EN ISO 10519:2015

Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2015)

ISO 10519:2015 specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.

Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren (ISO 10519:2015)

Graines de colza - Détermination de la teneur en chlorophylle - Méthode spectrométrique (ISO 10519:2015)

ISO 10519:2015 spécifie une méthode de spectrométrie permettant de déterminer la teneur en chlorophylle des graines de colza. Elle n'est pas applicable à la détermination de la chlorophylle dans les huiles.

Seme oljne repice - Določevanje klorofila - Spektrometrijska metoda (ISO 10519:2015)

Ta mednarodni standard določa spektometrijsko metodo za določevanje klorofila v semenu oljne repice. Ne uporablja se za določevanje klorofila v oljih.

General Information

• Status: Published
• Publication Date: 29-Sep-2015
• Withdrawal Date: 30-Mar-2016
• ICS: 67.200.20 - Oilseeds
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 30-Sep-2015
• Completion Date: 30-Sep-2015
• Ref Project: SIST EN ISO 10519:2015 - Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2015)

Relations

• Replaces: EN ISO 10519:2000 - Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:1997)
• Effective Date: 07-Oct-2015
• Amended By: EN ISO 10519:2015/A1:2025 - Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor (ISO 10519:2015/Amd 1:2025)
• Effective Date: 21-Jun-2023

Overview

EN ISO 10519:2015 - Rapeseed: Determination of chlorophyll content (spectrometric method) is a CEN-adopted ISO standard that specifies a laboratory spectrometric procedure to measure chlorophyll in whole rapeseed. Published in 2015, this third edition replaces earlier versions and is intended for routine quality control and analytical work on oilseeds. The method is not applicable to determining chlorophyll in oils.

Key topics and requirements

• Scope: Spectrometric determination of chlorophyll in rapeseed (not for oils).
• Principle: Extraction of chlorophyll from a weighed test portion into a specified solvent, followed by spectrophotometric measurement and baseline correction.
• Sample and test portion: Typical test portion is 2 g of ground rapeseed prepared from a representative laboratory sample (see ISO 542 and ISO 664 for sampling and sample reduction).
• Extraction: Add 30 mL of the extraction solvent to the test vessel; extract by shaking with steel balls (1 h manually or ~20 min in a commercial ball mill). Samples must have moisture <10 % to avoid phase separation.
• Extraction solvent: A mixture of anhydrous ethanol and anhydrous ison‑heptane or petroleum hydrocarbons (see the standard for exact composition and solvent specifications).
• Spectrometric measurement: Measure absorbances at 665 nm, 705 nm and 625 nm (spectral bandwidth ≈ 2 nm). Apply baseline correction: Acorr = A665 − (A705 + A625)/2.
• Calculation: Chlorophyll content (mg/kg) is calculated from Acorr, with constant k = 13, taking into account cell path length, solvent volume and test portion mass. Dry‑basis reporting requires moisture correction per ISO 665.
• Apparatus: Analytical balance (0.001 g), grinder/microgrinder or ball mill, spectrometer (600–700 nm), optical cells (≥1 cm path), pipettes, filter paper and culture tubes.
• Precision: Repeatability limit ≈ 10 % (relative) and reproducibility limit ≈ 20 % under the conditions of the interlaboratory study reported in the standard.

Applications and users

• Who uses it: Analytical laboratories, oilseed processors, seed quality control teams, plant breeders and research institutions working on rapeseed quality.
• Practical uses: Routine quality checks on rapeseed lots, monitoring chlorophyll levels that can affect oil quality and processing, supporting compliance with commercial specifications, and research on seed maturation or harvest and storage effects.

Related standards

• ISO 542 - Sampling of oilseeds
• ISO 664 - Reduction of laboratory sample to test sample
• ISO 665 - Determination of moisture and volatile matter content
• ISO 5725 (precision methodology referenced for interlaboratory evaluation)

Keywords: EN ISO 10519:2015, rapeseed chlorophyll determination, spectrometric method, chlorophyll content, oilseeds analysis, rapeseed quality control.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren (ISO 10519:2015)Graines de colza - Détermination de la teneur en chlorophylle - Méthode spectrométrique (ISO 10519:2015)Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2015)67.200.20OljniceOilseedsICS:Ta slovenski standard je istoveten z:EN ISO 10519:2015SIST EN ISO 10519:2015en01-december-2015SIST EN ISO 10519:2015SLOVENSKI
STANDARDSIST EN ISO 10519:20011DGRPHãþD

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EN ISO 10519
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s r w s {ã t r r rEnglish Version
Rapeseed æ Determination of chlorophyll content æ Graines de colza æ Détermination de la teneur en
Rapssamen æ Bestimmung des Chlorophyllgehaltes æ This European Standard was approved by CEN on
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egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey andUnited Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
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t r s w CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN ISO
s r w s {ã t r s w ESIST EN ISO 10519:2015

ISO 10519:2015(E) ii © ISO 2015 – All rights reservedCOPYRIGHT PROTECTED DOCUMENT©
ISO 2015, Published in SwitzerlandAll rights reservedä Unless otherwise speci Ðiedá no part of this publication may be reproduced or utilized otherwise in any form or by any meansá electronic or mechanicalá including photocopyingá or posting on the internet or an intranetá without prior written permissionä Permission can be requested from either ISO at the address below or ISOïs member body in the country of the requester.ISO copyright of ÐiceCh. de Blandonnet 8 • CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. [phone]Fax [phone]copyright 7isoäorgwww.iso.orgSIST EN ISO 10519:2015

ISO 10519:2015(E) Foreword .iv1 Scope .12 Normative references .1uTermsanddeÐinitions .14 Principle .15 Reagent .15.1 Extraction solvent .16 Apparatus .17 Sampling .28 Preparation of test sample .29 Procedure.39.1 Test portions .39.2 Extraction .39.3 Determination .310 Expression of results .311 Precision .4 s sä s Repeatability .4 s sä t Reproducibility .412 Test report .4Annex A (informative) Results of interlaboratory test .5Bibliography .6© ISO 2015 – All rights reserved iiiContents PageSIST EN ISO 10519:2015

ISO 10519:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards through ISO technical committeesä Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in
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