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ASTM D7788-14

(Practice)

Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology

Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology

SIGNIFICANCE AND USE
4.1 This practice is intended for the collection of airborne fungal spores or fragments, or both, using inertial impaction.  
4.2 It is the responsibility of the user to assure that they are in compliance with all local, state and federal regulations governing the inspection of buildings for fungal colonization and the collection of associated samples.  
4.3 This practice is intended to provide the user with a basic understanding of the equipment, materials and instructions necessary to effectively collect air samples using an inertial impactor.  
4.4 This practice, when properly executed, may also be used for the evaluation of other types of airborne particles with the capturing characteristics appropriate for inertial impactor, and for which appropriate analytical methods exist. Such particles may include dust mites, skin cells, pollen, and other materials.
SCOPE
1.1 The purpose of this practice is to describe procedures for the collection of airborne fungal spores or fragments, or both, using inertial impaction sampling techniques.  
1.2 This practice is not intended to limit the user from the collection of other airborne particulates that may be of interest and captured through this technique.  
1.3 This practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis.  
1.4 This practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these issues.  
1.5 This practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the user's responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
31-May-2014
ICS
13.040.20 - Ambient atmospheres
Technical Committee
D22 - Air Quality
Drafting Committee
D22.08 - Assessment, Sampling, and Analysis of Microorganisms
Current Stage
Ref Project

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ASTM D7788-14 - Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction MethodologyASTM D7788-14 - Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology
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ASTM D7788-14 - Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D7788 − 14
Standard Practice for
Collection of Total Airborne Fungal Structures via Inertial
1
Impaction Methodology
This standard is issued under the fixed designation D7788; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2
2.1 ASTM Standards:
1.1 The purpose of this practice is to describe procedures for
D1356 Terminology Relating to Sampling and Analysis of
the collection of airborne fungal spores or fragments, or both,
Atmospheres
using inertial impaction sampling techniques.
D3195/D3195M Practice for Rotameter Calibration
1.2 This practice is not intended to limit the user from the
D4840 Guide for Sample Chain-of-Custody Procedures
collection of other airborne particulates that may be of interest
D6044 Guide for Representative Sampling for Management
and captured through this technique.
of Waste and Contaminated Media
D7391 Test Method for Categorization and Quantification of
1.3 This practice presumes that the user has a fundamental
Airborne Fungal Structures in an Inertial Impaction
understanding of field investigative techniques related to the
Sample by Optical Microscopy
scientific process, and sampling plan development and imple-
mentation. It is important to establish the related hypothesis to
3. Terminology
be tested and the supporting analytical methodology needed in
3.1 Definitions—For definitions and terms not listed here,
order to identify the sampling media to be used and the
see Terminology D1356.
laboratory conditions for analysis.
3.1.1 inertial impactor, n—a device designed for the impac-
tion of particles that are separated from the air stream by inertia
1.4 This practice does not address the development of a
onto a collection surface. D7391
formal hypothesis or the establishment of appropriate and
3.1.1.1 Discussion—Inertial impactors are available in
defensible investigation and sampling objectives. It is pre-
many designs including “slit” and “circular” jets.
sumed the investigator has the experience and knowledge base
3.1.1.2 Discussion—Allows for the identification to genus
to address these issues.
3
or group of fungi detected, quantification to spores/m , and
1.5 This practice does not provide the user sufficient infor-
general assessment of background debris. Identification of
mation to allow for interpretation of the analytical results from
pollen, hyphal fragments and other airborne particulate may be
sample collection. It is the user’s responsibility to seek or
included.
obtain the information and knowledge necessary to interpret
3.1.2 sample, n—a portion of a population. A portion of
the sample results reported by the laboratory.
material that is taken for testing or record purposes. D6044
1.6 The values stated in SI units are to be regarded as
3.1.3 sample, representative, n—a sample collected in such
standard. No other units of measurement are included in this
a manner that it reflects one or more characteristics of interest
standard.
(as defined by the project objectives) of a population from
which it is collected. D6044
1.7 This standard does not purport to address all of the
3.1.3.1 Discussion—Populations of airborne fungal spores
safety concerns, if any, associated with its use. It is the
are typically not homogeneous.
responsibility of the user of this standard to establish appro-
3.2 Definitions of Terms Specific to This Standard:
priate safety and health practices and determine the applica-
3.2.1 aerodynamic diameter (d ), n—the diameter of a unit
bility of regulatory limitations prior to use. a
density sphere having the same inertial properties as the
particle under analysis under the same conditions.
1
This practice is under the jurisdiction of ASTM Committee D22 on Air Quality
2
and is the direct responsibility of Subcommittee D22.08 on Sampling and Analysis For referenced ASTM standards, visit the ASTM website, www.astm.org, or
of Mold. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved June 1, 2014. Published July 2014. DOI: 10.1520/ Standards volume information, refer to the standard’s Document Summary page on
D7788-14. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7788 − 14
3.2.1.1 Discussion—For fungal spores this is generally 5. Preparation of Sampling Equipment
based on a water droplet, (spherical particle) having a density
5.1 Equipment List:
3
of 1 g/cm . Aerodynamic diameter has been developed to
5.1.1
...

