This document specifies a method for the determination of the moisture and volatile matter content of oilseed meals obtained by the extraction of oil from oilseeds by pressure and/or solvent.

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EN-ISO 665 specifies a method for the determination of the moisture and volatile matter content of oilseeds.

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This document specifies a method for the determination of the moisture and volatile matter content of oilseeds.

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This document specifies a method for the determination of the moisture and volatile matter content of oilseeds.

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This document specifies certain characteristics of the essential oil of aniseed (Pimpinella anisum L.), with a view to facilitating the assessment of its quality.

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This document specifies a rapid method for extraction of oil and for preparation of the methyl esters of fatty acids. The methyl esters thus obtained can be used for gas chromatography. This International Standard is applicable to the following oilseeds: rape, sunflower, soya beans, mustard, linseed. NOTE Applying this rapid method to high erucic acid content rapeseed leads to an overestimation of erucic acid.

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This document specifies a rapid method for extraction of oil and for preparation of the methyl esters of fatty acids. The methyl esters thus obtained can be used for gas chromatography.
This document is applicable to the following oilseeds: rape and mustard with low erucic acid content (< 2 %), sunflower, soya beans, linseed.
NOTE Applying this rapid method to high erucic acid content rapeseed leads to an overestimation of erucic acid content by approximately a mass fraction of 1 %. This difference was observed in Reference [6] and could be due to the partial extraction of the oil from the sample (yield around 70 %). High content of erucic acid in triglycerides could increase their solubility in hexane because of the lipophilic effect of the carbon long-chain (C22). However, as this effect was not checked on a large set of high erucic rapeseed samples, it is not appropriate to apply a correction factor to the erucic acid content when analysing high erucic acid rapeseed.

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This document specifies a rapid method for extraction of oil and for preparation of the methyl esters of fatty acids. The methyl esters thus obtained can be used for gas chromatography. This document is applicable to the following oilseeds: rape and mustard with low erucic acid content ( NOTE Applying this rapid method to high erucic acid content rapeseed leads to an overestimation of erucic acid content by approximately a mass fraction of 1 %. This difference was observed in Reference [6] and could be due to the partial extraction of the oil from the sample (yield around 70 %). High content of erucic acid in triglycerides could increase their solubility in hexane because of the lipophilic effect of the carbon long-chain (C22). However, as this effect was not checked on a large set of high erucic rapeseed samples, it is not appropriate to apply a correction factor to the erucic acid content when analysing high erucic acid rapeseed.

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This document specifies a method for the determination of the individual glucosinolates content in
rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.
This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica
juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates
previously identified are described in this document. On the contrary, the quantitative analysis of the
concerned glucosinolate(s) is not carried out.
NOTE This method does not determine glucosinolates that are substituted on the glucose molecule, but
these compounds are of little importance in commercial rapeseed and rapeseed meal.
Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method.
Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents
how to prepare and test the purified sulfatase solution and how to check the desulphation step on the
ion exchange column. Annex D presents the HPLC and column performance criteria qualification.
The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode.
This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers),
as described in Annex E.

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This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.
This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out.
NOTE This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.
Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification.
The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.

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This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution. This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out. NOTE This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal. Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification. The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.

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This document specifies a method of determining the urease activity of products derived from soya beans. The method allows inadequate cooking of these products to be detected. It is applicable to products having a urease activity of less than 1 mg of nitrogen per gram of product as received, under the conditions specified. For more active products, the method is applicable provided that the mass of the test portion is reduced (see 9.1).

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This document specifies the requirements for discontinuous sampling of oilseeds, using the manual or
automatic method, for the purpose of assessing their quality and condition.
NOTE An example of “condition” is an odour due to a treatment product.

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ISO 21294:2017 specifies the requirements for discontinuous sampling of oilseeds, using the manual or automatic method, for the purpose of assessing their quality and condition.
NOTE An example of "condition" is an odour due to a treatment product.

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ISO 21294:2017 specifies the requirements for discontinuous sampling of oilseeds, using the manual or automatic method, for the purpose of assessing their quality and condition. NOTE An example of "condition" is an odour due to a treatment product.

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This International Standard specifies a method for the determination of soluble proteins in potassium hydroxide solution in soya meals, rapeseed meals and sunflower pellets, which are then assayed using the Kjeldahl method as specified in ISO 5983-1 and ISO 5983-2.

