ASTM C1338-19

Standard Test Method for Determining Fungi Resistance of Insulation Materials and Facings

SIGNIFICANCE AND USE
2.1 The type of materials used in the manufacture of insulation products and the type of membrane used to face these products can sometimes affect fungi sustenance in the presence of high humidity.  
2.2 This test method is used to determine the relative ability of an insulation and its facing to resist fungal growth under conditions favorable for their development.  
2.3 This test method uses a comparative material to determine the relative ability of a material to resist fungal growth. In some specialized product areas, it is required that no growth take place. In such cases, the use of the comparative material is omitted and the pass/fail criterion is based upon growth.
SCOPE
1.1 This test method covers the determination of the ability of new insulation materials and their facings to resist fungal growth.  
1.2 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Historical
• Publication Date: 28-Feb-2019
• ICS: 91.100.60 - Thermal and sound insulating materials
• Technical Committee: C16 - Thermal Insulation
• Drafting Committee: C16.31 - Chemical and Physical Properties

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: C1338 − 19
Standard Test Method for
Determining Fungi Resistance of Insulation Materials and
1
Facings
This standard is issued under the fixed designation C1338; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Apparatus
3.1 Glassware—Sterile disposable petri dishes, 4 or 6 in.
1.1 This test method covers the determination of the ability
(100 or 150 mm) by 0.6 or 0.75 in. (15 or 20 mm) in size are
of new insulation materials and their facings to resist fungal
preferred. For larger specimens, trays of borosilicate glass or
growth.
baking dishes up to 16 by 20 in. (400 by 600 mm) in size may
1.2 Thevaluesstatedininch-poundunitsaretoberegarded
be used.
as standard. The values given in parentheses are mathematical
3.2 Environmental Chamber or Cabinet—Equipment for
conversions to SI units that are provided for information only
this test method shall maintain a temperature of 82.4 to 86°F
and are not considered standard.
(28 to 30°C) and a relative humidity of 95% (64%).
1.3 This standard does not purport to address all of the
Provisions shall be made to prevent condensation from drip-
safety concerns, if any, associated with its use. It is the
ping on the test specimen.There shall be free circulation of air
responsibility of the user of this standard to establish appro-
around the test chamber.
priate safety, health, and environmental practices and deter-
3.3 Atomizer—A chromatography atomizer capable of pro-
mine the applicability of regulatory limitations prior to use.
2
viding 100000 6 20000 spores/in. (15000 6 3000 spores/
1.4 This international standard was developed in accor-
2
cm ) shall be used for inoculation.
dance with internationally recognized principles on standard-
ization established in the Decision on Principles for the
3.4 Autoclavable Biohazard Bags, or metal pan able to
Development of International Standards, Guides and Recom-
withstand autoclaving.
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee. 4. Reagents and Materials
4.1 Purity of Water—Unless otherwise specified, references
2. Significance and Use
to water shall be understood to mean sterile distilled water or
water of equal purity.
2.1 The type of materials used in the manufacture of
insulation products and the type of membrane used to face
4.2 Inoculum:
these products can sometimes affect fungi sustenance in the
2
Fungi ATCC
presence of high humidity.
Aspergillus niger 9642
Aspergillus versicolor 11 730
2.2 Thistestmethodisusedtodeterminetherelativeability
Penicillium funiculosum 11 797
Chaetomium globosum 6205
of an insulation and its facing to resist fungal growth under
Aspergillus flavus 9643
conditions favorable for their development.
4.3 Cultures—Maintain cultures of the Aspergillus fungi
2.3 This test method uses a comparative material to deter-
separately on Czapek Dox agar (see Note 1). Culture the
minetherelativeabilityofamaterialtoresistfungalgrowth.In
Chaetomiumglobosumonstripsofcellulosefilterpaperonthe
some specialized product areas, it is required that no growth
surfaceofCzapekDoxagar.MaintainthePenicilliumfungion
take place. In such cases, the use of the comparative material
Sabouraud Dextrose agar. The stock cultures may be kept for
is omitted and the pass/fail criterion is based upon growth.
not more than 4 months at 43 6 7°F (6 6 4°C) at which time
subcultures shall be made, and new stocks selected from the
