ASTM E714-94(2015)

Standard Specification for Disposable Glass Serological Pipets

ABSTRACT
This specification covers disposable glass serological pipets, calibrated "to deliver," used in measuring volumes of liquids. Pipets shall be straight and have one-piece construction, fabricated from borosilicate glass, Type I, Class A or B; or soda-lime glass, Type II. Any cross section of a pipet taken in a plane perpendicular to the longitudinal axis shall be circular. The accuracy and coefficient of variation of the pipets shall be tested gravimetrically.
SCOPE
1.1 This specification covers disposable glass serological pipets, calibrated “to deliver,” used in measuring volumes of liquids.

General Information

• Status: Historical
• Publication Date: 31-May-2015
• ICS: 11.100 - Laboratory medicine
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E714 −94 (Reapproved 2015)
Standard Specification for
1
Disposable Glass Serological Pipets
This standard is issued under the fixed designation E714; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5. Design
1.1 This specification covers disposable glass serological
5.1 Shape—Pipets shall be straight and of one-piece con-
pipets, calibrated “to deliver,” used in measuring volumes of
struction. Any cross section of a pipet taken in a plane
liquids.
perpendicular to the longitudinal axis shall be circular.
2. Referenced Documents
5.2 Delivery Tips—Delivery tips shall be made with a
3
2
gradual taper of 10 to 25 mm for capacities up to 2 cm
2.1 ASTM Standards:
3 3
inclusive, and 15 to 30 mm for the 5-cm and 10-cm size(s).
E438 Specification for Glasses in Laboratory Apparatus
The tip end shall be reasonably perpendicular to the longitu-
E542 Practice for Calibration of Laboratory Volumetric
dinal axis of the pipet and shall be firepolished.
Apparatus
3 3
E920 Specification for Commercially Packaged Laboratory
5.3 Top End—The 10-cm and 25-cm pipet shall have a top
Apparatus
end tooled to a diameter of 7 to 9 mm and shall have an overall
E921 Specification for Export Packaged Laboratory Appa-
length of 15 to 25 mm from the top. The top end shall be
ratus
suitable for plugging with filtering material. All top ends shall
E1133 Practice for Performance Testing of Packaged Labo-
be reasonably perpendicular to the longitudinal axis of the
ratory Apparatus for United States Government Procure-
pipet, and shall be firepolished.
ments
5.4 Dimensions and Outflow Times—The limiting dimen-
3. Terminology
sions and outflow times shall be as shown in Table 1. Outflow
times shall be determined on unplugged pipets, using distilled
3.1 Definitions of Terms Specific to This Standard:
water at 25 6 5°C, and by means of a stopwatch.
3.1.1 accuracy—the expected distribution of mean volumes
around the stated volume.
6. Markings
3.1.2 coeffıcient of variation—the expected distribution of
individual volumes around the mean volume.
6.1 Graduation Markings—Graduation lines shall be be-
tween 0.2 and 0.5 mm in width, and in a plane perpendicular to
3.1.3 disposable pipets—in accordance with this specifica-
the longitudinal axis of the pipet and parallel to each other. A
tion and the expected product performance expressed in this
maingraduationlineshallextendatleastthree-fifthsoftheway
specification, those serological pipets which are to be used one
around the pipet. The values of all main graduation lines shall
time only. Any institution or individual who reuses a dispos-
be in Arabic numerals directly above the line referenced.
able pipet must bear full responsibility for its safety and
Intermediate graduation lines shall extend at least one-fifth of
effectiveness.
the way around the pipet, and least graduation lines shall
4. Materials and Manufacture
extend at least one-seventh of the distance around the pipet.
4.1 The pipets shall be fabricated from borosilicate glass,
The 0 graduation line shall be at least 90 mm below the top of
Type I, ClassAor B; or soda-lime glass, Type II, as defined in
the pipet on all sizes.
Specification E438.
6.2 Identification Markings—Each pipet shall be marked
with the manufacturer’s or vendor’s name or trademark, its
1
This specification is under the jurisdiction of ASTM Committee E41 on
capacity, the symbols “TD” (for “to deliver”) and “20°C,” and
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
Laboratory Ware and Supplies. either a wide band or two narrow bands on the mouthpiece end
CurrenteditionapprovedJune1,2015.PublishedJuly2015.Originallyapproved
to signify that the last drop must be blown out to achieve full
in 1980. Last previous edition approved in 2009 as E714 – 94 (2009). DOI:
