ASTM E1517-99(2005)

Standard Test Method for Determining the Effectiveness of Liquid, Gel, Cream, or Shampoo Insecticides Against Human Louse Ova

SCOPE
1.1 This test method determines the effectiveness of ovicidal materials in liquid, gel, cream, or shampoo form against the ova (that is, eggs or nits) of the human louse. Pediculus humanus.
1.2 This test method is intended for use by those wishing to develop efficacy data or compare ovicidal formulations for human louse ova control.
1.3 This test method consists of five replicates for a statistical comparison of formulations.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 31-Oct-2005
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents

Relations

• Replaces: ASTM E1517-99 - Standard Test Method for Determining the Effectiveness of Liquid, Gel, Cream, or Shampoo Insecticides Against Human Louse OVA
• Effective Date: 01-Nov-2005
• Replaced By: ASTM E1517-99(2006) - Standard Test Method for Determining the Effectiveness of Liquid, Gel, Cream, or Shampoo Insecticides Against Human Louse Ova
• Effective Date: 01-Apr-2006

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E1517–99 (Reapproved 2005)
Standard Test Method for
Determining the Effectiveness of Liquid, Gel, Cream, or
Shampoo Insecticides Against Human Louse Ova
This standard is issued under the fixed designation E 1517; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 4. Significance and Use
1.1 This test method determines the effectiveness of ovi- 4.1 This test method is a standardized test for the gathering
cidal materials in liquid, gel, cream, or shampoo form against of efficacy data for human louse ovicides.
the ova (that is, eggs or nits) of the human louse. Pediculus 4.2 Data collection in this manner is suitable for product
humanus. development and comparison, and for review by regulatory
1.2 This test method is intended for use by those wishing to agencies, to support the registration of human louse ovicidal
develop efficacy data or compare ovicidal formulations for products.
human louse ova control.
5. Apparatus and Materials
1.3 This test method consists of five replicates for a statis-
tical comparison of formulations. 5.1 Applicators—Egg-infested hairs are attached to the end
ofawoodenapplicatorstickwithducttapesuchthat30nitsare
1.4 The values stated in SI units are to be regarded as the
standard. The values given in parentheses are for information on 1 to 3 hairs. Each replicate of 30 eggs is examined under a
dissecting microscope to confirm viability. Any eggs that are
only.
1.5 This standard does not purport to address all of the shrunken or with other indications of being nonviable are
excluded.
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro- 5.2 Beakers—A100-mLbeaker is used to contain 60 mLof
test ovicide and another to contain 60 mL of water (control),
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use. into which the applicators are dipped. A 1000-mL beaker is
used for washing the eggs.
2. Terminology
5.3 Heating Surface—A slide dryer that provides heat of
2.1 Definitions of Terms Specific to This Standard: approximately 32°C (90°F).
2.1.1 hatched—those eggs (nits) from which the nymph has 5.4 Incubator, capable of maintaining a temperature of 31.7
emerged completely. An empty, clear egg case with the 6 0.5°C (89°F) and a relative humidity of 60 6 10 %.
operculum clearly open. 5.5 Water Bath, capable of maintaining a temperature of
2.1.2 unhatched—those eggs that are opaque. The opercu- 32°C (90°F).
lum is closed or the nymph is partly emerged. 5.6 Wash Bottle, Stop Watch, and Dissecting Scope.
5.7 Test Insect—The human louse, Pediculus humanus.
3. Summary of Test Method
5.8 Positive Control Treatment—Sixty mL of solution
3.1 Five replicates of 30 eggs are immersed in a test
known to give 65 – 95 % mortality of louse eggs when used
compound for a set period of time, washed, rinsed, blotted, under these test conditions.
dried, and incubated.
6. Rearing of Test Insects
3.2 Five control replicates are attached to human hair and
processed as the treatment replicates, but with immersion in 6.1 The adult human lice are blood fed on the shaven belly
water. of a restrained rabbit.
3.3 Percent egg mortality, corrected byAbbott’s Formula, is 6.2 The lice are transferred to human hair cuttings, held in
determined. a petri dish, and incubated for 24 h for oviposition to occur.
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides and Alternative Control Agents and is the direct responsibility of Astrain of the human body louse, Pediculus humanus, is maintained by Insect
Subcommittee E35.22 on Pesticide Formulations and Delivery Systems. Control and Research, Inc., Baltimore, MD 21228-1199. The strain was established
Current edition approved Nov. 1, 2005. Published November 2005. Originally from a U.S. Department ofAgriculture Gainesville colony. It is a susceptible strain
approved in 1993. Last previous edition
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