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ASTM D5806-95(2003)

(Test Method)

Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration

Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration

SIGNIFICANCE AND USE
This test method is used to determine the percent actives in each type of the disinfectant quaternary ammonium salts, and also in the disinfectant products. Quaternary ammonium compounds being the active ingredients in disinfectant-type products require accurate determination to assess the cost and antimicrobial performance of such products.
SCOPE
1.1 This test method covers a potentiometric titration procedure for determining active matter in disinfectant quaternary ammonium salts. This test method is intended for the analysis of quaternary ammonium salts used as disinfectants, and only applies to the following commonly used quaternary ammonium salts: n-alkyldimethylbenzylammonium chloride (see Fig. 1), cetyltrimethylammonium chloride, and a blend of n-octyldecyl dimethylammonium chloride, di-n-octyl dimethylammonium chloride, and di-n-decyldimethyl ammonium chloride (see Fig. 2). Also, this test method can be applied to the analysis of disinfectant type products where the formula ingredients are known and the quaternary ammonium salt is one of the above. Interferences such as amines oxides and betaines present in disinfectant formulations were not tested.
1.2 This disinfectant quaternary ammonium salt conforms to the structures in Fig. 1 and Fig. 2.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautionary information, see Section 8.

General Information

Status
Historical
Publication Date
09-Oct-1995
ICS
71.100.35 - Chemicals for industrial and domestic disinfection purposes
Technical Committee
D12 - Soaps and Other Detergents
Drafting Committee
D12.12 - Analysis and Specifications of Soaps, Synthetics, Detergents and their Components
Current Stage
Ref Project

Relations

Replaces
ASTM D5806-95 - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration
Effective Date
10-Oct-1995
Replaced By
ASTM D5806-95(2009) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration
Effective Date
01-Oct-2009

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ASTM D5806-95(2003) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric TitrationASTM D5806-95(2003) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration
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ASTM D5806-95(2003) - Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information.
Designation:D5806–95(Reapproved 2003)
Standard Test Method for
Disinfectant Quaternary Ammonium Salts by Potentiometric
1
Titration
This standard is issued under the fixed designation D5806; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope Methods for Analysis and Testing of Industrial and Spe-
3
cialty Chemicals
1.1 This test method covers a potentiometric titration pro-
cedure for determining active matter in disinfectant quaternary
3. Terminology
ammonium salts. This test method is intended for the analysis
3.1 Definitions—See Terminology D459.
of quaternary ammonium salts used as disinfectants, and only
appliestothefollowingcommonlyusedquaternaryammonium
4. Summary of Test Method
salts: n-alkyldimethylbenzylammonium chloride (see Fig. 1),
4.1 Disinfectant type quaternary ammonium compounds
cetyltrimethylammoniumchloride,andablendof n-octyldecyl
present,astheactivematerialsindisinfectanttypeproductsare
dimethylammonium chloride, di-n-octyl dimethylammonium
titrated potentiometrically in an aqueous medium with a
chloride,anddi-n-decyldimethylammoniumchloride(seeFig.
standard solution of sodium lauryl sulphate using a nitrate
2). Also, this test method can be applied to the analysis of
ion-selective electrode or a surfactant electrode (see also Test
disinfectant type products where the formula ingredients are
Method D1681). In this potentiometric titration, the reaction
known and the quaternary ammonium salt is one of the above.
involves the formation of a complex between the disinfectant
Interferences such as amines oxides and betaines present in
quaternary ammonium compound and the anionic surfactant
disinfectant formulations were not tested.
which then precipitates. At the end point, the nitrate ion
1.2 This disinfectant quaternary ammonium salt conforms
electrode or surfactant electrode appears to respond to an
to the structures in Fig. 1 and Fig. 2.
excess of titrant with a potential change large enough to give a
1.3 This standard does not purport to address all of the
well defined inflection in the titration curve.
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro-
5. Significance and Use
priate safety and health practices and determine the applica-
5.1 Thistestmethodisusedtodeterminethepercentactives
bility of regulatory limitations prior to use. For specific
in each type of the disinfectant quaternary ammonium salts,
precautionary information, see Section 8.
and also in the disinfectant products. Quaternary ammonium
compounds being the active ingredients in disinfectant-type
2. Referenced Documents
products require accurate determination to assess the cost and
2
2.1 ASTM Standards:
antimicrobial performance of such products.
D459 Terminology Relating to Soaps and Other Detergents
D1193 Specification for Reagent Water
6. Apparatus
D1681 TestMethodforSyntheticAnionicActiveIngredient
4
6.1 Autotitration System, with 10-mL buret capacity, or
in Detergents by Cationic Titration Procedure
5 6
20-mL buret capacity, magnetic stirrer, evaluating ruler,
D3049 Test Method for Synthetic Anionic Ingredient by
7
titroprocessor with 10-mLburet capacity or equivalent autoti-
Cationic Titration
tration system.
E180 Practice for Determining the Precision of ASTM
3
Withdrawn. The last approved version of this historical standard is referenced
1
This test method is under the jurisdiction ofASTM Committee D12 on Soaps on www.astm.org.
4
and Other Detergents and is the direct responsibility of Subcommittee D12.12 on Metrohm-Brinkmann E-536, or equivalent, has been found satisfactory.Avail-
Analysis of Soaps and Synthetic Detergents. able from Brinkmann Instruments Inc., Cantiague Rd., Westbury, NY 11590.
5
Current edition approved Oct. 10, 1995. Published December 1995. DOI: Potentiograph/E-535 and Dosimat/E-459, or equivalent, have been found
10.1520/D5806-95R03. satisfactory. Available from Brinkmann Instruments Inc.
2 6
For referenced ASTM standards, visit the ASTM website, www.astm.org, or Evaluating Ruler EA-893, or equivalent, has been found satisfactory.Available
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM from Brinkmann Instruments Inc.
7
Standards volume information, refer to the standard’s Document Summary page on Metrohm-BrinkmannTitroprocessor670hasbeenfoundsatisfactory.Available
the ASTM website. from Brinkmann Instruments Inc.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1

