ASTM E1044-96(2011)
(Specification)Standard Specification for Glass Serological Pipets (General Purpose and Kahn)
Standard Specification for Glass Serological Pipets (General Purpose and Kahn)
ABSTRACT
This specification covers reusable glass serological pipets used for measuring volumes of liquid. The pipets may be classified into three styles according to operational set-up and should be made with approved glass materials. Each pipet should be straight, of one-piece construction, and properly calibrated. All products should conform to the required dimension of delivery tips, zero gradation line position, dimensions and outflow times, graduation markings, color coding, identification markings, and workmanship.
SCOPE
1.1 This specification covers glass serological pipets, used in measuring volumes of liquids.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation:E1044 −96 (Reapproved 2011)
Standard Specification for
Glass Serological Pipets (General Purpose and Kahn)
This standard is issued under the fixed designation E1044; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4. General Requirements
4.1 Borosilicate Glass—Borosilicate glass for pipets shall
1.1 This specification covers glass serological pipets, used
conform to the glass requirements of Type 1, Class A or B of
in measuring volumes of liquids.
Specification E438.
2. Referenced Documents
4.2 Calibration—Pipets shall be calibrated in accordance
with Practice E542 to deliver (TD) the intended capacity of
2.1 ASTM Standards:
distilled water at 20°C when the last drop is blown out, except
E438 Specification for Glasses in Laboratory Apparatus
the 0.25 mL Kahn pipet; this Kahn pipet shall have a clear
E542 Practice for Calibration of Laboratory Volumetric
ungraduated space of not less than 63 mm between the
Apparatus
lowermost graduation mark and the tip of the pipet. The pipet
E671 Specification for Maximum Permissible Thermal Re-
shall be filled about 10 mm above the capacity line. Remove
sidual Stress in Annealed Glass Laboratory Apparatus
any water on the outside of the tip by a downward wipe with
E694 Specification for Laboratory Glass Volumetric Appa-
filter paper. Next, place the tip in contact with the wetted wall
ratus
of a beaker and slowly lower the meniscus to the capacity line.
E920 Specification for Commercially Packaged Laboratory
Do not remove any water remaining on the tip at this time.
Apparatus
Delivery of the contents into a receiving vessel is made with
E921 Specification for Export Packaged Laboratory Appa-
the tip in contact with the wall of the vessel. When the water
ratus
has ceased to flow, the water remaining in the tip is then blown
E1133 Practice for Performance Testing of Packaged Labo-
out with one firm puff with the tip in contact with the wall of
ratory Apparatus for United States Government Procure-
the vessel, if possible. No after-drainage period is allowed.
ments
Capacity tolerances shall be within the limits given in Table 1.
E1157 Specification for Sampling and Testing of Reusable
4.3 Annealing—The maximum residual thermal stress shall
Laboratory Glassware
be such as to conform to Specification E671, except that
tempered tips or tempered tops, or both, may be supplied at the
3. Classification
option of the manufacturer. The tempered tip, when examined
3.1 Style and Capacity—Pipets covered by this specification
in index oil that matches the refractive index of the glass being
shall be of the following styles and capacities: used, shall have a temper between 75 and 220 nm.
3.1.1 Style I—General purpose top end.
5. Design
3.1.2 Style II—Cotton plugging top end.
5.1 Shape—The pipets shall be straight and of one-piece
3.1.3 Style III—Cotton plugging top end, large tip opening.
construction. Any cross section of a pipet taken in a plane
3.1.4 Capacities (mL)—0.1; 0.1 (Kahn); 0.2; 0.2 (Kahn);
perpendicular to the longitudinal axis shall be circular.
0.25 (Kahn); 0.5; 0.60 (Kahn); 1.0; 2.0; 5.0; 10.0; and 25.0.
5.2 Delivery Tips—Delivery tips shall be made with a
gradual taper of 15 to 30 mm for capacities up to 5 mL
inclusive, and 20 to 35 mm for 10 and 25 mL capacity pipets.
This specification is under the jurisdiction of ASTM Committee E41 on
The end of the tip shall be perpendicular to the longitudinal
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
Apparatus.
axis of the pipet. Sudden constriction at the orifice shall not be
Current edition approved Dec. 1, 2011. Published December 2011. Originally
acceptable. The outside edge of the tip shall be bevelled
approved in 1985. Last previous edition approved in 2006 as E1044 – 96 (2006).
slightly at the end, and the bevel shall be ground or firepol-
DOI: 10.1520/E1044-96R11.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or ished.
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
5.3 Zero Graduation Line Position—The distance from the
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. top end to the top graduation of all pipets (except for 0.5 mL
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E1044−96 (2011)
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