1
ThistestmethodisunderthejurisdictionofASTMCommitteeC16onThermal
2
InsulationandisthedirectresponsibilityofSubcommitteeC16.31onChemicaland The sole source of supply of the cultures known to the committee at this time
Physical Properties. is American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville,
Current edition approved March 1, 2019. Published March 2019. Originally MD20852.Ifyouareawareofalternativesuppliers,pleaseprovidethisinformation
approved in 1996. Last previous edition approved in 2014 as C1338–14. DOI: to ASTM International Headquarters. Your comments will receive careful consid-
1
10.1520/C1338-19. erationatameetingoftheresponsibletechnicalcommittee, whichyoumayattend.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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C1338 − 19
subcultures. If genetic or physiological changes occur, obtain throughatleasta0.24-in.(6-mm)layerofglasswoolcontained
new cultures. Incubate subcultures used for preparing new inaglassfunnel,intoasterileflask.Thisprocessisintendedto
stock cultures or the spore suspe
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: C1338 − 14 C1338 − 19
Standard Test Method for
Determining Fungi Resistance of Insulation Materials and
1
Facings
This standard is issued under the fixed designation C1338; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method covers the determination of the ability of new insulation materials and their facings to resist fungal growth.
1.2 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical
conversions to SI units that are provided for information only and are not considered standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Significance and Use
2.1 The type of materials used in the manufacture of insulation products and the type of membrane used to face these products
can sometimes affect fungi sustenance in the presence of high humidity.
2.2 This test method is used to determine the relative ability of an insulation and its facing to resist fungal growth under
conditions favorable for their development.
2.3 This test method uses a comparative material to determine the relative ability of a material to resist fungal growth. In some
specialized product areas, it is required that no growth take place. In such cases, the use of the comparative material is omitted
and the pass/fail criterion is based upon growth.
3. Apparatus
3.1 Glassware—Sterile disposable petri dishes, 4 or 6 in. (100 or 150 mm) by 0.6 or 0.75 in. (15 or 20 mm) in size are preferred.
For larger specimens, trays of borosilicate glass or baking dishes up to 16 by 20 in. (400 by 600 mm) in size may be used.
3.2 Environmental Chamber or Cabinet—Equipment for this test method shall maintain a temperature of 82.4 to 86°F (28 to
30°C) and a relative humidity of 95 % (64 %). Provisions shall be made to prevent condensation from dripping on the test
specimen. There shall be free circulation of air around the test chamber.
2
3.3 Atomizer—A chromatography atomizer capable of providing 100 000 6 20 000 sporesspores/in.⁄in. (15 000 6 3000
2
spores/cm ) shall be used for inoculation.
3.4 Autoclavable Biohazard Bags, or metal pan able to withstand autoclaving.
4. Reagents and Materials
4.1 Purity of Water—Unless otherwise specified, references to water shall be understood to mean sterile distilled water or water
of equal purity.
4.2 Inoculum:
1
This test method is under the jurisdiction of ASTM Committee C16 on Thermal Insulation and is the direct responsibility of Subcommittee C16.31 on Chemical and
Physical Properties.
Current edition approved Jan. 1, 2014March 1, 2019. Published February 2014March 2019. Originally approved in 1996. Last previous edition approved in 20082014 as
C1338C1338 – 14.–08. DOI: 10.1520/C1338-14.10.1520/C1338-19.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
C1338 − 19
2
Fungi ATCC
Aspergillus niger 9642
Aspergillus versicolor 11 730
Penicillium funiculosum 11 797
Chaetomium globosum 6205
Aspergillus flavus 9643
4.3 Cultures—Maintain cultures of the Aspergillus fungi separately on Czapek Dox agar (see Note 1). Culture the Chaetomium
globosum on strips of cellulose filter paper on the surface of Czapek Dox agar. Maintain the Penicillium fungi on Sabouraud
Dextrose agar. The stock cultures may be kept for not more than 4 months at 43 6 7°F (6 6 4°C) at which time subcultures shall
be made, and new stocks selected from the subcultures. If genetic or physiological changes occur, obtain new cultures. Incubate
subcultures used for preparing new stock cultures or the spore suspension at 86 6 4°F (30 6 2°C) for 5 days or longer.
NOTE 1—This
...