ASTM E193-94(1999)
(Specification)Standard Specification for Laboratory Glass Micropipets
Standard Specification for Laboratory Glass Micropipets
SCOPE
1.1 This specification covers several micropipets useful in measuring microlitre quantities of liquids. Note-This specification was originally developed by the Committee on Microchemical Apparatus, Division of Analytical Chemistry, American Chemical Society.
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Designation: E 193 – 94 (Reapproved 1999)
Standard Specification for
Laboratory Glass Micropipets
This standard is issued under the fixed designation E 193; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope shown in Fig. 4 are listed as micropipets, density-type, de-
signed for use as microweighing pipets, or pycnometers.
1.1 This specification covers several micropipets useful in
measuring microlitre quantities of liquids.
4. Micropipets, Measuring-Type (“to deliver”)
,
NOTE 1—ThisspecificationwasoriginallydevelopedbytheCommittee 2 6
4.1 The measuring-type micropipets are specified in five
onMicrochemicalApparatus,DivisionofAnalyticalChemistry,American
capacities and shall conform to the requirements shown in Fig.
2,3,4
Chemical Society.
1. The narrow, elongated delivery stem of the pipets can reach
2,5
to the bottom of microvolumetric flasks, permitting almost
2. Referenced Documents
complete withdrawal of the contents of the flask. Quantitative
2.1 ASTM Standards:
delivery of the entire volume of the pipets is obtained by
E 920 Specification for Commercially Packaged Laboratory
touching off the last drop.
Apparatus
E 921 Specification for Export Packaged Laboratory Appa-
5. Micropipets, Folin-Type (“to contain”)
ratus
4,6,7
5.1 The Folin-type micropipets are specified in two
E 1133 PracticeforPerformanceTestingofPackagedLabo-
capacities and shall conform to the requirements shown in Fig.
ratoryApparatusUnitedStatesGovernmentProcurements
2.Completedeliveryofthevolumeindicatedbythegraduation
E 1157 Specification for Sampling and Testing Reusable
mark on these pipets is ensured by washing out several times
Laboratory Glassware
any solution adhering to the inner surface with wash liquid
drawn up from the tip.
3. Nomenclature
3.1 ThepipetsspecifiedinFigs.1-3,andFig.4areclassified
6. Micro Washout Pipets, Pregl-Type (“to contain”)
as micropipets, whereas those in Figs. 5 and 6 are classified as
, ,
4 6 8
6.1 The micro washout pipets, Pregl-type are specified
microlitre pipets in order to differentiate among the various
in four capacities and shall conform to the requirements shown
pipets,notonatechnicalbasis,butratheronthebasisofusage.
in Fig. 3. Quantitative delivery of the volume indicated by the
This is done to prevent confusion that might result from the
graduation mark on the pipets is obtained by rinsing out the
fact that the pipets shown in Figs. 1 and 2, and Fig. 3 are listed
contents with wash liquid added from the top of the pipet.
in trade catalogs as micropipets designed especially for use in
the fields of biological and clinical chemistry, and the pipets
7. Micro Weighing Pipets, Density-Type (Pycnometers)
(“to contain”)
7.1 The micro weighing pipets, density-type (pycnom-
This specification is under the jurisdiction of ASTM Committee E-41 on
, ,
4 6 9
eters), with ground-glass caps, are specified in three ca-
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
Glass Apparatus. pacities and shall conform to the requirements shown in Fig. 4.
Current edition approved Feb. 15, 1994. Published April 1994. Originally
These pipets are designed for density determinations on small
published as E 193 – 62 T. Last previous edition E 193 – 64 (1988).
amounts of volatile, viscous or hygroscopic liquids. The
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
decigram size has a capacity of 100 µL and is especially
American Chemical Society. “Report on Recommended Specifications for Micro-
chemical Apparatus, Volumetric Glassware, Flasks, Pipets, and Centrifuge Tubes.”
suitable for highly viscous liquids. The centigram size has a
Analytical Chemistry, Vol 28, 1956, p. 1993.
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
American Chemical Society. “Report on Recommended Specifications for Micro-
chemical Apparatus, Volumetric Glassware, Microlitre Pipets.” Analytical Chemis- Steyermark,A., Quantitative Organic Microanalysis, 2nd Ed.,Academic Press,
try, Vol 30, 1958, p. 1702. New York, NY, 1961.
4 7
Committee on Microchemical Apparatus, Division of Analytical Chemistry, Folin, O., Journal of Biological Chemistry, Vol 77, 1928, p. 421.
American Chemical Society. “Report on Recommended Specifications for Micro- Grant,J.,QuantitativeOrganicMicroanalysisBasedonMethodsofFritzPregl,
chemical Apparatus, Volumetric Glassware, Micropipets.” Analytical Chemistry, 5th Ed., Blakiston Co., Philadelphia, PA 1951, p. 34.
Vol 32, 1960, p. 1045. Alber, H. K., Industrial and Engineering Chemistry,Analytical Edition,Vol 12,
Annual Book of ASTM Standards, Vol 14.04. 1940, p. 764.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E 193
Lining
Subdivision, Interval Graduated, Ring at Each, Half Ring Number at 0 Tolerance, 6,
Capacity, mL mL mL mL at Each, mL and Each mL mL
0.2 0.01 0 to 0.18 0.02 0.01 0.02 0.005
0.5 0.01 0 to 0.45 0.05 0.01 0.1 0.01
1 0.02 0 to 0.90 0.1 0.02 0.1 0.02
2 0.05 0 t
...
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