Standard Test Method for Quantification of Culturable Waterborne Bacteria Using a Defined Culture Medium Coated Plate

SIGNIFICANCE AND USE
5.1 This plate format is useful for the routine monitoring of culturable, waterborne bacteria in potable and non-potable waters. The significance of finding these bacteria can help with identifying water quality or water system problems or evaluate compliance with maintenance protocols. This test method uses small volumes of water, or dilutions thereof, and provides an easy and reliable method that eliminates media preparation and reduces laboratory waste.
SCOPE
1.1 This test method describes a simple procedure for the quantification of culturable, waterborne bacteria in potable water (drinking water, bottled water, and dental water, for example) and non-potable waters (cooling towers, for example).  
1.1.1 The EasyDisc2, 3 plate format is designed to test 1 mL of a water sample on a 47 mm gridded plate containing a growth reagent embedded to the plate’s inner surface.  
1.1.2 Detection is based on colorimetric technology in which viable, aerobic, heterotrophic, waterborne bacteria grow when present in the water sample, displaying a color reaction which allows for a simplified visualization of colony growth.  
1.2 Each plate can accurately detect up to 300 colony forming units per 1 mL (CFU/1 mL) of sample. To increase the quantification range, a sample dilution can be used. Adjust the CFU/mL result to reflect dilutions.  
1.3 This test method can be used for potable (for example, drinking, bottled, and dental) waters and non-potable waters such as cooling tower waters. It is the user’s responsibility to adhere to all requirements by local regulations and ensure the validity of this test method for waters other than those tested as part of the Interlaboratory Study (ILS).  
1.4 The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Published
Publication Date
30-Jun-2023
Technical Committee
Drafting Committee
Current Stage
Ref Project

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ASTM D8516-23 - Standard Test Method for Quantification of Culturable Waterborne Bacteria Using a Defined Culture Medium Coated Plate
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Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D8516 − 23
Standard Test Method for
Quantification of Culturable Waterborne Bacteria Using a
1
Defined Culture Medium Coated Plate
This standard is issued under the fixed designation D8516; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 1.6 This international standard was developed in accor-
dance with internationally recognized principles on standard-
1.1 This test method describes a simple procedure for the
ization established in the Decision on Principles for the
quantification of culturable, waterborne bacteria in potable
Development of International Standards, Guides and Recom-
water (drinking water, bottled water, and dental water, for
mendations issued by the World Trade Organization Technical
example) and non-potable waters (cooling towers, for ex-
Barriers to Trade (TBT) Committee.
ample).
2,3
1.1.1 The EasyDisc plate format is designed to test 1 mL
2. Referenced Documents
of a water sample on a 47 mm gridded plate containing a
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2.1 ASTM Standards:
growth reagent embedded to the plate’s inner surface.
D1129 Terminology Relating to Water
1.1.2 Detection is based on colorimetric technology in
D1193 Specification for Reagent Water
which viable, aerobic, heterotrophic, waterborne bacteria grow
D2777 Practice for Determination of Precision and Bias of
when present in the water sample, displaying a color reaction
Applicable Test Methods of Committee D19 on Water
which allows for a simplified visualization of colony growth.
D3370 Practices for Sampling Water from Flowing Process
1.2 Each plate can accurately detect up to 300 colony
Streams
forming units per 1 mL (CFU/1 mL) of sample. To increase the
5
2.2 ISO Standard:
quantification range, a sample dilution can be used. Adjust the
ISO 6222 Water quality—Enumeration of culturable micro-
CFU/mL result to reflect dilutions.
organisms—Colony count by inoculation in a nutrient
1.3 This test method can be used for potable (for example,
agar culture medium
drinking, bottled, and dental) waters and non-potable waters
2.3 Standard Methods for the Examination of Water and
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such as cooling tower waters. It is the user’s responsibility to
Wastewater:
adhere to all requirements by local regulations and ensure the
9060 Samples
validity of this test method for waters other than those tested as
9215 Heterotrophic Plate Count
part of the Interlaboratory Study (ILS).
3. Terminology
1.4 The values stated in SI units are to be regarded as the
standard. No other units of measurement are included in this 3.1 Definitions:
standard. 3.1.1 For definitions of terms used in this test method, refer
to Terminology D1129.
1.5 This standard does not purport to address all of the
3.1.2 ambient temperature, n—temperature of the
safety concerns, if any, associated with its use. It is the
surroundings, generally assumed to be 20 °C to 25 °C.
responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter- 3.1.3 colony forming unit, CFU, n—in microbiology, a
mine the applicability of regulatory limitations prior to use. visible mass of cells (algae, bacteria, or fungi) originating from
either an individual cell or cluster of cells that have been placed
onto or dispersed into a solid or semi-solid nutrient medium
1 and subsequently incubated under prescribed conditions.
This test method is under the jurisdiction of ASTM Committee D19 on Water
and is the direct responsibility of Subcommittee D19.24 on Water Microbiology.
Current edition approved July 1, 2023. Published August 2023. DOI: 10.1520/
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D8516-23. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
2
EasyDisc is a registered trademark of IDEXX Laboratories, Inc. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
3
The sole source of supply of the apparatus known to the committee at this time Standards volume information, refer to the standard’s Document Summary page on
is IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME, 04092, USA. If the ASTM website.
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you are aware of alternative suppliers, please provide this information to ASTM Available from American National Standards Institute (ANSI), 25 W. 43rd St.,
International Headquarters. Your comments will receive careful consideration at a 4th Floor, New York, NY 10036, http://www.ansi.org.
1 6
meeting of the responsible technical committee, which you may attend. Available online from https:/
...

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