ASTM E193-94(2005)

Standard Specification for Laboratory Glass Micropipets

ABSTRACT
This specification describes the design and dimensional requirements for several types of laboratory glass micropipets useful in measuring microlitre quantities of liquids. The types of micropipets covered here are the measuring-type micropipets, Folin-type micrpipets, Pregl-type micro washout pipets, density-type micro weighing pipets, and microlitre pipets.
SCOPE
1.1 This specification covers several micropipets useful in measuring microlitre quantities of liquids. Note 1This specification was originally developed by the Committee on Microchemical Apparatus, Division of Analytical Chemistry, American Chemical Society.,,

General Information

• Status: Historical
• Publication Date: 30-Apr-2005
• ICS: 71.040.20 - Laboratory ware and related apparatus
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Relations

• Replaces: ASTM E193-94(1999) - Standard Specification for Laboratory Glass Micropipets
• Effective Date: 01-May-2005
• Replaced By: ASTM E193-94(2010) - Standard Specification for Laboratory Glass Micropipets
• Effective Date: 01-Jul-2010
• Referred By: ASTM E969-02(2019) - Standard Specification for Glass Volumetric (Transfer) Pipets
• Effective Date: 01-May-2005

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E193 – 94 (Reapproved 2005)
Standard Specification for
Laboratory Glass Micropipets
This standard is issued under the fixed designation E193; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope This is done to prevent confusion that might result from the
fact that the pipets shown in Figs. 1 and 2, and Fig. 3 are listed
1.1 This specification covers several micropipets useful in
in trade catalogs as micropipets designed especially for use in
measuring microlitre quantities of liquids.
the fields of biological and clinical chemistry, and the pipets
NOTE 1—ThisspecificationwasoriginallydevelopedbytheCommittee
shown in Fig. 4 are listed as micropipets, density-type, de-
on MicrochemicalApparatus, Division ofAnalytical Chemistry,American
signed for use as microweighing pipets, or pycnometers.
2,3,4
Chemical Society.
4. Micropipets, Measuring-Type (“to deliver”)
2. Referenced Documents
,
2 6
4.1 The measuring-type micropipets are specified in five
2.1 ASTM Standards:
capacities and shall conform to the requirements shown in Fig.
E920 Specification for Commercially Packaged Laboratory
1. The narrow, elongated delivery stem of the pipets can reach
Apparatus
2,5
to the bottom of microvolumetric flasks, permitting almost
E921 Specification for Export Packaged Laboratory Appa-
complete withdrawal of the contents of the flask. Quantitative
ratus
delivery of the entire volume of the pipets is obtained by
E1133 Practice for Performance Testing of Packaged Labo-
touching off the last drop.
ratory Apparatus for United States Government Procure-
ments
5. Micropipets, Folin-Type (“to contain”)
E1157 Specification for Sampling and Testing of Reusable
4,6,7
5.1 The Folin-type micropipets are specified in two
Laboratory Glassware
capacities and shall conform to the requirements shown in Fig.
2.Completedeliveryofthevolumeindicatedbythegraduation
3. Nomenclature
mark on these pipets is ensured by washing out several times
3.1 ThepipetsspecifiedinFigs.1-3,andFig.4areclassified
any solution adhering to the inner surface with wash liquid
as micropipets, whereas those in Figs. 5 and 6 are classified as
drawn up from the tip.
microlitre pipets in order to differentiate among the various
pipets,notonatechnicalbasis,butratheronthebasisofusage.
6. Micro Washout Pipets, Pregl-Type (“to contain”)
, ,
4 6 8
6.1 The micro washout pipets, Pregl-type are specified
in four capacities and shall conform to the requirements shown
This specification is under the jurisdiction of ASTM Committee E41 on
in Fig. 3. Quantitative delivery of the volume indicated by the
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
Apparatus.
graduation mark on the pipets is obtained by rinsing out the
Current edition approved May 1, 2005. Published October 2005. Originally
contents with wash liquid added from the top of the pipet.
approved in 1962. Last previous edition approved in 1999 as E193 – 94 (1999).
DOI: 10.1520/E0193-94R05.
2 7. Micro Weighing Pipets, Density-Type (Pycnometers)
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
(“to contain”)
American Chemical Society. “Report on Recommended Specifications for Micro-
chemical Apparatus, Volumetric Glassware, Flasks, Pipets, and Centrifuge Tubes.”
7.1 The micro weighing pipets, density-type (pycnom-
Analytical Chemistry, Vol 28, 1956, p. 1993.
4,6,9
eters), with ground-glass caps, are specified in three ca-
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
American Chemical Society. “Report on Recommended Specifications for Micro-
pacities and shall conform to the requirements shown in Fig. 4.
chemical Apparatus, Volumetric Glassware, Microlitre Pipets.” Analytical Chemis-
try, Vol 30, 1958, p. 1702.
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
American Chemical Society. “Report on Recommended Specifications for Micro- Steyermark, A., Quantitative Organic Microanalysis , 2nd Ed., Academic
chemical Apparatus, Volumetric Glassware, Micropipets.” Analytical Chemistry, Press, New York, NY, 1961.
Vol 32, 1960, p. 1045. Folin, O., Journal of Biological Chemistry, Vol 77 , 1928, p. 421.
5 8
For referenced ASTM standards, visit the ASTM website, www.astm.org, or Grant, J., Quantitative Organic Microanalysis Based on Methods of Fritz
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Pregl, 5th Ed., Blakiston Co., Philadelphia, PA 1951, p. 34.
Standards volume information, refer to the standard’s Document Summary page on Alber, H. K., Industrial and Engineering Chemistry, Analytical Edition,Vol
the ASTM website. 12, 1940, p. 764.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E193 – 94 (2005)
Lining
Subdivision, Interval Graduated, Ring at Each, Half Ring Number at 0 Tolerance, 6,
Capacity, mL mL mL mL at Each, mL and Each mL mL
0.2 0.01 0 to 0.18 0.02 0.01 0.02 0.005
0.5 0.01 0 to 0.45 0.05 0.01 0.1 0.01
1 0.02 0 to 0.90 0.1 0.02 0.1 0.02
2 0.05 0 to 1.75 0.25 0.05 0.5 0.04
3 0.05 0 to 2.70 0.25 0.05 0.5 0.06
Notes:
1. No graduations shall appear in tapered portion.
2. Tip may be tapered at junction with body, but outside diameter at this poin
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