ASTM D6363-20

Standard Test Method for Determination of Hydrogen Peroxide and Combined Organic Peroxides in Atmospheric Water Samples by Peroxidase Enzyme Fluorescence Method

SIGNIFICANCE AND USE
5.1 Hydrogen peroxide (formed photochemically in the atmosphere) is a primary oxidizer of dissolved sulfur dioxide in atmospheric water. Detection of H2O2 in atmospheric water is useful for inferring gas-phase H2O2 concentrations and for assessing the relative importance of various acidifying mechanisms under specific atmospheric conditions.  
5.2 Hydroperoxides in samples to be analyzed are unstable in water and can decay rapidly due to bacterial action or chemical reaction with other constituents. The test method includes procedures for sample derivatization and methods for estimating and correcting for hydroperoxide decay.
SCOPE
1.1 This test method covers the determination of hydroperoxides, which include hydrogen peroxide (H2O2) and combined organic peroxides, in samples of atmospheric water by the method of horseradish peroxidase derivatization and fluorescence analysis of the derived dimer.2,3  
1.2 The range of applicable hydrogen peroxide concentrations was determined to be 0.6–176.0 × 10−6 M from independent laboratory tests of the test method.  
1.3 The primary use of the test method is for hydrogen peroxide, but it may also be used to quantitate organic hydroperoxides. Determinations of organic hydroperoxide concentration levels up to 30 × 10−6 M may be adequately obtained by calibration with hydrogen peroxide.2,3 While organic hydroperoxides have not been detected at significant concentration levels in rain or cloud water, their presence may be tested by operation of the test method with the addition of catalase for destruction of H2O2.3  
1.4 Because of the instability of hydroperoxides in atmospheric water samples, proper sample collection, at-collection derivatization, and stringent quality control are essential aspects of the analytical process.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 31-Aug-2020
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: D22 - Air Quality
• Drafting Committee: D22.03 - Ambient Atmospheres and Source Emissions

Relations

• Refers: ASTM D1356-20a - Standard Terminology Relating to Sampling and Analysis of Atmospheres
• Effective Date: 01-Sep-2020
• Refers: ASTM D1356-20 - Standard Terminology Relating to Sampling and Analysis of Atmospheres
• Effective Date: 15-Mar-2020

