ASTM E652-91(2009)

Standard Test Method for Nonresidual Liquid Household Insecticides Against Flying Insects (Withdrawn 2018)

SIGNIFICANCE AND USE
This test method provides a satisfactory means of determining the relative efficacy of spray formulations against house flies (Musca domestica, L).
Test data obtained by this test method may also be adequate to support label claims for the use of the product against mosquitoes, gnats, flying moths, wasps, and certain other small flying insects. This test method is not designed to measure the residual action of the spray formulation.
As a biological test, it is subject to the variations that accompany the reactions of living organisms. It should be employed under the supervision of personnel familiar with the biological testing of insecticides.
SCOPE
1.1 This test method covers the determination of the relative efficiency of household and industrial-use, contact insecticides dissolved in base oils.
1.2 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
WITHDRAWN RATIONALE
This test method covers the determination of the relative efficiency of household and industrial-use, contact insecticides dissolved in base oils.
Formerly under the jurisdiction of Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents, this test method was withdrawn in January 2018 in accordance with section 10.6.3 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

General Information

• Status: Withdrawn
• Publication Date: 31-Mar-2009
• Withdrawal Date: 18-Jan-2018
• ICS: 71.100.99 - Other products of the chemical industry
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents

Relations

• Replaces: ASTM E652-91(2003) - Standard Test Method for Nonresidual Liquid Household Insecticides Against Flying Insects
• Effective Date: 01-Apr-2009