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5.1 Air permits from regulatory agencies often require measurements of opacity from stationary air pollution point sources in the outdoor ambient environment. Opacity has been visually measured by certified smoke readers in accordance with USEPA (USEPA Method 9). DCOT is also a method to determine plume opacity in the outdoor ambient environment.  
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1.3 Procedures for random sampling of units within dwellings having multiple units are not included.  
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1.5 The values stated in SI units are to be regarded as the standard.  
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1.2 This practice does not apply to sensors or instruments that remotely measure atmospheric pollutants using open path, lidar, or imaging technology.  
1.3 The evaluation procedures contained in this practice are for sensors that alone or in combination measure outdoor criteria pollutants in ambient air: particulate matter (PM2.5 and PM10), sulfur dioxide (SO2), ozone (O3), carbon monoxide (CO), or nitrogen dioxide (NO2) at concentrations that are relevant to public health.  
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3.2 IEEE/ASTM SI-10 does not list all the conversion factors commonly used in air pollution and meteorological fields. This practice supplements IEEE/ASTM SI-10.  
3.3 The values reported here were obtained from a number of standard publications. They were adjusted to five figures and organized in a rational order. All values reflect the latest information from the 16th General Conference on Weights and Measurements held in 1979.  
3.4 The factors in Table 1 are provided to change units of measurement from one system to related units in other systems, as well as to smaller or larger units in the same system.  
3.5 Values of units in the left column may be converted to values of units in the right column merely by multiplying by the conversion factor provided in the center column.  (A) For specific applications and exceptions, see Terminology D1356.
SCOPE
1.1 This practice provides units and factors useful for members of the air pollution and meteorological communities.  
1.2 This practice is used together with IEEE/ASTM SI-10, which discusses SI units and contains selected conversion factors for inter-relation of SI units and some commonly used non-metric units.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM
ASTM D2914-15(2022)(Test Method)
Standard Test Methods for Sulfur Dioxide Content of the Atmosphere (West-Gaeke Method)

SIGNIFICANCE AND USE
5.1 Sulfur dioxide is a major air pollutant, commonly formed by the combustion of sulfur-bearing fuels. The Environmental Protection Agency (EPA) has set primary and secondary air quality standards (7) that are designed to protect the public health and welfare.  
5.2 The Occupational Safety and Health Administration (OSHA) has promulgated exposure limits for sulfur dioxide in workplace atmospheres (8).  
5.3 These methods have been found satisfactory for measuring sulfur dioxide in ambient and workplace atmospheres over the ranges pertinent in 5.1 and 5.2.  
5.4 Method A has been designed to correspond to the EPA-Designated Reference Method (7) for the determination of sulfur dioxide.
SCOPE
1.1 These test methods cover the bubbler collection and colorimetric determination of sulfur dioxide (SO2) in the ambient or workplace atmosphere.  
1.2 These test methods are applicable for determining SO2 over the range from approximately 25 μg/m3 (0.01 ppm(v)) to 1000 μg/m3 (0.4 ppm(v)), corresponding to a solution concentration of 0.03 μg SO2/mL to 1.3 μg SO2/mL. Beer's law is followed through the working analytical range from 0.02 μg SO2/mL to 1.4 μg SO2/mL.  
1.3 The lower limit of detection is 0.075 μg SO2/mL (1),2 representing an air concentration of 25 μg SO2/m3 (0.01 ppm(v)) in a 30-min sample, or 13 μg SO2/m3 (0.005 ppm(v)) in a 24-h sample.  
1.4 These test methods incorporate sampling for periods between 30 min and 24 h.  
1.5 These test methods describe the determination of the collected (impinged) samples. A Method A and a Method B are described.  
1.6 Method A is preferred over Method B, as it gives the higher sensitivity, but it has a higher blank. Manual Method B is pH-dependent, but is more suitable with spectrometers having a spectral band width greater than 20 nm.
Note 1: These test methods are applicable at concentrations below 25 μg/m3 by sampling larger volumes of air if the absorption efficiency of the particular system is first determined, as described in Annex A4.
Note 2: Concentrations higher than 1000 μg/m3 can be determined by using smaller gas volumes, larger collection volumes, or by suitable dilution of the collected sample with absorbing solution prior to analysis.  
1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.8 Warning—Mercury has been designated by many regulatory agencies as a hazardous material that can cause serious medical issues. Mercury, or its vapor, has been demonstrated to be hazardous to health and corrosive to materials. Caution should be taken when handling mercury and mercury containing products. See the applicable product Safety Data Sheet (SDS) for additional information. Users should be aware that selling mercury and/or mercury containing products into your state or country may be prohibited by law.  
1.9 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific precautionary statements, see 8.3.1, Section 9, and A3.1.3.  
1.10 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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