ASTM D5176-20

Standard Test Method for Total Chemically Bound Nitrogen in Water by Pyrolysis and Chemiluminescence Detection

SIGNIFICANCE AND USE
5.1 This test method is useful for the determination of total chemically bound nitrogen in wastewaters and other waters.
SCOPE
1.1 This test method covers the determination of the total nitrogen content of water in concentrations from 0.5 to 1000 mg/L. Higher nitrogen concentrations may be determined by making the proper dilutions.  
1.2 This test method does not determine molecular nitrogen (N2).  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 14-Jul-2020
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: D19 - Water
• Drafting Committee: D19.06 - Methods for Analysis for Organic Substances in Water

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D5176 − 20
Standard Test Method for
Total Chemically Bound Nitrogen in Water by Pyrolysis and
1
Chemiluminescence Detection
This standard is issued under the fixed designation D5176; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Terminology
3.1 Definitions:
1.1 This test method covers the determination of the total
3.1.1 For definitions of terms used in this standard, refer to
nitrogen content of water in concentrations from 0.5 to 1000
Terminology D1129.
mg/L. Higher nitrogen concentrations may be determined by
making the proper dilutions.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 total chemically bound nitrogen, n—all inorganic and
1.2 This test method does not determine molecular nitrogen
organicnitrogeninthesample,exceptmolecularnitrogen(N ).
2
(N ).
2
4. Summary of Test Method
1.3 The values stated in SI units are to be regarded as
standard. No other units of measurement are included in this
4.1 The sample of water is introduced into a stream of
standard.
oxygen or inert/oxygen mix flowing through a quartz pyrolysis
tube. Oxidative pyrolysis converts chemically bound nitrogen
1.4 This standard does not purport to address all of the
to nitric oxide (NO). The gas stream is dried and the NO is
safety concerns, if any, associated with its use. It is the
contacted with ozone (O ) producing metastable nitrogen
3
responsibility of the user of this standard to establish appro-
dioxide (NO *). As the NO * decays, light is emitted and
priate safety, health, and environmental practices and deter- 2 2
detected by a photomultiplier tube. The resulting signal is a
mine the applicability of regulatory limitations prior to use.
measure of the total chemically bound nitrogen in the sample.
1.5 This international standard was developed in accor-
dance with internationally recognized principles on standard-
5. Significance and Use
ization established in the Decision on Principles for the
5.1 This test method is useful for the determination of total
Development of International Standards, Guides and Recom-
chemically bound nitrogen in wastewaters and other waters.
mendations issued by the World Trade Organization Technical
3
Barriers to Trade (TBT) Committee. 6. Apparatus
6.1 Pyrolysis Furnace—An electric tube furnace capable of
2. Referenced Documents
achieving a temperature of 1100°C. The furnace may be single
2
or multizoned and may have common or separate and inde-
2.1 ASTM Standards:
pendent temperature controls.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
6.2 Pyrolysis Tube—The pyrolysis tube must be fabricated
D2777 Practice for Determination of Precision and Bias of
from quartz and should be designed to ensure complete
Applicable Test Methods of Committee D19 on Water
pyrolysis of a wide variety of samples.
D5847 Practice for Writing Quality Control Specifications
6.3 Chemiluminescence Detector—Thedetectorshallhavea
for Standard Test Methods for Water Analysis
photomultiplier tube capable of sensing the light emission of
the decaying NO *. The detector shall have digital display,
2
onboard ozone generator and analog output for data system or
1
This test method is under the jurisdiction of ASTM Committee D19 on Water
strip chart recorder.
andisthedirectresponsibilityofSubcommitteeD19.06onMethodsforAnalysisfor
6.4 Recorder (optional)—The recorder shall be able to
Organic Substances in Water.
Current edition approved July 15, 2020. Published July 2020. Originally
accept a 1 V full scale signal and to provide a chart speed of 1
approved in 1991. Last previous edition approved in 2015 as D5176 – 08 (2015).
cm/min.
DOI: 10.1520/D5176-20.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM The apparatus described in 6.1 – 6.7 is manufactured by Antek Instruments,
Standards volume information, refer to the standard’s Document Summary page on Inc., Houston, TX, and Dohrmann Division of Rosemount Analytical Inc., Santa
the ASTM website. Clara, CA, and was used in the validation study of this test method.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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D5176 − 20
6.5 Microlitre Syringe—Any standard series of microlitre 9.3 Syringe Injection—Fill the syringe to the 5 µLmark an
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D5176 − 08 (Reapproved 2015) D5176 − 20
Standard Test Method for
Total Chemically Bound Nitrogen in Water by Pyrolysis and
1
Chemiluminescence Detection
This standard is issued under the fixed designation D5176; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method covers the determination of the total nitrogen content of water in concentrations from 0.5 to 1000 mg/L.
Higher nitrogen concentrations may be determined by making the proper dilutions.
1.2 This test method does not determine molecular nitrogen (N ).
2
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.5 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water
D5847 Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
3. Terminology
3.1 Definitions:
3.1.1 For definitions of terms used in this standard, refer to Terminology D1129.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 total chemically bound nitrogen, n—all inorganic and organic nitrogen in the sample, except molecular nitrogen (N ).
2
4. Summary of Test Method
4.1 The sample of water is introduced into a stream of oxygen or inert/oxygen mix flowing through a quartz pyrolysis tube.
Oxidative pyrolysis converts chemically bound nitrogen to nitric oxide (NO). The gas stream is dried and the NO is contacted with
ozone (O ) producing metastable nitrogen dioxide (NO *). As the NO * decays, light is emitted and detected by a photomultiplier
3 2 2
tube. The resulting signal is a measure of the total chemically bound nitrogen in the sample.
5. Significance and Use
5.1 This test method is useful for the determination of total chemically bound nitrogen in wastewaters and other waters.
1
This test method is under the jurisdiction of ASTM Committee D19 on Water and is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
Organic Substances in Water.
Current edition approved June 15, 2015July 15, 2020. Published June 2015July 2020. Originally approved in 1991. Last previous edition approved in 20082015 as
D5176 – 08.D5176 – 08 (2015). DOI: 10.1520/D5176-08R15.10.1520/D5176-20.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’sstandard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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D5176 − 20
3
6. Apparatus
6.1 Pyrolysis Furnace—An electric tube furnace capable of achieving a temperature of 1100°C. The furnace may be single or
multizoned and may have common or separate and independent temperature controls.
6.2 Pyrolysis Tube—The pyrolysis tube must be fabricated from quartz and should be designed to ensure complete pyrolysis of
a wide variety of samples.
6.3 Chemiluminescence Detector—The detector shall have a photomultiplier tube capable of sensing the light emission of the
decaying NO *. The detector shall have digital display, onboard ozone generator and analog output for data system or strip chart
2
recorder.
6.4 Recorder (optional)—The recorder shall be able to accept a 1 V full scale signal and to provide a chart speed of 1 cm/min.
6.5 Microlitre Syringe—Any standard series of microlitre syringes with stainless steel needles
...