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Standard Test Method for Disinfectant Quaternary Ammonium Salts by Potentiometric Titration

SIGNIFICANCE AND USE
5.1 This test method is used to determine the percent actives in each type of the disinfectant quaternary ammonium salts, and also in the disinfectant products. Quaternary ammonium compounds being the active ingredients in disinfectant-type products require accurate determination to assess the cost and antimicrobial performance of such products.
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1.1 This test method covers a potentiometric titration procedure for determining active matter in disinfectant quaternary ammonium salts. This test method is intended for the analysis of quaternary ammonium salts used as disinfectants, and only applies to the following commonly used quaternary ammonium salts: n-alkyldimethylbenzylammonium chloride (see Fig. 1), cetyltrimethylammonium chloride, and a blend of n-octyldecyl dimethylammonium chloride, di-n-octyl dimethylammonium chloride, and di-n-decyldimethyl ammonium chloride (see Fig. 2). Also, this test method can be applied to the analysis of disinfectant type products where the formula ingredients are known and the quaternary ammonium salt is one of the above. Interferences such as amines oxides and betaines present in disinfectant formulations were not tested.  X−  =  chloride, and    R  =  aliphatic, normal C8 − C22.    
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5.1 This practice is to be used for the removal of virucidal agents from test product-virus mixtures, or from test product-neutralizer-virus mixtures, at or after the contact period and before the inoculation of these mixtures into host systems for assay of viral infectivity.  
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5.4 This practice is compatible with organic soil loads, hard water, disinfectants containing organic solvents, and chemical neutralizers.
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1.1 This practice is intended to be used to reduce the cytotoxic level of the virus-test product mixture prior to assaying for viral infectivity. It is used in conjunction with evaluations of the virucidal efficacy of disinfectant solutions, wipes, trigger sprays, or pressurized disinfectant spray products intended for use on inanimate, nonporous environmental surfaces. This practice may also be used in the evaluation of hygienic handwashes/handrubs, or for other special applications. The practice may be employed with all viruses and host systems.
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1.3 This practice utilizes gel filtration technology. The effectiveness of the practice is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns as well as the conditions of centrifugation. The effectiveness of this practice is maximized by investigator practice and experience with gel filtration techniques.  
1.4 This practice will aid in the reduction, but not necessarily elimination, of test product toxicity while preserving the titer of the input virus.  
1.5 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
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Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection