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ISO 14244:2014 specifies a method for the determination of soluble proteins in potassium hydroxide solution in soya meals, rapeseed meals and sunflower pellets, which are then assayed using the Kjeldahl method as specified in ISO 5983-1 and ISO 5983-2.

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This International Standard specifies a method for the determination of the hexane extract (or lightpetroleum extract), called “oil content”, of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvents.

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This International Standard specifies an extraction method which may be used to assess the efficiency
of a de-oiling process by comparing the oil content of the oilseed with the residual oil content of the
corresponding extraction meals, pellets and expeller cakes.
It is not applicable to disputed cases, for which ISO 734 is applicable.
It is applicable to oilseed meals obtained from oilseeds by expelling or by extraction with a solvent, as
well as to the pellets made from the residues.

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ISO 734:2015 specifies a method for the determination of the hexane extract (or light-petroleum extract), called "oil content", of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvents.

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ISO 22630:2015 specifies an extraction method which may be used to assess the efficiency of a de-oiling process by comparing the oil content of the oilseed with the residual oil content of the corresponding extraction meals, pellets and expeller cakes.
It is not applicable to disputed cases, for which ISO 734 is applicable.
It is applicable to oilseed meals obtained from oilseeds by expelling or by extraction with a solvent, as well as to the pellets made from the residues.

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ISO 734:2015 specifies a method for the determination of the hexane extract (or light-petroleum extract), called "oil content", of meals (excluding compounded products) obtained by the extraction of oil from oilseeds by pressure or solvents.

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ISO 22630:2015 specifies an extraction method which may be used to assess the efficiency of a de-oiling process by comparing the oil content of the oilseed with the residual oil content of the corresponding extraction meals, pellets and expeller cakes. It is not applicable to disputed cases, for which ISO 734 is applicable. It is applicable to oilseed meals obtained from oilseeds by expelling or by extraction with a solvent, as well as to the pellets made from the residues.

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This International Standard specifies a spectrometric method for the determination of the chlorophyll
content of rapeseed. It is not applicable to the determination of chlorophyll in oils.

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ISO 10519:2015 specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.

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ISO 10519:2015 specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.

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ISO 14244:2014 specifies a method for the determination of soluble proteins in potassium hydroxide solution in soya meals, rapeseed meals and sunflower pellets, which are then assayed using the Kjeldahl method as specified in ISO 5983-1 and ISO 5983-2.

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This International Standard specifies a reference method for the determination of the hexane extract (or light petroleum extract), called "oil content", of oilseeds used as industrial raw materials. The procedure for sunflower seeds is different from the other seeds as it includes a second moisture content determination after the first mechanical grind. The method has been tested on rapeseed, soya beans and sunflower seeds. This does not, however, preclude its applicability to other commercial seeds. If required, the pure seeds and the impurities (see 9.4) may be analysed separately. In the case of groundnuts, the pure seeds, the total fines, the non-oleaginous impurities and the oleaginous impurities (see 10.1.6) may be analysed separately.

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ISO 659:2009 specifies a reference method for the determination of the hexane extract (or light petroleum extract), called the “oil content”, of oilseeds used as industrial raw materials. The procedure for sunflower seed is different from those for other seeds as it includes an additional moisture content determination after the seed has been ground to prepare the test sample.
The method has been tested on rapeseed, soya beans and sunflower seed. This does not, however, preclude its applicability to other commercial seeds.
If required, the pure seeds and the impurities can be analysed separately. In the case of groundnuts, the pure seeds, the total fines, the non-oleaginous impurities and the oleaginous impurities can be analysed separately.

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ISO 659:2009 specifies a reference method for the determination of the hexane extract (or light petroleum extract), called the “oil content”, of oilseeds used as industrial raw materials. The procedure for sunflower seed is different from those for other seeds as it includes an additional moisture content determination after the seed has been ground to prepare the test sample. The method has been tested on rapeseed, soya beans and sunflower seed. This does not, however, preclude its applicability to other commercial seeds. If required, the pure seeds and the impurities can be analysed separately. In the case of groundnuts, the pure seeds, the total fines, the non-oleaginous impurities and the oleaginous impurities can be analysed separately.

