ASTM E788-97(2003)
(Specification)Standard Specification for Pipet, Blood Diluting
Standard Specification for Pipet, Blood Diluting
ABSTRACT
This specification covers the requirements for glass reusable blood diluting pipets that are used for performing red and white cell corpuscle determinations. The pipets shall be made of common spirit bore white back tubing or clear glass with a white stripe applied to the outer surface of the tubing. The beads shall be made of glass or ceramic composition and shall be of red, clear, or white coloring. Materials shall be tested and the individual types shall conform to specified values of design, capacity, capacity markings, graduation lines, accuracy, workmanship, identification, and pigmentation.
SCOPE
1.1 This specification covers requirements for glass reusable blood diluting pipets that are used for performing red and white cell corpuscle determinations.
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation: E 788 – 97 (Reapproved 2003)
Standard Specification for
Pipet, Blood Diluting
This standard is issued under the fixed designation E 788; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope As an alternative, the pipets may be constructed of three pieces
of glass that are fused together to form a one-piece pipet (see
1.1 Thisspecificationcoversrequirementsforglassreusable
Fig. 2). The bulb shall contain a nonspherical glass or ceramic
blooddilutingpipetsthatareusedforperformingredandwhite
bead sufficiently large enough to prevent its being impacted in
cell corpuscle determinations.
the constriction portion of the bulb. The cross section of the
2. Referenced Documents pipet at any point shall be circular. The distal end of the white
cell pipet shall be pulled to a point and then ground and
2.1 ASTM Standards:
polished to a tapered tip. The distal end of the red cell pipet
E 694 Specification for Volumetric Ware
shall be ground and polished to a tapered tip. The inside
E 920 Specification for Commercially Packaged Laboratory
diameter (ID) of the pipet bore at the tip end of the white cell
Apparatus
pipet shall be 0.2 to 0.5 mm. The external diameter of the
E 921 Specification for Export Packaged Laboratory Appa-
ground and polished tip shall not exceed 2.0 mm. The overall
ratus
lengthofbothpipetsshallbe104to121mm.Theproximalend
E 1133 Practice for PerformanceTesting of Packaged Labo-
may be of funnel design, with an exterior taper, or ground and
ratory Apparatus for United States Government Procure-
polishedwithataperofsufficientangletopermitapplicationof
ments
a rubber suction tube or other suction device (see Fig. 2).
E 1157 Specification for Sampling and Testing of Reusable
5.2 Capacity—The capacity of the red cell pipet bulb shall
Laboratory Glassware
be 0.8 to 1.2 cm and the capacity of the white cell bulb shall be
3. Classification
0.2 to 0.4 cm . The capacity of the red cell pipet stem (bulb to
distal end) shall be 0.008 to 0.012 cm and the capacity of the
3.1 This specification covers two different pipet designs and
white cell pipet stem shall be 0.02 to 0.04 cm .
permissible alternatives.
5.3 Capacity Markings:
3.1.1 Red and white cell blood diluting pipets (see Fig. 1).
5.3.1 Pipet Bulb Markings—The red and white cell pipet
3.1.2 Permissible alternative designs (see Fig. 2).
bulb capacity shall be confined to and indicated by calibration
4. Materials
lines on the pipet. These lines shall be located on both sides of
the bulb and shall be within 2 mm to 6 mm above the bulb
4.1 The pipets shall be made of common spirit bore white
(proximalend)andwithin3mmto6mmbelowthebulb(distal
back tubing or clear glass with a white stripe applied to the
end). The exact manner for measuring these line placements is
outer surface of the tubing.
specified in Fig. 1.
4.2 The beads shall be made of glass or ceramic composi-
5.3.2 Pipet Stem Markings—The graduation lines on the
tion and shall be of red, clear, or white coloring.
stem of the red and white cell pipets shall be equally divided
5. Dimensions and Permissible Variations
into two or ten divisions. If there are two divisions, the two
calibration lines on the stem will be numbered 0.5 and 1. If
5.1 Design—The red and white cell blood dilution pipets
there are ten divisions, the ten calibration lines will be
shall consist of a small uniform bore glass tube which shall
numbered 0.5 on the fifth line and 1 on the tenth line
have a bulb of proper size near the proximal end (see Fig. 1).
respectively. The top line (above bulb at the proximal end)
shall be numbered 101 on the red cell pipet and 11 on the white
This specification is under the jurisdiction of ASTM Committee E41 on
cell pipet, representing stem and bulb volume collectively. At
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
the option of the manufacturer, pipet stems may be graduated
Glass Apparatus.
Current edition approved Nov. 10, 1997. Published February 1998. Originally with split lines or partial length lines (see Fig. 2).
published as E 788 – 81. Last previous edition E 788 – 93.
Annual Book of ASTM Standards, Vol 14.02.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E 788 – 97 (2003)
FIG. 1 Pipet Dimensions
5.4 Graduation Lines—All graduation lines on the red and 5.7.1 The white cell pipet shall have a clear or white glass
white cell pipets shall be at right angles to the pipet axis and or ceramic bead sealed within the bulb to identify it readily as
a white cell pipet.
parallel to each other. The thickness of the graduation lines
shall not exceed 0.4 mm. 5.7.2 The red cell p
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