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5.1 This practice may be used to determine if a pre-saturated or impregnated towelette demonstrates antimicrobial effectiveness as a disinfectant on hard surfaces. This practice provides survivor results in the form of a qualitative endpoint (growth positive versus growth negative). The results generated by following this practice do not provide for specific quantitative reductions.
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1.1 This practice is designed to evaluate the antimicrobial activity of pre-saturated or impregnated towelettes when used as a hard surface disinfectant.  
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5.1 The effectiveness of antimicrobial agents incorporated into disinfectants, sanitizers, and antiseptics is measured by their ability to kill microorganisms within a specified contact time. Hence, accurate determination of antimicrobial effectiveness requires complete and immediate inactivation (neutralization) of the antimicrobial agent. Inefficient or incomplete neutralization will permit killing or inactivation of microorganisms to continue beyond the experimental exposure time, resulting in an overestimation of antimicrobial activity.  
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1.2 Knowledge of microbiological and statistical techniques is required for these procedures.
Note 1: These methods are not suitable when testing the virucidal activity of microbicides (see Test Method E1482).  
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Standard Test Method for Determining the Bacteria-Eliminating Effectiveness of Healthcare Personnel Hand Rub Formulations Using Hands of Adults

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5.1 Hand hygiene is considered one of the most important measures for preventing the spread of infectious microorganisms. Hand rubs reduce the microbial load on the hands without the use of soap and water, and are thus an important tool in the practice of good hand hygiene. Alcohol-based hand rubs are recommended in healthcare settings for use on hands that are not visibly soiled. They are formulated to be applied full strength to dry hands, “rubbed in” until dry, and are not rinsed off.  
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5.2.1 In this method, application to the hands of a small volume of high-titer test bacteria suspension minimizes soil load such that the skin is completely dry prior to application of the test material. Further, by applying the bacterial suspension only prior to those test material application cycles followed by sampling, excessive buildup of killed bacteria on the hands is avoided, and the potential impact of non-volatile test product ingredients on bacteria-eliminating effectiveness after ten consecutive applications can be specifically assessed.  
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5.1 The guide may be used to demonstrate the effectiveness of topical antimicrobial products using pigskin as a surrogate for human skin and the cup scrub technique for sampling.  
5.2 The techniques described can be used to simulate Test Method E1174 and will use the pigskin substrate to overcome limitations posed by exposure of human subjects to potentially pathogenic microorganisms, while offering the benefit of applicability to a wide variety of hand-washing conditions that cannot be simulated in test tubes.  
5.3 Use of the pigskin surrogate offers less expensive and higher throughput screening.
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1.1 This guide is designed to demonstrate the effectiveness of hand hygiene topical antimicrobial products using pigskin as a surrogate model.  
1.2 Knowledge of microbiological techniques is required for these procedures.  
1.3 This standard guide can be used to evaluate topical antimicrobial handwash or handrub formulations.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
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Standard Test Method for Determining Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor Using the Single Tube Method

