ASTM D4200-82(2011)
(Test Method)Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters
Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters
SIGNIFICANCE AND USE
This test method may be applied to determine the suitability of grid-marked membrane filters for use in bacteriological culture techniques for the detection and enumeration of bacterial organisms.
A particularly sensitive organism and growth conditions have been selected for this test method in order to maximize sensitivity to toxic materials possibly present in the inks used for grid-marking membrane filters.
SCOPE
1.1 This test method describes a procedure whereby the user of ink-gridded membrane filters in water quality studies can ascertain whether or not the grid lines are toxic and inhibitory to bacterial growth when the membrane and its entrapped bacteria are incubated on a suitable media.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
General Information
Relations
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D4200 − 82 (Reapproved 2011)
Standard Test Method for
Evaluating Inhibitory Effects of Ink Grids on Membrane
Filters
This standard is issued under the fixed designation D4200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5. Significance and Use
1.1 Thistestmethoddescribesaprocedurewherebytheuser 5.1 This test method may be applied to determine the
of ink-gridded membrane filters in water quality studies can suitability of grid-marked membrane filters for use in bacte-
ascertain whether or not the grid lines are toxic and inhibitory riological culture techniques for the detection and enumeration
to bacterial growth when the membrane and its entrapped of bacterial organisms.
bacteria are incubated on a suitable media.
5.2 Aparticularly sensitive organism and growth conditions
1.2 The values stated in SI units are to be regarded as have been selected for this test method in order to maximize
standard. No other units of measurement are included in this sensitivity to toxic materials possibly present in the inks used
standard. for grid-marking membrane filters.
1.3 This standard does not purport to address all of the
6. Apparatus
safety concerns, if any, associated with its use. It is the
6.1 Incubator, capable of maintaining temperatures of
responsibility of the user of this standard to establish appro-
44.56 0.2°C.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
6.2 Membrane Filtration Units.
6.3 Vacuum Source with trap vessel.
2. Referenced Documents
6.4 Forceps, blunt-nosed.
2.1 ASTM Standards:
D1129 Terminology Relating to Water
6.5 Autoclave or other sterilizing equipment.
D1193 Specification for Reagent Water
6.6 Expendables:
6.6.1 Gridded membrane filters.
3. Terminology
6.6.2 1-mL and 10-mL pipets.
3.1 Definitions—For definitions of terms used in this test
6.6.3 Petri dishes (50-mm) containing 6 to 8 mL of agar
method, refer to Terminology D1129.
medium or a 100-mm dish with 20 6 2 mLof agar medium, or
both.
4. Summary of Test Method
6.6.4 Erlenmeyer flasks.
4.1 A heavy bacterial suspension is filtered through a
gridded membrane filter.The bacterial concentration employed
7. Reagents and Materials
is sufficient to cover much of the membrane with bacterial
7.1 Purity of Water—Unless otherwise indicated, reference
colonies.
to water shall be understood to mean reagent water conforming
4.2 After filtration the membrane is incubated on a suitable to reagent water Type II of Specification D1193.
medium and the distribution of the colonies and shape of the
7.2 M-FC Agar with Rosolic Acid or equivalent (henceforth
colonies noted in the area around each grid line.
referred to as agar medium), formulated, prepared, and dis-
pensed in accordance with the manufacturer’s specifications.
This test method is under the jurisdiction of ASTM Committee D19 on Water
7.3 Tryptone Soya Broth or equivalent (henceforth referred
and is the direct responsibility of Subcommittee D19.08 on Membranes and Ion
to as broth medium), formulated, prepared, and dispensed in
Exchange Materials.
Current edition approved May 1, 2011. Published June 2011. Originally accordance with the manufacturer’s specifications.
approved in 1982. Last previous edition approved in 2003 as D4200 – 82 (2003).
7.4 Peptone Water, 0.1 %, ster
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.