EN ISO 734-1:2000

Oilseed residues - Determination of oil content - Part 1: Extraction method with hexane (or light petroleum) (ISO 734-1:1998)

Ölsamenrückstände - Bestimmung des Ölgehaltes - Teil 1: Extraktionsverfahren mit Hexan (oder Petrolether) (ISO 734-1:1998)

Dieser Teil von ISO 734 legt ein Verfahren zur Bestimmung des Hexanextrakts (oder des Petroletherextrakts), genannt "Ölgehalt", von Extraktionsschroten (ausgeschlosen sind Mischprodukte) fest, die durch Extraktion von Öl aus Ölsamen durch Pressen oder Lösungsmittel erhalten wurden.

Tourteaux de graines oléagineuses - Détermination de la teneur en huile - Partie 1: Méthode par extraction à l'hexane (ou à l'éther de pétrole) (ISO 734-1:1998)

Ostanki tropin - Določevanje olja - 1. del: Metoda ekstrakcije s heksanom (ali petroletrom) (ISO 734-1:1998)

General Information

• Status: Withdrawn
• Publication Date: 22-Aug-2000
• Withdrawal Date: 31-Oct-2006
• ICS: 67.200.20 - Oilseeds
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Start Date: 01-Nov-2006
• Completion Date: 01-Nov-2006
• Ref Project: SIST EN ISO 734-1:2001 - Oilseed residues - Determination of oil content - Part 1: Extraction method with hexane (or light petroleum) (ISO 734-1:1998)

Relations

• Replaced By: EN ISO 734-1:2006 - Oilseed meals - Determination of oil content - Part 1: Extraction method with hexane (or light petroleum) (ISO 734-1:2006)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 734-1:2001
01-februar-2001
2VWDQNLWURSLQ'RORþHYDQMHROMDGHO0HWRGDHNVWUDNFLMHVKHNVDQRPDOL
SHWUROHWURP,62
Oilseed residues - Determination of oil content - Part 1: Extraction method with hexane
(or light petroleum) (ISO 734-1:1998)
Ölsamenrückstände - Bestimmung des Ölgehaltes - Teil 1: Extraktionsverfahren mit
Hexan (oder Petrolether) (ISO 734-1:1998)
Tourteaux de graines oléagineuses - Détermination de la teneur en huile - Partie 1:
Méthode par extraction a l'hexane (ou a l'éther de pétrole) (ISO 734-1:1998)
Ta slovenski standard je istoveten z: EN ISO 734-1:2000
ICS:
67.200.20 Oljnice Oilseeds
SIST EN ISO 734-1:2001 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 734-1:2001

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SIST EN ISO 734-1:2001

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SIST EN ISO 734-1:2001

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SIST EN ISO 734-1:2001
INTERNATIONAL ISO
STANDARD 734-1
First edition
1998-08-15
Oilseed residues — Determination of oil
content
Part 1:
Extraction method with hexane (or light
petroleum)
Tourteaux de graines oléagineuses — Détermination de la teneur en
huile —
Partie 1: Méthode par extraction à l'hexane (ou à l'éther de pétrole)
A
Reference number
ISO 734-1:1998(E)

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SIST EN ISO 734-1:2001
ISO 734-1:1998(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation of national standards bodies (ISO member bodies). The work of
preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which
a technical committee has been established has the right to be represented
on that committee. International organizations, governmental and non-
governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 734-1 was prepared by Technical Committee
ISO/TC 34, Agricultural food products, Subcommittee SC 2, Oleaginous
seeds and fruits.
This first edition of ISO 734-1, together with ISO 734-2, cancels and
replaces ISO 734:1979, which has been technically revised.
ISO 734 consists of the following parts, under the general title Oilseed
residues — Determination of oil content:
 Part 1: Extraction method with hexane (or light petroleum)
 Part 2: Rapid extraction method
Annexes A and B of this part of ISO 734 are for information only.
©  ISO 1998
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 • CH-1211 Genève 20 • Switzerland
Internet [e-mail]
Printed in Switzerland
ii

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SIST EN ISO 734-1:2001
©
ISO ISO 734-1:1998(E)
Introduction
As the determination of the "oil content" of oilseeds is carried out according
to ISO 659 [1], it has been considered necessary to provide for the control
of oil production by establishing a reference method for the determination
of "oil content" of oilseed residues in the same way.
However, the principal users of oilseed residues, namely the
manufacturers of animal feeding stuffs, carry out the determination by
extraction with diethyl ether in some countries, but with n-hexane or light
petroleum in others. The two methods do not always give the same results,
and large amounts of data relating to the formulation of animal feeding
stuffs are based on each of the two methods. It was therefore considered
necessary to establish a separate International Standard dealing with the
method using diethyl ether, and that is the subject of ISO 736 [2].
iii