SIGNIFICANCE AND USE
5.1 The Single Tube Method is designed to evaluate the efficacy of disinfectants against biofilm grown in the CDC biofilm reactor following the procedures outlined in Practice E3161. Biofilm grown in the CDC reactor is representative of biofilm that forms under high fluid shear on surfaces conducive to biofilm formation.  
5.1.1 Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. Biofilm growth reactors are engineered to produce biofilm with specific characteristics (2). Altering either the engineered system or operating conditions will modify those characteristics as well as the physicochemical environment. The goal in biofilm research and testing is to choose the growth reactor and operating conditions that generate the most relevant biofilm for the particular study.  
5.2 The test method was designed to determine the log10 reduction in bacteria after exposure to a disinfectant in a closed system.  
5.3 The test method uses 50 mL conical tubes. The conical geometry allows for disinfectant exposure to biofilm on all surfaces of the coupon. For foaming disinfectants or for disinfectants requiring a larger volume of neutralizer, 250 mL conical tubes are used which preserve the required geometry and allow for greater neutralization capacity.  
5.4 Each test includes three untreated control coupons (exposed to buffered dilution water) and five treated coupons (per disinfectant/concentration/contact time combination).
SCOPE
1.1 This test method specifies the operational parameters required to perform a quantitative liquid disinfectant efficacy test against bacterial biofilm.  
1.2 The test method was optimized and validated for a Pseudomonas aeruginosa or Staphylococcus aureus biofilm grown in the CDC Biofilm Reactor (E3161). The method is suitable for evaluating additional bacteria grown using the procedures outlined in methods with comparable coupon dimensions such as Practice E3161, Test Method E2562, or Test Method E2196.  
1.3 Disinfectant preparation and contact time are used in the assessment according to the manufacturer’s instructions for use.  
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1.5 This test method describes a harvesting and analysis procedure which includes vortexing and sonicating treated and untreated control biofilm, and recovery of culturable cells using filtration to lower the limit of detection. Biofilm population density is recorded as log10 colony-forming units per coupon. Efficacy is reported as a log10 reduction of culturable cells.  
1.6 Basic microbiology training is required to perform this assay.  
1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
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ASTM E2946-21(Test Method)
Standard Test Method for Determining the Bacteria-Reducing Effectiveness of Food-Handler Handwash Formulations Using Hands of Adults

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5.1 Hand hygiene is considered one of the most important measures for preventing the spread of infectious microorganisms and is critical for reducing the incidence of food-borne disease. Food-handling settings are unique in that moderate to heavy soil load present on hands often can influence the ability of a product to remove or kill microorganisms (3, 4). Test methods are needed for assessing the efficacy of hand hygiene products under conditions representative of those encountered in a food-handling environment.  
5.2 This test method is specifically designed to evaluate the effectiveness of food-handler products to kill and remove bacteria from experimentally-contaminated hands under conditions of moderate to heavy organic soil load. The inclusion of soils typical of food service setting makes this a methodology more appropriate than Test Method E1174, which was designed to evaluate healthcare personnel hand washes and does not include an option to include soil (4).
SCOPE
1.1 This test method is designed to determine the activity of food-handler handwashes against transient bacterial flora on the hands.  
1.2 Performance of this procedure requires the knowledge of regulations pertaining to the protection of human subjects (1)2.  
1.3 This test method should be performed by persons with training in microbiology, in facilities designed and equipped for work with potentially infectious agents at biosafety level 2 (2).  
1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For more specific precautionary statements see 8.1.1.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Standard Test Method for Evaluation of Hygienic Handwash and Handrub Formulations for Virus-Eliminating Activity Using the Entire Hand

SIGNIFICANCE AND USE
5.1 This test method is designed to evaluate the virus-eliminating activity of hygienic handwash and handrub agents from experimentally-contaminated hands. Such formulations may be further assessed in a clinical trial for their effectiveness in the field. This test method incorporates whole-hand exposure and reflects actual use conditions such as friction during hand decontamination, and enables alternative product forms such as alcohol- or non-alcohol-based liquids, gels, and foams to be tested according to label directions. It is meant to extend, if required, the results of testing with Test Method E1838, which gives precise reductions in viral infectivity on a limited area of the hands. It may also serve as an alternative test method when product form is not amenable to testing by Test Method E1838.  
5.2 This test method is not meant for use with surgical hand scrubs or preoperative skin preparations.
Note 2: Application of viruses on the entire surface of both hands entails a greater risk to the subjects than using fingerpads only. Therefore, greater care is needed to ensure that the hands of the participants are free from any apparent damage. Also, virus preparations must be thoroughly screened for, or documented to be free from, extraneous or adventitious pathogens before use in such tests.
SCOPE
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1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. The user should consult a reference for laboratory safety recommendations. (3-5)  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

  • Standard
    7 pages
    English language
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  • Standard
    7 pages
    English language
    sale 15% off