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E652 − 91 (Reapproved 2009)
Standard Test Method for
Nonresidual Liquid Household Insecticides Against Flying
Insects
This standard is issued under the fixed designation E652; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope basis for evaluating the efficacy of the test insecticide by the
small and large group test methods.
1.1 Thistestmethodcoversthedeterminationoftherelative
efficiency of household and industrial-use, contact insecticides
4. Significance and Use
dissolved in base oils.
4.1 This test method provides a satisfactory means of
1.2 The values stated in inch-pound units are to be regarded
determining the relative efficacy of spray formulations against
as standard. The values given in parentheses are mathematical
house flies (Musca domestica, L).
conversions to SI units that are provided for information only
4.2 Test data obtained by this test method may also be
and are not considered standard.
adequate to support label claims for the use of the product
1.3 This standard does not purport to address all of the
against mosquitoes, gnats, flying moths, wasps, and certain
safety concerns, if any, associated with its use. It is the
other small flying insects. This test method is not designed to
responsibility of the user of this standard to establish appro-
measure the residual action of the spray formulation.
priate safety and health practices and determine the applica-
4.3 As a biological test, it is subject to the variations that
bility of regulatory limitations prior to use.
accompany the reactions of living organisms. It should be
employed under the supervision of personnel familiar with the
2. Terminology
biological testing of insecticides.
2.1 Definitions:
2.1.1 culture, n—all adult flies resulting from the seeding of
5. Apparatus
eggs collected at one time on a given date.
5.1 CSMA Pesticide Atomizer, fitted with a No. 631 cut off
2.1.2 knocked-down—pertainingtoalltestfliesincapableof
and a glass reservoir.
coordinated movement (moribund).
5.2 Rearing Room—Aroom of any convenient size, free of
strong drafts, and maintained at 80 6 2°F (27 6 1°C) with a
3. Summary of Test Method
relative humidity of 50 6 5%. This room must be separate
3.1 Two methods for evaluating liquid household insecti-
from the testing room and ventilated to minimize odors.
cides are permitted as follows:
2 5.3 Testing Room, maintained at 80 6 2°F (27 6 1°C) and
3.1.1 For the small group method, a minimum of 10
a relative humidity of 50 6 5%. This room may be of any
replicatesofapproximately100flieseachareexposedtoatotal
3 convenient size capable of holding the standard Peet-Grady
of 12 cm of test insecticide per replicate.
chamber with adequate additional space to permit efficient
3.1.2 For the large group procedure, use two separate fly
performance of the test.
cultures, four randomized tests with 500 flies per replicate
using 10 replicates. 5.4 Peet-Grady Test Chamber (see Annex A1.).
3.2 The difference in percentage mortality of the Official 5.5 Cylindrical Glass Battery Jars, 6 in. (150 mm) in
Test Insecticide (OTI) (see 8.2.1) and the test insecticide is the diameter and 9 in. (230 mm) high, or other suitable containers,
to be used as fly larval medium containers.
5.6 Calibrated Pipet, or graduate with 0.1-cm graduations.
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
responsibility of Subcommittee E35.12 on Insect Control Agents. The sole source of supply of the apparatus known to the committee at this time
Current edition approved April 1, 2009. Published April 2009. Originally is Chemical Specialties Manufacturers Assn. (CSMA), 1913 Eye St., N.W.,
approvedin1978.Lastpreviouseditionapprovedin2003asE652–91(2003).DOI: Washington,DC20006.Ifyouareawareofalternativesuppliers,pleaseprovidethis
10.1520/E0652-91R09. information to ASTM International Headquarters. Your comments will receive
2 1
“Peet-Grady Method,” Official Method of the Chemical Specialties Manufac- careful consideration at a meeting of the responsible technical committee, which
turers Association for Evaluating Liquid Household Insecticides. you may attend.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E652 − 91 (2009)
5.7 Electric Fan. 8.2 Reference Standards:
8.2.1 Current Offıcial Test Insecticide (OTI).
5.8 Air Separation Apparatus for Recovering Puparia, con-
structed according to the specifications of Goodhue and Lin-
9. Procedure
nard.
9.1 House Fly Rearing Technique:
3 3
5.9 Fly Cages, providing at least 1 in. (16.4 cm ) of space
9.1.1 Larval Medium—Mix the larval medium (see 6.2)
per fly with a minimum of two sides and the top screened.
thoroughlyuntilaloose,fluffyconsistencyisobtained,transfer
Cages shall be constructed of metal or other suitable material
it to the battery jar (or other container) without packing, cover
and fitted with a sleeve opening, rubber membrane, or a door.
withasuitablecover,andplaceintheinsectary.Theamountof
A detachable floor is preferable to facilitate cleaning and
suspension required for best rearing results will need to be
insertion of a paper floor covering.
determined in each laboratory and it may be varied to prevent
mold growth. It is suggested that the medium be prepared in
6. Reagents and Materials
the late afternoon of the day before egg collection.
6.1 Adult Fly Food—5% spray-dried (or instant) nonfat
9.1.2 Eggs—Collect eggs for a period not longer than 16 h
milk solids and 2% granulated sugar dissolved in water (40%
from food dishes or other oviposition medium in cages
formalinsolutionmaybeaddedattherateof1+1500todelay
containing mature flies not more than 8 days old. It is
spoiling). Each cage requires 15 cm of food per 100 flies per
suggested that fresh oviposition medium be placed in fly cages
day.
in the late afternoon for egg collection early on the following
morning. Measure and seed the collected eggs without delay.
6.2 Larval Medium—340 g of CSMA Standard Fly Me-
6 3
Wash all the eggs together in tap water at room temperature
dium added to 750 cm of an aqueous suspension containing
and measure groups of 2000 as accurately as possible. This
15 g of moist cake yeast (or5gofdry yeast) and 10 cm of
maybedonebyallowingtheeggstosettleinacalibratedpipet
nondiastatic diamalt per container (see 5.5). Some modifica-
or graduate (0.1 cm of settled eggs is approximately 700), or
tions in liquid content may be needed to give maximum larval
theeggscanbefilteredandmeasuredincalibratedpitsorcells.
production.
Use 10 cm of tap water to measure and to scatter the eggs in
6.3 Puparial Medium—An added 2-in. (51-mm) layer of
a pit or trench 0.5 in. (13 mm) deep which is located in the
vermiculite on the dry top surface of the fly larval medium.
centerofthesurfaceofthelarvalmedium.Covertheeggswith
loosemediumandplacethecoveredcontainersintheinsectary
7. Test Specimen and Sample
with at least 1.5-in. (38-mm) separation to permit free air
7.1 The test insect must be the adult house fly (Musca
circulation.The maximum temperature in the jar (about 3 days
domestica L) reared from the current CSMA official resistant
later) must not exceed 130°F (54.4°C). Under normal condi-
house-fly strain.
tions more than 85% of the eggs should hatch within 36 h of
7.2 Adult house flies in test groups must be between 3 and
the time they are laid.
6 days of age at the time of testing.
9.1.3 Pupae—Approximately3to4daysaftertheeggshave
been seeded, a 2-in. (51-mm) layer of vermiculite may be
8. Calibration and Standardization
added on the surface of the larval medium to aid in pupae
8.1 Apparatus:
recovery. Mature larvae migrate to the top portion of the
8.1.1 Atomizer—Maintain pressure at a constant 12.5 6 0.5
mediumortothevermiculitelayer,andnormallyalllarvaewill
psi (86.2 6 3.4 kPa) as measured by a gage of not more than
have pupated about 9 days after seeding the eggs. When this
30-psi (207-kPa) capacity or a manometer. Calibrate the
occurs, the portion containing pupae may be removed, poured
atomizer at 80 6 2°F (27 6 1°C) to deliver 12 cm of OTI in
into a shallow tray, and air-dried at room temperature. An
24 61s.
electric fan may be used to hasten drying. Then separate the
8.1.2 Test Chamber Contamination—Consider chambers
pupae from the dry medium or the vermiculite. Handle gently
contaminated and unsatisfactory for use when test flies (3 to 6
and as little as possible to avoid injury to the pupae. Any
daysold)heldinthechamberfora12to16-hperiodwithfood,
method that permits at least 90% of the flies to emerge is
butwithoutinsecticidetreatment,showmortalitiesgreaterthan
considered satisfactory.
10%, or when over 10% of the flies are paralyzed within 30 9.1.3.1 Air-Separation Apparatus—An air-separation appa-
min after liberation.
ratus (see 5.8) is used by several laboratories for cleaning
pupae and has been found to be more rapid than the indicated
traymethod.Thedeviceemploysablower,acyclonecollector,
Goodhue, L. D., and Linnard, C. E., “Air Separation Apparatus for Cleaning
andasuctionpipetoseparatetheheavierpupaefromalayerof
Fly Pupae,” Journal of Economic Entomology, Vol 43, 1950, p. 228.
5 7
The sole source of supply of the apparatus known to the committee at this time
vermiculite placed on the surface of the fly larval medium.
is American Biological Supply Co., 1330 Dillon Heights Ave., Baltimore, MD
9.1.3.2 Combine all of the pupae maturing on a given day
21228. If you are aware of alternative suppliers, please provide this information to
intoonelot,mix,andmeasureintotestunitgroups.Eachgroup
ASTM International Headquarters. Your comments will receive careful consider-
ation at a meeting of the responsible technical committee, which you may attend. is held in a shallow dish and placed in a cage that provides at
3 3
Thesolesourceofsupplyofthemedium(CSMAStandardFlyMe
...