ASTM E1045-85(1996)
(Specification)Standard Specification for Pipet, Sahli Hemoglobin
Standard Specification for Pipet, Sahli Hemoglobin
SCOPE
1.1 This specification covers reusable pipets calibrated "to contain" 20 cmm of whole blood and used for hemoglobin determinations.
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Designation: E 1045 – 85 (Reapproved 1996)
AMERICAN SOCIETY FOR TESTING AND MATERIALS
100 Barr Harbor Dr., West Conshohocken, PA 19428
Reprinted from the Annual Book of ASTM Standards. Copyright ASTM
Standard Specification for
Pipet, Sahli Hemoglobin
This standard is issued under the fixed designation E 1045; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 4.4 Capacity Deviation—Sahli Hemoglobin pipets are made
with maximum capacity deviation of 6 1.0% or6 2.0 %. The
1.1 This specification covers reusable pipets calibrated“ to
selected capacity deviation shall be marked on the clear or
contain” 20 cmm of whole blood and used for hemoglobin
white stripe portion of the pipet. The capacity of the pipet shall
determinations.
be within the selected capacity deviation marked on the pipet
2. Materials
when tested as specified in 5.2.
2.1 The pipets shall be made of common spirit bore white
5. Testing
back tubing or of clear glass with a white stripe applied to the
5.1 Capacity Test—The capacity of the pipet shall be
outer surface of the tubing.
determined by means of using distilled water and a weighing
3. Design
device with weight sensitivity not less than 0.001 mg.
5.1.1 The pipet shall be thoroughly cleaned, dried, and
3.1 Shape—Pipets shall be straight and of one-piece con-
allowed to adjust to room temperature.
struction. Any cross section of a pipet taken in a plane
5.1.2 The pipet shall be weighed and the weight recorded.
perpendicular to the longitudinal axis should be circular.
5.1.3 The pipet shall be filled to the calibration line with
3.2 Delivery Tips—Delivery tips shall be made with a
distilled water and weighed, and the weight recorded.
gradual or concave taper to a length of 10 to 25 mm. The tip
5.1.4 The recorded weight of the clean and dry pipet shall be
end shall be ground and tapered with fine abrasive, or fire-
subtracted from the recorded weight of the distilled water-filled
polished. Dimensions of the delivery tip shall be as specified in
pipet providing the observed volumetric capacity (V )ofthe
Fig. 1. c
pipet in grams.
3.3 The top of the pipet shall be ground to a taper or formed
5.1.5 The observed volumetric capacity (V ) shall then be
to a funnel shape according to the dimensions specified in Fig. t
corrected to actual volumetric capacity at 20°C (V ), deter-
1. c
mined by:
4. Markings
V
t
V 5 (1)
c
4.1 Graduation Line—The pipet shall have one graduation
1 1 a ~t 2 20°C!
line located 20 cmm from the pipet tip. The graduation line
where:
shall be located on the clear portion of the tubing and shall
V 5 observed volumetric capacity at t °C, grams,
t
extend at least two thirds around the pipet and not exceed 0.4
V 5 corrected volumetric capacity at 20°C,
c
mm
...
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