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D6363 − 20
Standard Test Method for
Determination of Hydrogen Peroxide and Combined Organic
Peroxides in Atmospheric Water Samples by Peroxidase
1
Enzyme Fluorescence Method
This standard is issued under the fixed designation D6363; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope priate safety, health, and environmental practices and deter-
mine the applicability of regulatory limitations prior to use.
1.1 This test method covers the determination of
1.7 This international standard was developed in accor-
hydroperoxides, which include hydrogen peroxide (H O ) and
2 2
dance with internationally recognized principles on standard-
combined organic peroxides, in samples of atmospheric water
ization established in the Decision on Principles for the
by the method of horseradish peroxidase derivatization and
2,3 Development of International Standards, Guides and Recom-
fluorescence analysis of the derived dimer.
mendations issued by the World Trade Organization Technical
1.2 The range of applicable hydrogen peroxide concentra-
Barriers to Trade (TBT) Committee.
−6
tions was determined to be 0.6–176.0 × 10 M from indepen-
dent laboratory tests of the test method. 2. Referenced Documents
4
1.3 The primary use of the test method is for hydrogen
2.1 ASTM Standards:
peroxide, but it may also be used to quantitate organic D1129Terminology Relating to Water
hydroperoxides.Determinationsoforganichydroperoxidecon- D1193Specification for Reagent Water
−6
centration levels up to 30 × 10 M may be adequately D1356Terminology Relating to Sampling and Analysis of
2,3
obtained by calibration with hydrogen peroxide. While Atmospheres
organic hydroperoxides have not been detected at significant D5012Guide for Preparation of Materials Used for the
concentration levels in rain or cloud water, their presence may Collection and Preservation of Atmospheric Wet Deposi-
be tested by operation of the test method with the addition of tion
3
catalase for destruction of H O . D5111Guide for Choosing Locations and Sampling Meth-
2 2
ods to Monitor Atmospheric Deposition at Non-Urban
1.4 Because of the instability of hydroperoxides in atmo-
Locations
spheric water samples, proper sample collection, at-collection
E200Practice for Preparation, Standardization, and Storage
derivatization, and stringent quality control are essential as-
of Standard and Reagent Solutions for ChemicalAnalysis
pects of the analytical process.
1.5 The values stated in SI units are to be regarded as
3. Terminology
standard. No other units of measurement are included in this
3.1 Definitions—For definitions of terms used in this test
standard.
method, refer to Terminologies D1129 and D1356 and Guide
1.6 This standard does not purport to address all of the
D5111.
safety concerns, if any, associated with its use. It is the
3.2 Definitions of Terms Specific to This Standard:
responsibility of the user of this standard to establish appro-
3.2.1 atmospheric water, n—liquidorsolidwatersuspended
in the atmosphere or deposited from the atmosphere. Forms of
atmospheric water include rain, snow, fog, cloud water, dew,
1
This test method is under the jurisdiction of ASTM Committee D22 on Air
and frost.
Quality and is the direct responsibility of Subcommittee D22.03 on Ambient
Atmospheres and Source Emissions.
3.2.2 derivatization, n—formation of the
Current edition approved Sept. 1, 2020. Published September 2020. Originally
p-hydroxyphenylacetic acidic dimer by combination of
approved in 1998. Last previous edition approved in 2013 as D6363–98 (2013).
p-hydroxyphenylacetic acid, horseradish peroxidase reagent,
DOI: 10.1520/D6363-20.
2
Lazrus, A. L., Kok, G. L., Gitlin, S. N., and Lind, J. A., “Automated
Fluorometric Method for Hydrogen Peroxide in Atmospheric Precipitation,” Ana-
4
lytical Chemistry, Vol 57, 1985, pp. 917–922. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
Kok,G.L.,Thompson,K.,andLazrus,A.L.,“DerivatizationTechniqueforthe contactASTM Customer Service at [e-mail]. ForAnnual Book ofASTM
Determination of Peroxides in Precipitation,” Analytical Chemistry, Vol 58, 1986, Standards volume information, refer to the standard’s Document Summary page on
pp. 1192–1194. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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D6363 −
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This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D6363 − 98 (Reapproved 2013) D6363 − 20
Standard Test Method for
Determination of Hydrogen Peroxide and Combined Organic
Peroxides in Atmospheric Water Samples by Peroxidase
1
Enzyme Fluorescence Method
This standard is issued under the fixed designation D6363; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method covers the determination of hydroperoxides, which include hydrogen peroxide (H O ) and combined organic
2 2
peroxides, in samples of atmospheric water by the method of horseradish peroxidase derivatization and fluorescence analysis of
2,3
the derived dimer.
−6
1.2 The range of applicable hydrogen peroxide concentrations was determined to be 0.6–176.0 × 10 M from independent
laboratory tests of the test method.
1.3 The primary use of the test method is for hydrogen peroxide, but it may also be used to quantitate organic hydroperoxides.
−6
Determinations of organic hydroperoxide concentration levels up to 30 × 10 M may be adequately obtained by calibration with
2,3
hydrogen peroxide. While organic hydroperoxides have not been detected at significant concentration levels in rain or cloud
3
water, their presence may be tested by operation of the test method with the addition of catalase for destruction of H O . .
2 2
1.4 Because of the instability of hydroperoxides in atmospheric water samples, proper sample collection, at-collection
derivatization, and stringent quality control are essential aspects of the analytical process.
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.7 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
1
This guide test method is under the jurisdiction of ASTM Committee D22 on Air Quality and is the direct responsibility of Subcommittee D22.03 on Ambient
Atmospheres and Source Emissions.
Current edition approved Oct. 1, 2013Sept. 1, 2020. Published October 2013September 2020. Originally approved in 1998. Last previous edition approved in 20092013
ε1
as D6363 – 98 (2009)(2013). . DOI: 10.1520/D6363-98R13.10.1520/D6363-20.
2
Lazrus, A. L., Kok, G. L., Gitlin, S. N., and Lind, J. A., “Automated Fluorometric Method for Hydrogen Peroxide in Atmospheric Precipitation,” Anal. Chem., 57, 1985,
pp. 917–922.Lazrus, A. L., Kok, G. L., Gitlin, S. N., and Lind, J. A., “Automated Fluorometric Method for Hydrogen Peroxide in Atmospheric Precipitation,” Analytical
Chemistry, Vol 57, 1985, pp. 917–922.
3
Kok, G. L., Thompson, K., and Lazrus, A. L., “Derivatization Technique for the Determination of Peroxides in Precipitation,” Anal. Chem., 58, 1986, pp. 1192–1194.Kok,
G. L., Thompson, K., and Lazrus, A. L., “Derivatization Technique for the Determination of Peroxides in Precipitation,” Analytical Chemistry, Vol 58, 1986, pp. 1192–1194.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

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D6363 − 20
2. Referenced Documents
4
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D1356 Terminology Relating to Sampling and Analysis of Atmospheres
D5012 Guide for Preparation of Materials Used for the Collection and Preservation of Atmospheric Wet Deposition
D5111 Guide for Choosing Locations and Sampling Methods to Monitor Atmospheric Deposition at Non-Urban Locations
E200 Practice for Preparation, Standardization, and Storage of Standard and Reagent Solutions for Chemical Analysis
3. Terminology
3.1 Definitions—For definitions of terms used in this test method, refer to Terminologies D1129 and D1356 and Guide D5111.
3.2 Definitions of Terms Specific to This Standard:
3.2.1 at
...