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This part of ISO 16634 specifies a method for the determinatio n of the total nitrogen content and the calculation of crude p rotein content of oilseeds and animal feeding stuffs. This met hod, like the Kjeldahl method, does not distinguish between pr otein nitrogen and non-protein nitrogen. For the calculation o f protein content, various conversion factors are used (see An nex D).

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ISO 16634-1:2008 specifies a method for the determination of the total nitrogen content and the calculation of crude protein content of oilseeds and animal feeding stuffs. This method, like the Kjeldahl method, does not distinguish between protein nitrogen and non-protein nitrogen. For the calculation of protein content, various conversion factors are used. This method is not applicable to milk and milk products.

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This European Standard specifies the procedure for obtaining a test sample from a laboratory sample of oilseeds. NOTE Some contracts for the trading of oilseeds call for analyses of the sample as drawn, i.e. including any impurities that may be present. However, some contracts call for the preliminary quantitative separation of impurities and analysis of the pure seed separated. Analysis of the impurities may also be required.

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ISO 664:2008 specifies the procedure for obtaining a test sample from a laboratory sample of oilseeds.

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ISO 664:2008 specifies the procedure for obtaining a test sample from a laboratory sample of oilseeds.

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Migrated from Progress Sheet (TC Comment) (2000-07-10): BP : Awaiting for precision data ( a ring test is in progress) (NT/1998-09-17) - STDDEL Note: Use Bitmap Settings

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This International Standard specifies a method for the determination of the impurities content in oilseeds used as primary industrial materials. It also defines the various categories of what are usually understood to be impurities.

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This International Standard specifies a method for the determination of the impurities content in oilseeds used as primary industrial materials. It also defines the various categories of what are usually understood to be impurities.

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This International Standard specifies a rapid method using nuclear magnetic resonance (NMR) for the determination of the oil and water contents of oilseed residues obtained after oil extraction by pressure or solvents (excluding mixed products). It is applicable to oilseed residues as flour, plates or agglomerates, provided that the particles are smaller than 2 mm and that the water content is not higher than the conversion threshold.

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Specifies a method for the determination of hexane content after extraction with hydrocarbon-based solvents. Describes the principle, the reagents and materials, the apparatus, the sampling, the test procedure, the expression of results, and the contents of the test report.

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This International Standard specifies a rapid method for the determination of the oil and water contents of commercial oilseeds using pulsed nuclear magnetic resonance (NMR). It is applicable to rapeseeds, soya beans, linseeds and sunflower seeds with a water content less than 10 %. For seeds with higher water contents, drying is necessary before the oil content can be determined by pulsed NMR. NOTE 1 This method has been tested with rapeseeds, soya beans, linseeds and sunflower seeds. This does not, however, preclude its applicability to other commercial seeds whose oil is liquid at the temperature of measurement. NOTE 2 The reproducibility values are generally higher than those obtained by the drying method (ISO 665)

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Specifies a method for the determination of the content of the different glucosinolates in crucifer oilseeds. Does not allow to determine glucosinolates which are substituted on the glucose molekule.

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Specifies a method for the determination of hexane content after extraction with hydrocarbon-based solvents. Describes the principle, the reagents and materials, the apparatus, the sampling, the test procedure, the expression of results, and the contents of the test report.

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The method consists in dissolving in a mixture of diethyl ether and ethanol of the oil extracted for the determination of the "oil contend" of the seeds (see ISO 659), and then titrating of the free fatty acids present using an ethanolic potassium hydroxide solution. It is not applicable to cotton seeds with adherent cotton linters, or to palm or olive fruits.

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The principle of the method is grinding of the laboratory sample, with or without preliminary breaking, crushing, grinding or drying, division of the sample by suitable means, taking care that the test sample thus obtained, from which the test portion(s) will be taken, truly represents the totality of the laboratory sample.

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Specifies a method for the determination of hexane content after extraction with hydrocarbon-based solvents. Describes the principle, the reagents and materials, the apparatus, the sampling, the test procedure, the expression of results, and the contents of the test report.

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Specifies general conditions relating to the sampling for the assessment of quality of oilseeds purchased as industrial raw materials. Nine figures show exemples of suitable apparatus. There is specified the limitationof the size of lot, methods of taking samples, packaging and labelling of samples, the dispatch of samples and the sampling report.

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