ASTM E788-97(2013)
(Specification)Standard Specification for Pipet, Blood Diluting
Standard Specification for Pipet, Blood Diluting
ABSTRACT
This specification covers the requirements for glass reusable blood diluting pipets that are used for performing red and white cell corpuscle determinations. The pipets shall be made of common spirit bore white back tubing or clear glass with a white stripe applied to the outer surface of the tubing. The beads shall be made of glass or ceramic composition and shall be of red, clear, or white coloring. Materials shall be tested and the individual types shall conform to specified values of design, capacity, capacity markings, graduation lines, accuracy, workmanship, identification, and pigmentation.
SCOPE
1.1 This specification covers requirements for glass reusable blood diluting pipets that are used for performing red and white cell corpuscle determinations.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation:E788 −97 (Reapproved 2013)
Standard Specification for
Pipet, Blood Diluting
This standard is issued under the fixed designation E788; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5. Dimensions and Permissible Variations
1.1 Thisspecificationcoversrequirementsforglassreusable
5.1 Design—The red and white cell blood dilution pipets
blooddilutingpipetsthatareusedforperformingredandwhite
shall consist of a small uniform bore glass tube which shall
cell corpuscle determinations.
have a bulb of proper size near the proximal end (see Fig. 1).
As an alternative, the pipets may be constructed of three pieces
1.2 The values stated in SI units are to be regarded as
of glass that are fused together to form a one-piece pipet (see
standard. No other units of measurement are included in this
Fig. 2). The bulb shall contain a nonspherical glass or ceramic
standard.
bead sufficiently large enough to prevent its being impacted in
2. Referenced Documents
the constriction portion of the bulb. The cross section of the
2.1 ASTM Standards: pipet at any point shall be circular. The distal end of the white
cell pipet shall be pulled to a point and then ground and
E694 Specification for Laboratory Glass Volumetric Appa-
ratus polished to a tapered tip. The distal end of the red cell pipet
E920 Specification for Commercially Packaged Laboratory shall be ground and polished to a tapered tip. The inside
Apparatus diameter (ID) of the pipet bore at the tip end of the white cell
E921 Specification for Export Packaged Laboratory Appa- pipet shall be 0.2 to 0.5 mm. The external diameter of the
ratus ground and polished tip shall not exceed 2.0 mm. The overall
E1133 Practice for Performance Testing of Packaged Labo- lengthofbothpipetsshallbe104to121mm.Theproximalend
ratory Apparatus for United States Government Procure- may be of funnel design, with an exterior taper, or ground and
ments polishedwithataperofsufficientangletopermitapplicationof
E1157 Specification for Sampling and Testing of Reusable
a rubber suction tube or other suction device (see Fig. 2).
Laboratory Glassware
5.2 Capacity—The capacity of the red cell pipet bulb shall
3. Classification be 0.8 to 1.2 cm and the capacity of the white cell bulb shall be
0.2 to 0.4 cm . The capacity of the red cell pipet stem (bulb to
3.1 This specification covers two different pipet designs and
distal end) shall be 0.008 to 0.012 cm and the capacity of the
permissible alternatives.
white cell pipet stem shall be 0.02 to 0.04 cm .
3.1.1 Red and white cell blood diluting pipets (see Fig. 1).
3.1.2 Permissible alternative designs (see Fig. 2).
5.3 Capacity Markings:
5.3.1 Pipet Bulb Markings—The red and white cell pipet
4. Materials
bulb capacity shall be confined to and indicated by calibration
4.1 The pipets shall be made of common spirit bore white
lines on the pipet. These lines shall be located on both sides of
back tubing or clear glass with a white stripe applied to the
the bulb and shall be within 2 mm to 6 mm above the bulb
outer surface of the tubing.
(proximalend)andwithin3mmto6mmbelowthebulb(distal
4.2 The beads shall be made of glass or ceramic composi- end). The exact manner for measuring these line placements is
tion and shall be of red, clear, or white coloring. specified in Fig. 1.
5.3.2 Pipet Stem Markings—The graduation lines on the
This specification is under the jurisdiction of ASTM Committee E41 on stem of the red and white cell pipets shall be equally divided
Laboratory Apparatusand is the direct responsibility of Subcommittee E41.01 on
into two or ten divisions. If there are two divisions, the two
Apparatus.
calibration lines on the stem will be numbered 0.5 and 1. If
Current edition approved Nov. 1, 2013. Published December 2013. Originally
there are ten divisions, the ten calibration lines will be
approvedin1981.Lastpreviouseditionapprovedin2008asE788 – 97(2008).DOI:
10.1520/E0788-97R13.
numbered 0.5 on the fifth line and 1 on the tenth line
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
respectively. The top line (above bulb at the proximal end)
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
shall be numbered 101 on the red cell pipet and 11 on the white
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. cell pipet, representing stem and bulb volume collectively. At
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E788−97 (2013)
FIG. 1 Pipet Dimensions
the option of the manufacturer, pipet stems may be graduated volumetric tolerances on the pipet. These tolerances may be
with split lines or partial length lines (see Fig. 2). located on the stem or the proximal end of the pipet.
5.7.1 The white cell pipet shall have a clear or white glass
5.4 Graduation Lines—All graduation lines on the red and
or ceramic bead sealed within the bulb to identify it
...
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