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SIST EN ISO 734-1:2001

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SIST EN ISO 734-1:2001
©
INTERNATIONAL STANDARD  ISO ISO 734-1:1998(E)
Oilseed residues — Determination of oil content —
Part 1:
Extraction method with hexane (or light petroleum)
1  Scope
This part of ISO 734 specifies a method for the determination of the hexane extract (or light-petroleum extract),
called "oil content", of residues (excluding compounded products) obtained by the extraction of oil from oilseeds by
pressure or solvent.
2  Normative references
The following standards contain provisions which, through reference in this text, constitute provisions of this part of
ISO 734. At the time of the publication, the editions indicated were valid. All standards are subject to revision, and
parties to agreements based on this part of ISO 734 are encouraged to investigate the possibility of applying the
most recent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid
International Standards.
ISO 771:1977, Oilseed residues — Determination of moisture and volatile matter content.
ISO 5502:1992, Oilseed residues — Preparation of test samples.
3  Definition
For the purposes of this part of ISO 734, the following definition applies.
3.1
oil content
whole of the substances extracted under the operating conditions specified in this part of ISO 734, and expressed
as a percentage by mass of the product as received
NOTE  On request, the oil content may be expressed relative to dry matter.
4  Principle
Extraction of a test portion of the product in a suitable apparatus, with technical hexane or, failing this, light
petroleum. Elimination of the solvent and weighing of the extract obtained.
5  Reagents
Use only reagents of recognized analytical grade, unless otherwise specified.
1

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SIST EN ISO 734-1:2001
©
ISO 734-1:1998(E) ISO
5.1  Technical hexane, n-hexane or light petroleum, essentially composed of hydrocarbons with 6 carbon
atoms, of which less than 5 % distils below 50 °C and more than 95 % distils between 50 °C and 70 °C.
For either solvent, the residue on complete evaporation shall not exceed 2 mg per 100 ml.
6  Apparatus
Usual laboratory apparatus and, in particular, the following.
6.1  Mechanical grinder, easy to clean and allowing the residues to be ground, without heating and without
appreciable change in moisture, volatile matter and oil content, to obtain particles passing completely through a
sieve of aperture size 1 mm.
1)
6.2  Mechanical microgrinder, of the Dangoumau type capable of producing a fineness of grinding of oilseeds
residues of less than 160 μm, with the exception of the "shell", particles of which may reach 400 μm.
NOTE  In laboratories where a microgrinder is not available, microgrinding of the ground sample (see 9.3.3) may be replaced
by trituration with a pestle and mortar, in the presence of about 10 g of sand which has been washed with hydrochloric acid and
then calcined. However, grinding in a mortar cannot be applied in the case of multiple analyses because operator fatigue
prevents sufficiently efficient grinding of numerous samples, and the extraction of oil from a coarsely ground sample can never
be complete.
6.3  Extraction thimble and cotton wool, or filter paper, free from matter soluble in hexane or light petroleum.
6.4  Suitable extraction apparatus, fitted with a flask of capacity 200 ml to 250 ml.
2)
NOTE Straight-through extractors, for example the Butt, Smalley, Twisselmann and Bolton-Williams , are suitable.
The use of other extractors is conditional upon the results of a test on a standard material of known oil content to confirm the
suitability of the apparatus.
6.5  Electric heating bath (sand bath, water bath, etc.) or hot-plate.
6.6  Electrically heated oven, with thermostatic control, permitting ventilation or obtaining reduced pressure,
capable of being maintained at 103 °C 6 2 °C.
6.7  Desiccator, containing an efficient desiccant.
6.8  Pumice stone, in small particles, previously dried in an oven at 103 °C 6 2 °C then cooled in a desiccator.
6.9  Analytical balance, capable of weighing to an accuracy of 6 0,001 g.

1)
The Dangoumau mechanical microgrinder is example of suitable product available commercially. This information is given

for the convenience or users of this part of ISO 734 and does not constitute an endorsement by ISO of this product.
2)
 The Butt, Smalley, Twisselmann or Bolton-Willi
...