delivery.
10.1520/E0714-94R15.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
6.3 Capacity—The pipet capacity, expressed in cubic centi-
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
metres or millilitres, shall be stated on the package. The
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. expected deviation from the stated capacity shall be based on
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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E714−94 (2015)
TABLE 1 Dimensions and Outflow Times
Outside Diameter of Wall
Outflow Time, s
Capacity, Main Graduated 0
Least Value Graduated Portion, Thicknes
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E714 − 94 (Reapproved 2009) E714 − 94 (Reapproved 2015)
Standard Specification for
1
Disposable Glass Serological Pipets
This standard is issued under the fixed designation E714; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This specification covers disposable glass serological pipets, calibrated “to deliver,” used in measuring volumes of liquids.
2. Referenced Documents
2
2.1 ASTM Standards:
E438 Specification for Glasses in Laboratory Apparatus
E542 Practice for Calibration of Laboratory Volumetric Apparatus
E920 Specification for Commercially Packaged Laboratory Apparatus
E921 Specification for Export Packaged Laboratory Apparatus
E1133 Practice for Performance Testing of Packaged Laboratory Apparatus for United States Government Procurements
3. Terminology
3.1 Definitions of Terms Specific to This Standard:
3.1.1 accuracy—the expected distribution of mean volumes around the stated volume.
3.1.2 coeffıcient of variation—the expected distribution of individual volumes around the mean volume.
3.1.3 disposable pipets—in accordance with this specification and the expected product performance expressed in this
specification, those serological pipets which are to be used one time only. Any institution or individual who reuses a disposable
pipet must bear full responsibility for its safety and effectiveness.
4. Materials and Manufacture
4.1 The pipets shall be fabricated from borosilicate glass, Type I, Class A or B; or soda-lime glass, Type II, as defined in
Specification E438.
5. Design
5.1 Shape—Pipets shall be straight and of one-piece construction. Any cross section of a pipet taken in a plane perpendicular
to the longitudinal axis shall be circular.
3
5.2 Delivery Tips—Delivery tips shall be made with a gradual taper of 10 to 25 mm for capacities up to 2 cm inclusive, and
3 3
15 to 30 mm for the 5-cm and 10-cm size(s). The tip end shall be reasonably perpendicular to the longitudinal axis of the pipet
and shall be firepolished.
3 3
5.3 Top End—The 10-cm and 25-cm pipet shall have a top end tooled to a diameter of 7 to 9 mm and shall have an overall
length of 15 to 25 mm from the top. The top end shall be suitable for plugging with filtering material. All top ends shall be
reasonably perpendicular to the longitudinal axis of the pipet, and shall be firepolished.
5.4 Dimensions and Outflow Times—The limiting dimensions and outflow times shall be as shown in Table 1. Outflow times
shall be determined on unplugged pipets, using distilled water at 25 6 5°C, and by means of a stopwatch.
1
This specification is under the jurisdiction of ASTM Committee E41 on Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
ApparatusLaboratory Ware and Supplies.
Current edition approved Nov. 1, 2009June 1, 2015. Published December 2009July 2015. Originally approved in 1980. Last previous edition approved in 20042009 as
E714 – 94 (2004).(2009). DOI: 10.1520/E0714-94R09.10.1520/E0714-94R15.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E714 − 94 (2015)
TABLE 1 Dimensions and Outflow Times
Outside Diameter of Wall
Outflow Time, s
Capacity, Main Graduated 0
Least Value Graduated Portion, Thickness,
3
cm Numbered to At Least
min max
mm min, mm
0.1 0.01 0.01 0.09 3.5 to 4.0 0.5 3 1.0
0.2 0.01 0.02 0.18 3.5 to 4.5 0.5 3 1.0
0.2 0.01 0.05 0.18 3.5 to 4.5 0.5 3 1.0
0.5 0.01 0.1 0.40 4.25 to 4.75 0.5 3 0.8
1.0 0.01 0.1 0.90 4.25 to 4.75 0.5 5 0.8
1.0 0.1 0.1 0.9 4.25 to 4.75 0.5 5 0.8
2.0 0.01 0.1 1.9 5.5 to 6.0 0.5 5 0.8
5.0 0.1 1.0 4.5 7.5 to 8.25 3.0 10 0.8
10.0 0.1 1.0 9.0 9.5 to 11.25 4.5 15 0.8
25.0 0.2 1.0 23.0 15.0 to 16.0 10.0 30 0.85
6. Markings
6.1 Graduation Markings—Graduation lines shall be between 0.2 and 0.5 mm in width, and in a plane perpendicular to the
longitudinal axis of the pipet and parallel to each other. A main graduation line shall extend at least three-fifths of the way around
the pipet. The values of all main graduation l
...