ASTM D5583-06
(Test Method)Standard Test Method for Detection and Estimation of Retention of Wood Preservatives by Aspergillus Bioassaying (Withdrawn 2010)
Standard Test Method for Detection and Estimation of Retention of Wood Preservatives by Aspergillus Bioassaying (Withdrawn 2010)
SIGNIFICANCE AND USE
This test method is intended for use with any preservative that is of uniform composition and not so tightly bound to the wood or so water insoluble that it cannot diffuse in the culture medium on which the assay fungus is grown (1, 2, 3).2 It is especially applicable to monitoring non-pressure treated millwork and other wood items (4), but it can be used to determine approximate preservative retentions at prescribed locations in pressure-treated wood (5). When measuring longitudinal preservative penetration, as is especially useful with millwork, the wood can be sampled on the end grain. The assay also provides a reliable means of observing losses of preservative retention in wood during service (or experimentally subjected to particular environmental conditions). This test method is not intended for comparing preservatives nor for estimating retentions of a preservative of variable composition, such as creosote. It can be used, however, for comparing relative potencies of such preservative.
SCOPE
1.1 This test method covers a non-chemical, biological procedure for assaying wood for approximate preservative content (Note 0). It requires minimal equipment and technical proficiency. No costly equipment is needed other than a small autoclave for preparing fungal media. Since this test method does not require composite samples to ascertain preservation retentions, therefore it is used to assess retention at individual locations.Note 0
With appropriate, simple adaptation, the method can be used with other products besides wood, such as fungicidal paints and glues. The assaying is done wherever it is convenient, since aseptic precautions are not required except for maintaining a pure stock culture of the assay fungus.
1.2 The values stated in SI units are to be regarded as the standard. The inch-pound units given in parentheses are for information only.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
WITHDRAWN RATIONALE
This test method covers a non-chemical, biological procedure for assaying wood for approximate preservative content.
Formerly under the jurisdiction of Committee D07 on Wood, this test method was withdrawn in March 2010. This standard is being withdrawn without replacement because it is no longer used in the industry.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information.
Designation: D5583 – 06
Standard Test Method for
Detection and Estimation of Retention of Wood
1
Preservatives by Aspergillus Bioassaying
This standard is issued under the fixed designation D5583; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope preservative,fromassaysofspecimenscontainingagradientof
retentions of the preservative. (See example for pentachlo-
1.1 This test method covers a non-chemical, biological
rophenol in Fig. 3.) Hereafter, this is called the reference
procedure for assaying wood for approximate preservative
relations.
content (Note 1). It requires minimal equipment and technical
proficiency. No costly equipment is needed other than a small
3. Significance and Use
autoclave for preparing fungal media. Since this test method
3.1 This test method is intended for use with any preserva-
does not require composite samples to ascertain preservation
tive that is of uniform composition and not so tightly bound to
retentions, therefore it is used to assess retention at individual
the wood or so water insoluble that it cannot diffuse in the
locations.
2
culture medium on which the assay fungus is grown (1, 2, 3).
NOTE 1—With appropriate, simple adaptation, the method can be used
It is especially applicable to monitoring non-pressure treated
withotherproductsbesideswood,suchasfungicidalpaintsandglues.The
millwork and other wood items (4), but it can be used to
assaying is done wherever it is convenient, since aseptic precautions are
determine approximate preservative retentions at prescribed
not required except for maintaining a pure stock culture of the assay
locations in pressure-treated wood (5). When measuring lon-
fungus.
gitudinal preservative penetration, as is especially useful with
1.2 The values stated in SI units are to be regarded as the
millwork,thewoodcanbesampledontheendgrain.Theassay
standard. The inch-pound units given in parentheses are for
also provides a reliable means of observing losses of preser-
information only.
vative retention in wood during service (or experimentally
1.3 This standard does not purport to address all of the
subjected to particular environmental conditions). This test
safety concerns, if any, associated with its use. It is the
method is not intended for comparing preservatives nor for
responsibility of the user of this standard to establish appro-
estimating retentions of a preservative of variable composition,
priate safety and health practices and determine the applica-
such as creosote. It can be used, however, for comparing
bility of regulatory limitations prior to use.
relative potencies of such preservative.
2. Summary of Test Method
4. Apparatus
2.1 Small wood specimens of prescribed size are placed on
4.1 Conventional Equipment, for culturing and aseptic han-
nutrient agar in a Petri dish freshly seeded with spores of
dling of fungi, such as autoclave, refrigerator, transfer needle,
Aspergillus niger, a black mold, and the culture is allowed to
and gas or alcohol burner.
incubateforthreetofourdays.Thefungusisunabletoproduce
4.2 Glassware, 100 by 15 mm (approximately) Petri dishes,
its typical black spores around samples containing an inhibi-
glass or plastic, 250 mL Erlenmeyer flasks, and a few 150 by
tory amount of preservative; this indicates the presence of
22 mm (approximately) test tubes.
preservative, and the size of the white area (zone of effect) is
4.3 RoomorChamber,inwhichtemperatureiscontrolledat
the index of preservative retention (see Fig. 1 and Fig. 2). The
22 to 27°C (72 to 82°F).
relation of retention to zone of effect is determined for each
4.4 Atomizer, delivering a fine spray (optional).
4.5 Tools, such as increment borer, plug cutter, saw, and
1
This test method is under the jurisdiction of ASTM Committee D07 on Wood knife for extracting samples and cutting them to desired
and is the direct responsibility of Subcommittee D07.06 on Treatments for Wood
specimen size.
Products.
Current edition approved March 1, 2006. Published March 2006. Originally
2
approved in 1994. Last previous edition approved in 2000 as D5583 – 00. DOI: The boldface numbers in parentheses refer to the list of references at the end of
10.1520/D5583-06. this test method.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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D5583 – 06
NOTE 1—The black area shows dense coverage by black spores of the fungus j 6.
FIG. 1 Zone of Effect (d) in an Aspergillus Bioassay of a Pentachlorophenol-Treated Block
NOTE 1—Penta retentions (
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