ASTM D4133-82(2012)
(Practice)Standard Practice for Sampling Phytoplankton with Pumps (Withdrawn 2020)
Standard Practice for Sampling Phytoplankton with Pumps (Withdrawn 2020)
SIGNIFICANCE AND USE
4.1 The advantages of a pumping phytoplankton sampler are as follows:
4.1.1 Sample size is more accurately controlled than with the use of a conical tow net.
4.1.2 Discrete samples from any depth are easily obtained.
4.1.3 Multiple or replicate samples are easily obtained.
4.1.4 They are adaptable to shallow ecosystems.
4.1.5 They allow for a stable collecting efficiency.
4.2 The disadvantages of a pumping phytoplankton sampler are as follows:
4.2.1 They generally are bulky and frequently require an electrical source.
4.2.2 They generally are costly.
4.2.3 They collect only qualitative samples, or semiquantitative samples when used with a volume register.
4.3 There are several special considerations that shall be observed when using a pumping phytoplankton sampler. They are as follows:
4.3.1 Pumps can induce mortality of the organisms and damage the delicate forms.
4.3.2 If tubing is not properly cleaned after use, contamination of subsequent samples can ensue.
SCOPE
1.1 This practice covers the procedures for obtaining qualitative samples of a phytoplankton community by use of pumping systems.
1.2 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
WITHDRAWN RATIONALE
This practice covers the procedures for obtaining qualitative samples of a phytoplankton community by use of pumping systems.
Formerly under the jurisdiction of Committee D19 on Water, this practice was withdrawn in May 2020. This standard was withdrawn without replacement due to its limited use by the industry.
General Information
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D4133 − 82 (Reapproved 2012)
Standard Practice for
1
Sampling Phytoplankton with Pumps
This standard is issued under the fixed designation D4133; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4.1.5 They allow for a stable collecting efficiency.
1.1 This practice covers the procedures for obtaining quali- 4.2 The disadvantages of a pumping phytoplankton sampler
tative samples of a phytoplankton community by use of are as follows:
pumping systems. 4.2.1 They generally are bulky and frequently require an
electrical source.
1.2 This standard does not purport to address all of the
4.2.2 They generally are costly.
safety problems, if any, associated with its use. It is the
4.2.3 They collect only qualitative samples, or semiquanti-
responsibility of the user of this standard to establish appro-
tative samples when used with a volume register.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
4.3 There are several special considerations that shall be
observed when using a pumping phytoplankton sampler. They
2. Referenced Document
are as follows:
2
2.1 ASTM Standards: 4.3.1 Pumps can induce mortality of the organisms and
D4137 Practice for Preserving Phytoplankton Samples
damage the delicate forms.
4.3.2 If tubing is not properly cleaned after use, contamina-
3. Summary of Practice
tion of subsequent samples can ensue.
3.1 Water is pumped from a discrete depth and passed
5. Apparatus
through a net. The captured phytoplankton are removed from
the net and preserved as dictated by the objectives of the study.
5.1 Pumping systems of various kinds have been used to
collect qualitative or semiquantitative samples of phytoplank-
4. Significance and Use
ton. Several papers summarizing these techniques have been
3
,4
4.1 The advantages of a pumping phytoplankton sampler
published. Although a variety of pump apparatus have been
are as follows:
used, the basic design consists of a pump, generally with a
4.1.1 Sample size is more accurately controlled than with
volume register, a base, and a concentrating net, such as a
the use of a conical tow net.
simple tow net sampler or Wisconsin net sampler.
4.1.2 Discrete samples from any depth are easily obtained.
6. Procedure
4.1.3 Multiple or replicate samples are easily obtained.
4.1.4 They are adaptable to shallow ecosystems.
6.1 Pump water from a discrete depth
...
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