 Percenteggmortality,correctedbyAbbott’sFormula,is
determined.
1.1 This test method determines the effectiveness of ovici-
dalmaterialsinliquid,gel,cream,orshampooformagainstthe
4. Significance and Use
ova (that is, eggs or nits) of the human louse, Pediculus
humanus.
4.1 This test method is a standardized test for the gathering
of efficacy data for human louse ovicides.
1.2 This test method is intended for use by those wishing to
develop efficacy data or compare ovicidal formulations for
4.2 Data collection in this manner is suitable for product
human louse ova control.
development and comparison, and for review by regulatory
agencies, to support the registration of human louse ovicidal
1.3 This test method consists of five replicates for a statis-
products.
tical comparison of formulations.
1.4 The values stated in SI units are to be regarded as the
5. Apparatus and Materials
standard. The values given in parentheses are for information
5.1 Applicators—Egg-infested hairs are attached to the end
only.
ofawoodenapplicatorstickwithducttapesuchthat30nitsare
1.5 This standard does not purport to address all of the
on 1 to 3 hairs. Each replicate of 30 eggs is examined under a
safety concerns, if any, associated with its use. It is the
dissecting microscope to confirm viability. Any eggs that are
responsibility of the user of this standard to establish appro-
shrunken or with other indications of being nonviable are
priate safety and health practices and determine the applica-
excluded.
bility of regulatory limitations prior to use.
5.2 Beakers—A100-mLbeaker is used to contain 60 mLof
2. Terminology
test ovicide and another to contain 60 mL of water (control),
into which the applicators are dipped. A 1000-mL beaker is
2.1 Definitions of Terms Specific to This Standard:
2.1.1 hatched—those eggs (nits) from which the nymph has used for washing the eggs.
emerged completely. An empty, clear egg case with the
5.3 Heating Surface—A slide dryer that provides heat of
operculum clearly open.
approximately 32°C (90°F).
2.1.2 unhatched—those eggs that are opaque. The opercu-
5.4 Incubator, capable of maintaining a temperature of 31.7
lum is closed or the nymph is partly emerged.
6 0.5°C (89°F) and a relative humidity of 60 6 10%.
3. Summary of Test Method
5.5 Water Bath, capable of maintaining a temperature of
32°C (90°F).
3.1 Five replicates of 30 eggs are immersed in a test
compound for a set period of time, washed, rinsed, blotted,
5.6 Wash Bottle, Stop Watch, and Dissecting Scope.
dried, and incubated.
5.7 Test Insect—The human louse, Pediculus humanus.
3.2 Five control replicates are attached to human hair and
5.8 Positive Control Treatment—Sixty mL of solution
processed as the treatment replicates, but with immersion in
known to give 65 to 95% mortality of louse eggs when used
water.
under these test conditions.
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
responsibility of Subcommittee E35.12 on Insect Control Agents. Astrain of the human body louse, Pediculus humanus, is maintained by Insect
Current edition approved April 1, 2006. Published April 2006. Originally Control and Research, Inc., Baltimore, MD 21228-1199.The strain was established
approved in 1993. Last previous edition approved in 2005 as E1517–99 (2005). from a U.S. Department ofAgriculture Gainesville colony. It is a susceptible strain
DOI: 10.1520/E1517-99R06. and, through selection, has been adapted to the New Zealand White rabbit.
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