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ASTM E938-05(2011)e1

(Test Method)

Standard Test Method for Effectiveness of Liquid, Gel, or Cream Insecticides Against Adult Human Lice

Standard Test Method for Effectiveness of Liquid, Gel, or Cream Insecticides Against Adult Human Lice

SIGNIFICANCE AND USE
This test method should provide a consistent approach both in terms of test insects and test procedures for the gathering of efficacy data for pediculicides.
Data collection in this manner should be suitable for product development and comparison. In addition, it should be suitable for review by regulatory agencies.
SCOPE
1.1 This test method determines the effectiveness of pediculicidal materials in liquid, gel, or cream form, against the adult human louse, Pediculus humanus humanus Linnaeus, the surrogate subspecies for the human head louse (P.h. capitis). (Only gels or creams that liquefy at 32°C (90°F) can be tested).
1.2 This test method is for the use of those wishing to develop efficacy data, or to compare formulations for head louse control.  
1.3 This test method consists of five replicates for a statistical comparison of formulations.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

General Information

Status
Historical
Publication Date
28-Feb-2011
ICS
65.100.01 - Pesticides and other agrochemicals in general
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Current Stage
Ref Project

Relations

Replaces
ASTM E938-05 - Standard Test Method for Effectiveness of Liquid, Gel, or Cream Insecticides Against Adult Human Lice
Effective Date
01-Mar-2011
Replaced By
ASTM E938/E938M-12 - Standard Test Method for Effectiveness of Liquid, Gel, Cream, or Shampoo Insecticides Against Adult Human Lice (Withdrawn 2017)
Effective Date
01-Apr-2012

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ASTM E938-05(2011)e1 - Standard Test Method for Effectiveness of Liquid, Gel, or Cream Insecticides Against Adult Human LiceASTM E938-05(2011)e1 - Standard Test Method for Effectiveness of Liquid, Gel, or Cream Insecticides Against Adult Human Lice
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ASTM E938-05(2011)e1 - Standard Test Method for Effectiveness of Liquid, Gel, or Cream Insecticides Against Adult Human Lice
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
´1
Designation:E938–05 (Reapproved 2011)
Standard Test Method for
Effectiveness of Liquid, Gel, or Cream Insecticides Against
Adult Human Lice
This standard is issued under the fixed designation E938; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
´ NOTE—Editorial changes were made throughout in April 2011.
1. Scope 3.2 Percent mortality, corrected by Abbott’s Formula, is
determined.
1.1 This test method determines the effectiveness of pedicu-
licidal materials in liquid, gel, or cream form, against the adult
4. Significance and Use
human louse, Pediculus humanus humanus Linnaeus, the
4.1 This test method should provide a consistent approach
surrogate subspecies for the human head louse (P.h. capitis).
both in terms of test insects and test procedures for the
(Onlygelsorcreamsthatliquefyat32°C(90°F)canbetested).
gathering of efficacy data for pediculicides.
1.2 This test method is for the use of those wishing to
4.2 Data collection in this manner should be suitable for
develop efficacy data, or to compare formulations for head
product development and comparison. In addition, it should be
louse control.
suitable for review by regulatory agencies.
1.3 This test method consists of five replicates for a statis-
tical comparison of formulations.
5. Apparatus and Materials
1.4 The values stated in SI units are to be regarded as the
5.1 Test Container—A 9-dram plastic vial, screened at the
standard. The values given in parentheses are for information
bottom with 20-mesh screen, shall be used as the dipping
only.
vessel. A plunger, made from a plastic rod, and a circular
1.5 This standard does not purport to address all of the
screenfitsinsidethevial.Plasticsusedshouldbeaschemically
safety concerns, if any, associated with its use. It is the
unreactive as possible. Plastic vials are to be discarded after
responsibility of the user of this standard to establish appro-
each test.
priate safety and health practices and determine the applica-
5.2 Beakers—A 100-mL beaker is used to contain the
bility of regulatory limitations prior to use.
insecticide into which the test container is dipped. A1000-mL
beaker is used as the container in which the lice are washed
2. Terminology
after treatment.
2.1 Descriptions of Terms Specific to This Standard:
5.3 Heating Surface—A slide dryer, that will provide heat
2.1.1 morbid—unable to move towards heat 1 h after
of approximately 37°C (98°F), is adequate.
treatment: sickly, but not necessarily dying; may recover by 24
5.4 Incubator—The incubator shall be capable of maintain-
h.
ing a temperature of 31.7°C (89°F) and 60 % RH.
2.1.2 moribund—unable to move towards heat (and there-
5.5 Petri dishes, 8.9 cm in diameter and 1.3 cm deep.
fore food) 24 h after treatment; dying.
5.6 Waterbath—Capable of maintaining 32°C (90°F).
3. Summary of Test Method 5.7 Dark Cotton Corduroy,4by4cm.
5.8 Paper Toweling, Stop Watch, Forceps or Camel Hair
3.1 Five replicates of 25 lice each, plus five control repli-
Brush, and Wash Bottle.
cates for each batch of lice, shall be used for each test
concentration or any other variable tested.
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Methods and is the direct
responsibility of Subcommittee E35.12 on Insect Control Agents.
Current edition approved March 1, 2011. Published April 2011. Originally
approved in 1983. Last previous edition approved in 2005 as E938 – 05. DOI: Abbott, W. S., “A Method of Computing the Effectiveness of An Insecticide,”
10.1520/E0938-05R11E01. Journal of Economic Entomology, Vol 18, 1925, pp. 265–267.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
´1
E938–05 (2011)
5.9 Test Insect—The test insect is the human body louse, 7.3 Placethe9-dramvialinthe100-mLpediculicidebeaker,
Pediculus humanus humanus Linnaeus. The present strain was and keep the lice under the pediculicide for a suggested time of
established from the USDA Gainesville strain. It is a suscep- 10 min., (other times may be used, as dictated by the type of
tible strain and, through selection, has adapted to a rabbit host. formulation).
7.4 Remove the test container and blot the bottom of the
5.10 Host Animal—New Zealand white rabbits.
container to remove any remaining liquid.
7.5 Place the 9-dram vial into the 1000-mL beaker contain-
6. Rearing of Test Insects
ing distilled water at 32°C and agitate the container.At the end
6.1 Collect
...

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