ASTM E555-95

Practice for Determining Acute Oral LD50 for Testing Vertebrate Control Agents (Withdrawn 2000)

SCOPE
1.1 This practice covers the determination of acute oral LD50 for testing vertebrate control agents. Because the acute oral LD50 functions as a significant criterion for evaluation of vertebrate control agents, general guidelines for performing the tests have been stated. However, since specific modifications may be required for each biological group, this practice represents only a common denominator. Necessary modifications and additions will be cited in the appropriate test documents for each biological group.

General Information

• Status: Withdrawn
• Publication Date: 09-Nov-1995
• Withdrawal Date: 09-Oct-2000
• ICS: 71.100.99 - Other products of the chemical industry
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or discontinued.
Contact ASTM International (www.astm.org) for the latest information.
Designation: E 555 – 95
Standard Practice for
Determining Acute Oral LD50 for Testing Vertebrate Control
Agents
This standard is issued under the fixed designation E 555; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Vertebrate animal control as a science is very primitive, lacking many of the research tools and
procedures which are well established in other biological areas. Indeed the field still remains more of
an art or skill highly dependent upon field experience usually garnered by trial and error. This is
particularly true in dealing with the higher forms that are neither domestic nor commensal. All are
capable of elementary reason as well as learned behavioral patterns; thus pesticidal work with these
forms has been very difficult and in some areas impossible to standardize. However, the committee
recognizes that the effort to do so must be made both to improve the science related and to provide
some reasonable safeguards for legitimate environmental concerns.
1. Scope used (Bliss, 1938 (4); and Finney, 1971 (5)).
1.1 This practice covers the determination of acute oral
4. Dosage levels
LD50 for testing vertebrate control agents. Because the acute
4.1 Graduated dosages in mg of chemical per kg of body
oral LD50 functions as a significant criterion for evaluation of
weight are used. The starting point should be selected arbi-
vertebrate control agents, general guidelines for performing the
trarily by the investigator based on the available toxicological
tests have been stated. However, since specific modifications
information concerning the chemical.
may be required for each biological group, this practice
4.1.1 A constant geometric factor between doses is not
represents only a common denominator. Necessary modifica-
necessary with procedure (a) (1). Bracketing the suspected
tions and additions will be cited in the appropriate test
LD50 is desirable so that all-effect and no-effect levels are
documents for each biological group.
included.
2. Referenced Documents 4.1.2 A constant geometric progression between doses must
be used for procedure (b) (2,3).
2.1 ASTM Standards:
E 758 Test Method for Mammalian Acute Percutaneous
5. Carrier
Toxicity
5.1 Distilled water should be the carrier of choice. Agents
E 1055 Test Method for Evaluation of Eye Irritation in
2 insoluble in water should either be suspended in a 0.5 %
Albino Rabbits
aqueous suspension of gum tragacanth or methyl cellulose, or
3. Statistics should be dissolved or suspended in propylene glycol. Chemi-
cals may also be administered in gelatin capsules.
3.1 Two acceptable procedures are described by (a) Litch-
field and Wilcoxon (1949) (1) where calculation of the LD10,
6. Volume
LD90, and other LD levels is possible; and (b) Thompson
6.1 A similar dosage volume per body weight relationship
(1947) (2), and Thompson and Weil (1952) (3) where only the
should be maintained for each species used.
LD50 can be obtained. Other equally reliable methods may be
6.1.1 The recommended dosage volume per body weight
factor should be 0.1 ml/100 g to 1.0 ml/100 g.
6.1.2 The volume per weight relationship should never
This practice is under the jurisdiction of ASTM Committee E-35 on Pesticides
and is the direct responsibility of Subcommittee E35.17 on Vertebrate Control
exceed 3 ml/100 g body weight under any circumstances.
Agents.
Current edition approved Nov. 10, 1995. Published January 1996. Orignally
7. Animal Type
published as E 555 – 75. Last previous e
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